Distribution of human group a rotavirus VP7 and VP4 types circulating in Seoul, Korea between 1998 and 2000

Song, Mi-Ok; Kim, Ki‐Jung; Chung, Sunyang; Lim, Inseok; Kang, Shien-Young; An, Chang-Nam; Kim, Wonyong

doi:10.1002/jmv.10398

Cited by 34 publications

(25 citation statements)

References 38 publications

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“…However, a high incidence of type P [6] strains, as seen in this study, was also reported among infants with diarrhea in Nigeria, India, and Brazil (1,12,16). An incidence of type P [6] comparable to that found in this study was also reported in the study previously conducted in South Korea (15). In this study, types P [9] and P [10] were found for the first time in Korea.…”

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confidence: 90%

“…We also noticed the emergence of type G4 in the present study. Another study previously conducted in Korea showed an even higher incidence of type G4 (40.9%), although the study was performed with samples from only one city (15). The G types of minor incidence in our study included three strains of type G3 (0.9%) and one strain of type G9 but no strain of type G8, unlike the recent emergence of type G9 in several other countries (2,7,13).…”

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confidence: 65%

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Surveillance Study (2000 to 2001) of G- and P-Type Human Rotaviruses Circulating in South Korea

et al. 2004

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Human rotavirus VP4 and VP7 gene sequences were amplified by reverse transcription-PCR from 53% (322 of 607) of fecal specimens collected from children with severe diarrhea who visited hospitals in six urban areas of South Korea in 2000 and 2001. G2 was the most frequently found G type (constituted 50.6%), followed by G1 (30.1%) and G4 (13.0%). Although the P types of high incidence were P[4] (53.1%) and P[8] (21.4%), a significant incidence of P[6] (20.2%) was also noticeable. The commonest G-and P-type combination found in this study was G2P[4], rather than G1P[8], the most prevalent type known worldwide.Rotaviruses are the major etiological agent of gastroenteritis and cause vomiting, diarrhea, and fever in infants and young children worldwide (8). Rotaviruses are divided into seven groups (groups A to G) on the basis of their antigenic properties. Group A rotaviruses are further divided into the G and the P subtypes according to the antigenic property of the VP7 protein (glycoprotein) and the VP4 protein (protease-susceptible protein), respectively. To date, 10 G types have been identified in humans, but most cases of human infection are associated with four G types (types G1 to G4), of which G1 is the most prevalent worldwide (5, 10). It is well established that the G serotypes coincide with the G genotypes, while P serotypes are classified by a system different from that used to classify P genotypes. Types P[4] and P[8] are most frequently found in humans (according to convention, P genotypes are indicated with brackets). Recent studies, however, indicated the emergence of novel P types in different parts of world (3,11) Rotavirus infection is the most common cause of acute diarrhea in infants and young children in South Korea (14). To monitor the diversity of rotavirus strains circulating in the country, we carried out an analysis of 607 fecal specimens collected from infants and young children with acute diarrhea who visited 18 urban hospitals and four clinical laboratories scattered around the six provinces of the country from January 2000 to April 2001. The fecal suspension (10% in phosphatebuffered saline) was centrifuged at 10,000 ϫ g for 10 min, and the double-stranded viral RNA was extracted by treatment of the supernatant with phenol-chloroform-1% sodium dodecyl sulfate, as described previously (6). Reverse transcription (RT)-PCR was performed with the consensus primers Beg9 and End9 to amplify the VP7 gene sequence in full (1,062 bp) and primers Con2 and Con3 to amplify the VP4 gene sequence (877 bp). A second-round, multiplex PCR was performed with primers specific for G types (G1, G2, G3, G4, G8, and G9) and P types (P[4], P[6], P[8], P[9], and P[10]), as described previously (4, 6). The G and P types were determined from the migration rates of the amplicons in a 1.2% agarose gel. Among the 607 stool samples 322 (53.0%) were positive for rotavirus RNA by RT-PCR. The presence of rotaviral antigens was determined by a latex agglutination assay (Biomerieux, Marcy l'Etoile, France) and an enzyme-li...

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confidence: 90%

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confidence: 65%

Surveillance Study (2000 to 2001) of G- and P-Type Human Rotaviruses Circulating in South Korea

et al. 2004

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“…Studies in many countries indicate that serotype G1 was the most prevalent and that serotypes G2-G4, and G9 were evenly distributed (Jain et al, 2001;Hoshino et al, 2005). In Korea, G1 was the major genotype until 1997 (Kim et al, 1990;Kim, 1992Kim, , 1993, but this shifted to G4 from 1998 to 2000 (Seo and Sim, 2000;Song et al, 2003). In this study, serotype G2 (51.3%) was predominant and serotypes G1, G4 and G3 followed at 28.6%, 8.7%, and 0.9%, respectively, similar to other reports for the same period (Kim et al, 2002;So et al, 2004).…”

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confidence: 95%

Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999–2002

Moon

Green

Song

et al. 2007

Journal of Clinical Virology

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“…Until 1997, G1 was the most prevalent strain, regardless of geographic area or season [Kim et al, 1990[Kim et al, , 1999Kim, 1993;Seo and Sim, 2000]. Since 1997, the predominant G type strain shifted to G4 Song et al, 2003;Kang et al, 2005], to G2 Moon et al, 2007], and then to G9 . More recently, our data from a 2-year investigation indicated that the G1 strain has become the most common [Le et al, 2008].…”

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confidence: 97%

Genetic variation of prevalent G1P[8] human rotaviruses in South Korea

Chung

Kim

et al. 2010

Journal of Medical Virology

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The human rotavirus G1P[8] strain is one of the most common rotaviruses worldwide, including Korea. Six Korean G1P[8] human rotaviruses, isolated using cell culture techniques, were characterized on the basis of sequence differences in VP7, VP4, VP6, and NSP4 genes to elucidate the evolutionary relationships in the community. All strains had a long RNA electropherotype, supported by VP6 gene analysis, clearly associated with subgroup II specificity. The phylogenetic analysis of VP7 gene sequences showed that they all clustered into lineage I, as reported for G1 strains in Japan, China, Vietnam, and Thailand. In addition, phylogenetic analysis of the VP4 gene showed that they belong to two distinct lineages, P[8]-II and P[8]-III. With respect to the NSP4 gene, all strains belonged to genotype B. An understanding of the ecology and molecular evolution of rotaviruses circulating in the country is very important for the development of vaccines and vaccination strategies. This study provides new information concerning the genetic variability of the rotavirus strain G1P[8] occurring most commonly as a vaccine candidate.

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Distribution of human group a rotavirus VP7 and VP4 types circulating in Seoul, Korea between 1998 and 2000

Cited by 34 publications

References 38 publications

Surveillance Study (2000 to 2001) of G- and P-Type Human Rotaviruses Circulating in South Korea

Surveillance Study (2000 to 2001) of G- and P-Type Human Rotaviruses Circulating in South Korea

Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999–2002

Genetic variation of prevalent G1P[8] human rotaviruses in South Korea

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