Distribution of heterocyst glycolipids in cyanobacteria

Bauersachs, Thorsten; Compaoré, Justine; Hopmans, Ellen C.; Stal, Lucas J.; Schouten, Stefan; Damsté, Jaap S. Sinninghe

doi:10.1016/j.phytochem.2009.08.014

Cited by 69 publications

(106 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, in view of the inferred marine setting of the site during the Eocene, atmospheric nitrogen fixation by these N 2 -fixing heterocystous symbiotic cyanobacteria probably played an important role in supplying fixed nitrogen and sustaining Azolla growth in the strongly stratified Eocene Arctic Ocean. However, negative 15 N-values are also observed during times of low Azolla counts and HG concentrations, as observed previously (29), suggesting that N 2 -fixation was a persistent feature at these times (29, 31). …”

Section: Discussionsupporting

confidence: 79%

“…and Nodularia spp. (15) were detected in microbial mats from the North Sea barrier island Schiermonnikoog (Table S1). We then determined the fate of HGs by screening sediments from a number of modern environments, known to host heterocystous cyanobacteria, for the presence of HGs (see SI Text).…”

Section: Resultsmentioning

confidence: 99%

“…HGs were also identified within the boundaries of the S1 sapropel, albeit in lower abundances, in agreement with less depleted bulk δ 15 N values (þ0.4‰). Some differences in the distribution of the individual HGs in the S1 and S5 sapropels are noted (Table S2), which may either reflect adaptations to different environmental conditions, such as temperature and oxygen concentration (15), or contributions of different heterocystous cyanobacteria (15) at times of sapropel formation.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Fossilized glycolipids reveal past oceanic N ₂ fixation by heterocystous cyanobacteria

Bauersachs

Speelman

Hopmans

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

N 2 -fixing cyanobacteria play an essential role in sustaining primary productivity in contemporary oceans and freshwater systems. However, the significance of N 2 -fixing cyanobacteria in past nitrogen cycling is difficult to establish as their preservation potential is relatively poor and specific biological markers are presently lacking. Heterocystous N 2 -fixing cyanobacteria synthesize unique long-chain glycolipids in the cell envelope covering the heterocyst cell to protect the oxygen-sensitive nitrogenase enzyme. We found that these heterocyst glycolipids are remarkably well preserved in (ancient) lacustrine and marine sediments, unambiguously indicating the (past) presence of N 2 -fixing heterocystous cyanobacteria. Analysis of Pleistocene sediments of the eastern Mediterranean Sea showed that heterocystous cyanobacteria, likely as epiphytes in symbiosis with planktonic diatoms, were particularly abundant during deposition of sapropels. Eocene Arctic Ocean sediments deposited at a time of large Azolla blooms contained glycolipids typical for heterocystous cyanobacteria presently living in symbiosis with the freshwater fern Azolla, indicating that this symbiosis already existed in that time. Our study thus suggests that heterocystous cyanobacteria played a major role in adding "new" fixed nitrogen to surface waters in past stratified oceans.nitrogen fixation | intact polar lipids | cyanobacterial biomarkers | symbiosis | saproprel

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fossilized glycolipids reveal past oceanic N ₂ fixation by heterocystous cyanobacteria

Bauersachs

Speelman

Hopmans

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although not produced by all cyanobacteria species (Gugger et al 2002), the more specific PLFA C18:3x6, which has previously been used as a marker for cyanobacteria, was either not present or detected in very low abundances in our samples. Heterocyst glycolipids (HGs) have also been studied as cyanobacterial markers (Bauersachs et al 2009), but due to the work-up process prior to PLFA analyses it was unfortunately no longer possible to analyse HGs on our samples. Also, we were unable to trace methanotrophic bacteria using PLFAs.…”

Section: Discussionmentioning

confidence: 99%

Carbon flows in eutrophic Lake Rotsee: a 13C-labelling experiment

et al. 2016

View full text Add to dashboard Cite

The microbial segment of food webs plays a crucial role in lacustrine food-web functioning and carbon transfer, thereby influencing carbon storage and CO 2 emission and uptake in freshwater environments. Variability in microbial carbon processing (autotrophic and heterotrophic production and respiration based on glucose) with depth was investigated in eutrophic, methane-rich Lake Rotsee, Switzerland. In June 2011, 13 C-labelling experiments were carried out at six depth intervals in the water column under ambient light as well as dark conditions to evaluate the relative importance of (chemo)autotrophic, mixotrophic and heterotrophic production. Label incorporation rates of phospholipid-derived fatty acid (PLFA) biomarkers allowed us to differentiate between microbial producers and calculate group-specific production. We conclude that at 6 m, net primary production (NPP) rates were highest, dominated by algal photoautotrophic production. At 10 m -the base of the oxycline-a distinct low-light community was able to fix inorganic carbon, while in the hypolimnion, heterotrophic production prevailed. At 2 m depth, high label incorporation into POC could only be traced to nonspecific PLFA, which prevented definite identification, but suggests cyanobacteria as dominating organisms. There was also depth zonation in extracellular carbon release and heterotrophic bacterial growth on recently fixed carbon. Large differences were observed between concentrations and label incorporation of POC and biomarkers, with large pools of inactive biomass settling in the hypolimnion, suggesting late-/post-bloom conditions. Net primary production (115 mmol C m -2 d -1 ) reached highest values in the epilimnion and was higher than glucose-based production (3.3 mmol C m -2 d -1 , highest rates in the hypolimnion) and respiration (5.9 mmol C m -2 d -1 , highest rates in the epilimnion). Hence, eutrophic Lake Rotsee was net autotrophic during our experiments, potentially storing large amounts of carbon.

show abstract

“…Two examples are the C 26 -C 32 alkyl chains, containing hydroxyl and ketone functional groups found in heterocyst glycolipids of filamentous cyanobacteria (Bauersachs et al, 2009;Campbell, Cohen, & Meeks, 1997), and the C 22 -C 26 chains of phenolic lipids of dormant cysts of the Gram-negative bacterium Azotobacter vinelandii (order Pseudomonadales) (Miyanaga, Funa, Awakawa, & Horinouchi, 2008).…”

Section: Fatty Acid Synthasementioning

confidence: 99%

Marine Microbial Secondary Metabolites

Giordano

Coppola

Russo

et al. 2015

Advances in Microbial Physiology

View full text Add to dashboard Cite

Distribution of heterocyst glycolipids in cyanobacteria

Cited by 69 publications

References 16 publications

Fossilized glycolipids reveal past oceanic N ₂ fixation by heterocystous cyanobacteria

Fossilized glycolipids reveal past oceanic N ₂ fixation by heterocystous cyanobacteria

Carbon flows in eutrophic Lake Rotsee: a 13C-labelling experiment

Marine Microbial Secondary Metabolites

Contact Info

Product

Resources

About

Distribution of heterocyst glycolipids in cyanobacteria

Cited by 69 publications

References 16 publications

Fossilized glycolipids reveal past oceanic N 2 fixation by heterocystous cyanobacteria

Fossilized glycolipids reveal past oceanic N 2 fixation by heterocystous cyanobacteria

Carbon flows in eutrophic Lake Rotsee: a 13C-labelling experiment

Marine Microbial Secondary Metabolites

Contact Info

Product

Resources

About

Fossilized glycolipids reveal past oceanic N ₂ fixation by heterocystous cyanobacteria

Fossilized glycolipids reveal past oceanic N ₂ fixation by heterocystous cyanobacteria