Distribution of human papillomavirus among women with abnormal cervical cytology in Kuwait

Al‐Awadhi, Rana; Chehadeh, Wassim; Jaragh, Mohammad; Al-Shaheen, Azza; Sharma, Prem N.; Kapila, Kusum

doi:10.1002/dc.21778

Cited by 38 publications

(46 citation statements)

References 35 publications

(46 reference statements)

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“…The population-based type-specific prevalence of HPV 16 is ranging from around 2% to 4% in all regions [6]; however, HPV 16 infection is strongly associated with risk of cervical neoplasia and causes over 50% of cases of cervical cancer [4]. HPV 81 (equivalent to CP8304) is also reported to be associated with precancerous or cancerous lesions [18,19]. The prevalence of HPV 81 in worldwide population studies is not very high but HPV 81 is one of the most frequently observed HPV types in the HIV-positive patients [20,21].…”

Section: Resultsmentioning

confidence: 99%

“…Persistent infection with high-risk Alpha PVs is a prerequisite for the development of cervical cancer and its histological precursor, cervical intraepithelial lesions. Twelve HPVs (16,18,31,33,35,39,45, 51, 52, 56, 58, and 59) are classified by the International Agency for Research on Cancer as being carcinogenic to humans, with HPV 68 being recognized as probably carcinogenic. Several other HPV types (26,30,34,53,66,67,69,70,73,82,85, and 97) are possibly carcinogenic based on evolutionary similarity to the known cancer-causing types [3].…”

Section: Introductionmentioning

confidence: 99%

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HPV Prevalence and Detection of Rare HPV Genotypes in Hong Kong Women from Southern China with Cytological Abnormalities

Chu

Chow

et al. 2013

ISRN Virology

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Human papillomavirus (HPV) has been identified as the primary cause of cervical squamous intraepithelial lesion and invasive cervical cancer. The emergence of various commercial HPV genotyping kits with different characteristics facilitates the detection of most high-risk and low-risk HPV genotypes, but the rare HPV types are usually underdiagnosed. In the present study, HPV detection was performed using the GenoFlow HPV Array Test kit (DiagCor Bioscience), which can identify 33 HPV subtypes by specific probes. Besides, a HPV consensus probe (universal probe) was designed to capture not only the 33 genotypes but also rare subtypes. Of the 1643 Southern Chinese women tested between 2012 and 2013, the HPV prevalence was 42.3%, with HPV 52 (139/1643, 8.5%), HPV 81 (89/1643, 5.4%), and HPV 16 (63/1643, 3.8%) being the most frequent subtypes detected. Among all 695 HPV-positive cases, 56 (8.1%) cases were only detected by the universal probe, in which 5 were either ASCUS or LSIL cases. Sequencing results confirmed HPV types 30, 91, and 74, and the intratypic variants of HPV 72 and 82 were present in the 5 cases. The result suggests that some rare HPV subtypes might be involved in cervical lesions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HPV Prevalence and Detection of Rare HPV Genotypes in Hong Kong Women from Southern China with Cytological Abnormalities

Chu

Chow

et al. 2013

ISRN Virology

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“…However, in a study of 298 women with abnormal cervical cytology in Kuwait [27], reported that as the severity of the cytological diagnosis increased the proportion of single infections decreased, while the proportion of multiple infections increased, and Fife et al [39] found 85.3% of multiple HPV infections in dysplasia and 61.9% in ASC-US, these last concluding that their data support a possible role for multiple HPV types in the development or progression of cervical dysplasia. This is an issue that requires further investigation.…”

Section: Discussionmentioning

confidence: 97%

“…In all geographical regions, HPV prevalence was highest in women younger than 35 years of age, decreasing in older women [26]. Regarding age distribution, 69% of all HPV infections were found in women aged 20-29 years [27,28].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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BackgroundCervical cancer is the second most common cancer in women worldwide and the most common cause of mortality in underdeveloped and developing countries [1,2]. Persistent infection with certain oncogenic high-risk (HR) types of Human Papillomavirus (HPV) is the required factor for the development of invasive cervical cancer and precursor lesions [3][4][5][6].Overall, the prevalence and distribution of HPV infection in cervical lesions has been determined in many geographical regions of the world, this can vary depending on several factors including epidemiological differences in the populations studied and the methodology used for molecular detection and typing of HPV DNA [7]. Over 120 types of HPV have been classified as either low (LR-HPV) or high risk (HR-HPV), according to their oncogenic potential [8,9]. Within the high-risk genotypes, HPV types 16 and HPV 18 are most often associated with cancer and squamous intraepithelial lesion [5,6,10,11,12]. Nevertheless, the risk of neoplasia for other types of HPV as well as for multiple HPV infection has not yet been established.Because current strategies for the prevention of cervical cancer and their precancerous lesions are based on the HPV genotyping [13] and prophylactic vaccines [14][15][16], it seems necessary to determine the types of HPV most commonly associated with malignant cell transformation in different geographical areas in order to establish effective preventive measures according to the epidemiology of the population. This study was to know the prevalence and distribution of cervical HPV infection in women attending routine cervical cancer screening in the Valencia region (Spain). Study designLiquid-based cytology (LBC) of cervical specimens was collected during routine screening visits, between June 20, 2011 and September 9, 2011 in the Clinical Hospital of Valencia, Spain. LBC samples were processed using the Thin Prep® 5000 processor (Madrid, Spain). For HPV detection and genotyping, we used the residual cervical sample material available from the same Thin Prep Pap test vial previously used for cytological screening. DNA was extracted from the residual materials of LBC by QIAamp® DNA mini kit (IZASA, Valencia, Spain), according to the manufacturer's instructions. HPV testing was performed by INNO-Lipa HPV genotyping Extra Reverse Hybridization Line Probe Assay kit (Innogenetics®, Barcelona, Spain), according to the manufacturer's instructions. Briefly, HPV detection and genotyping was based on PCR amplification of a 65pb fragment, within the L1 region of the HPV genome, using broad-spectrum SPF10 biotinylated primers. PCR was performed in a final AbstractBackground: Infection with human papillomavirus (HPV) has been identified as the primary cause of cervical cancer. Persistent infection with high risk-HPV is the required factor for the development of cervical cancer.

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“…The assay was carried out on 3 different plates, as described previously [23,24]. The first plate was used to determine the integrity of the target DNA by β-globin PCR assay, amplifying a target of a 268-bp fragment as described previously by Lum and Le Marchand [25].…”

Section: Methodsmentioning

confidence: 99%

Immunocytochemical Detection of Raf Kinase Inhibitor Protein and Human Papillomavirus Profiling of Normal and Abnormal Cervical ThinPrep Samples

Al‐Awadhi

Husain

Chehadeh³

et al. 2013

Acta Cytologica

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Objectives: This study investigates the potential value of Raf kinase inhibitor protein (RKIP) as a marker of normal squamous cells in ThinPrep slides. RKIP was evaluated for its ability to distinguish between normal and abnormal cervical samples in the context of human papillomavirus (HPV) infections. Study Design: A total of 316 ThinPrep samples were taken from women with normal and abnormal cervices. ThinPrep slides were Papanicolaou stained and reported. Residual samples were used for RKIP immunostaining and HPV PCR-based sequencing. Results: RKIP expression was seen in both nuclei and cytoplasm in 83.7% of samples. RKIP expression was highest (84.6%) in samples with a diagnosis of high-grade squamous intraepithelial lesion (HSIL) or worse; expression was lower in low-grade squamous intraepithelial lesions (73%) and was lowest in samples with normal cytology (p = 0.0023). A total of 74% of HPV-infected ThinPrep samples were immunopositive, and 67% of samples that did not harbor HPV were also immunopositive (p = 0.414). Sensitivity and specificity of RKIP were 84.6 and 34.6%, respectively, for the detection of samples with HSIL or worse. Conclusions: This study showed that RKIP expression may be of some value as a marker for abnormal cervical cells. Combined RKIP expression and HPV testing could improve the identification of samples with abnormal cytology.

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Distribution of human papillomavirus among women with abnormal cervical cytology in Kuwait

Cited by 38 publications

References 35 publications

HPV Prevalence and Detection of Rare HPV Genotypes in Hong Kong Women from Southern China with Cytological Abnormalities

HPV Prevalence and Detection of Rare HPV Genotypes in Hong Kong Women from Southern China with Cytological Abnormalities

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Immunocytochemical Detection of Raf Kinase Inhibitor Protein and Human Papillomavirus Profiling of Normal and Abnormal Cervical ThinPrep Samples

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