Distribution of hepatitis B virus genotypes: Phylogenetic analysis and virological characteristics of Genotype C circulating among HBV carriers in Kolkata, Eastern India

Banerjee, Arup; Datta, Sibnarayan; Chandra, Partha K.; Roychowdhury, Susanta; Panda, Chinmoy Kumar; Chakravarty, Runu

doi:10.3748/wjg.v12.i37.5964

Cited by 44 publications

(63 citation statements)

References 39 publications

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“…A study conducted by Kumar A et al found that the genotype D was dominant over genotype A and found genotype C in one patient; this genotype has not previously been reported from India [13]. The other studies found genotype D to be the predominant genotype on genotype A [3,10]. Our results are in accordance with the latter studies which showed the dominancy of genotype D on A. HBV genotypes have been shown to have a distinct geographic distribution.…”

Section: Discussionsupporting

confidence: 91%

“…In our study it was observed that in Uttar Pradesh, North India the most prevalent genotype among patients with HBV related chronic liver disease was genotype D (78%) the remaining 22% had genotype A. HBV genotypes A and D have been well documented from different parts of mainland India [3,10,13,27]. There is not much information regarding the prevalence of HBV genotypes from North India.…”

Section: Discussionmentioning

confidence: 68%

“…In India, prevalence of HBV in chronic hepatitis is very high [8]. The HBV genotypes A and D have been reported from different parts of India [10,13,28], while C in addition to A and D has been reported and characterized recently from an eastern Indian population only [3]. Genotypes and serotypes are useful tools for understanding the epidemiology of HBV infection.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of Hepatitis B Virus Genotypes and Its Association with Severity of Liver Disease in Patients with Chronic Hepatitis B in Uttar Pradesh, India

et al. 2011

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The present study was designed to investigate the distribution of genotypes and its association with severity of liver disease in patients with Chronic Hepatitis B (CHB) in Uttar Pradesh, India. One hundred five HBsAg positive patients were selected for the study. The DNA was extracted by using pure viral DNA extraction kit. The genotype of Hepatitis B virus (HBV) was identified by using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method, by using AvaII and DpnII restriction enzyme to see the different patterns of cleavage that would occur at this specific site. Among 105 HBsAg positive chronic liver disease patients 58 patients were positive for HBeAg and 47 samples were HBeAg negative. Genotyping was done successfully in 91 samples. Genotype A was identified in 22% and genotype D in 78% CHB patients. Genotype A showed elevated liver enzymes much more than genotype D. The Child Pugh classification of 20 patients with genotype A was class A (n = 2), B (n = 9), C (n = 9) and of genotype D was class A (n = 13), B (n = 50) and C (n = 8). In conclusion our results showed that Genotype D was the commonest genotype in Uttar Pradesh, whereas genotype A had significantly more elevated ALT/AST levels than genotype D. (P \ 0.05). Child Pugh Grade B was significantly more in patients with genotype D, whereas Child Pugh Grade C was more in genotype A.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

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Distribution of Hepatitis B Virus Genotypes and Its Association with Severity of Liver Disease in Patients with Chronic Hepatitis B in Uttar Pradesh, India

et al. 2011

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“…4 This population formed clusters distinctly from the North-East population groups (ethnic affiliation to Mongoloid), who are possibly descendents of ancestral population of China. 5 Thus, the history of Bangladesh combines Indo-Aryan, Austro-Asiatic, Dravidian, Mughal, Arab, Persian, Turkic and British influences. 6 There are several ethnic groups in Bangladesh, where 98% are Bengali and the remaining 2% belong to the Chakma, Bawm, Garo, Kiri, Marma, Tipuri and other ethnic minorities.…”

Section: Introductionmentioning

confidence: 99%

Human Leukocyte Antigen: Class I Allele Frequencies and Haplotype Distributionin a Tertiary Care Hospital in Bangladesh

Sultana

Tabassum

Nessa

2018

Bangladesh Med Res Counc Bull

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Human leukocyte antigen (HLA) are cell surface glycoproteins encoded by Major Histocompatibility Complex (MHC) gene of human genome. HLA antigen frequency and haplotype distribution are useful for determining disease association, origin, migration and genetic relationship between populations and predicting the outcome of transplantation. Thus, the present study was carried out to identify HLA class I (HLA-A and HLA-B) antigen and haplotype distribution among a selected Bangladeshi population. This retrospective study was conducted among 1070 individuals, who were referred by clinicians for HLA typing at the Tissue Typing Laboratory of the Department of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU) during the period 2009 to 2011. For HLA typing, blood was collected in heparin containing tube and the laboratory tests were performed by the microlymphocytotoxicity technique according to manufacturer's instructions. Out of 19 HLA-A and 37 HLA-B antigens tested, a total of 19/19 and 36/37 antigens were detected respectively in this study. The most frequent antigens of HLA-A and HLA-B detected were A11 (25.4%), A24 (16.6%), B75 (18.1%) and B35 (11.3%). The least antigen frequency detected for HLA-A locus were A69 (0.09%), A26 (0.28%), A34 (0.28%), while for HLA-B locus were B81 (0.09%) and B56 (0.09%). Among the HLA-A and HLA-B antigens, some alleles were found to be homozygote such as A11 (4.0%), A2 (2.7%), A24 (2.1%) and B75 (2.4%), B35 (1.8%), and B44 (1.4%) respectively. The most frequent haplotype in the study population were A11: B75 (4.9%). The most frequent antigens of HLA-A and HLA-B detected were A11 (25.4%), B75 (18.1%) respectively. The distribution of HLA haplotypes among the study population indicates that it has the influence of Oriental and Asian populations. Thus, this study will be helpful to provide valuable information for population genetics and HLA disease association analysis.

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“…Some reports have found that C-terminal truncated S proteins exist in patients with chronic hepatitis B infection and hepatocellular carcinoma [34] . The shorted S proteins can stimulate gene expression from the endogenous HBV enhancer Ⅰ and can also deregulate the expression of oncogenes such as c-myc [35] , which may help clarify the tumor development mechanism of HBV. Whether the truncated S proteins www.wjgnet.com…”

Section: Cacatttcctgmentioning

confidence: 99%

Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

Shen¹,

Yan²,

Zou³

et al. 2008

WJG

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AIM:To determine whether HBV with the same characteristics causes dissimilar mutations in different hosts. METHODS: Full-length HBV genome was amplified and linked with pMD T18 vector. Positive clones were selected by double-restriction endonuclease digestion (Eco RⅠ and Hin dⅢ) and PCR. Twenty seven clones were randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and 6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis was performed using BioEditor, Clustal X and MEGA software. Potential immune epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method and Emini Surface Accessibility Prediction. RESULTS: All of the 27 sequences were genotype C, the divergence between the mother and son was 0%-0.8%. Compared with another 50 complete sequences of genotype C, the mother and her son each had 13 specific nucleotides that differed from the other genotype C isolates. AA 1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The 1762T/1764A double mutation existed in all clones of the mother, 3 of them were also coupled with G1896A mutation, but none were found in the son. 17 bp deletion starting at nucleotide 2330 was the major mutation (5/9) in the son, which caused seven potential HLA class Ⅰ epitopes and one B cell epitope deletion, and produced a presumptive new start codon, downstream from the original one of the P gene. CONCLUSION: The HBV strain in the son came from his mother, and discrepant mutation occurred in the mother and her son during infection.

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Distribution of hepatitis B virus genotypes: Phylogenetic analysis and virological characteristics of Genotype C circulating among HBV carriers in Kolkata, Eastern India

Cited by 44 publications

References 39 publications

Distribution of Hepatitis B Virus Genotypes and Its Association with Severity of Liver Disease in Patients with Chronic Hepatitis B in Uttar Pradesh, India

Distribution of Hepatitis B Virus Genotypes and Its Association with Severity of Liver Disease in Patients with Chronic Hepatitis B in Uttar Pradesh, India

Human Leukocyte Antigen: Class I Allele Frequencies and Haplotype Distributionin a Tertiary Care Hospital in Bangladesh

Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

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