Distribution of MICA diversity in the Chinese Han population by polymerase chain reaction sequence‐based typing for exons 2–6

Abstract

Major histocompatibility complex class I chain-related gene A (MICA) is located 46 kb centromeric to HLA-B locus and encodes a stress-inducible protein. MICA allelic variation is thought to be associated with disease susceptibility and immune response to transplants. In this study, polymerase chain reaction sequence-based typing (PCR-SBT) method for MICA alleles has been established. Genomic DNAs from 100 healthy Chinese Han individuals were typed for MICA alleles by this method. The microsatellite polymorphism in the exon 5 of MICA gene, MICA*Del allele, and human leukocyte antigen-B alleles was also detected by the polymerase chain reaction-GeneScan, polymerase chain reaction sequence-specific primer, and PCR-SBT methods, respectively. Fourteen MICA alleles were found in the population, with MICA*00801/04 having the highest frequency of 27.0%. MICA*A4, *A5, *A5.1, *A6, and *A9 microsatellites were identified. Two samples with HLA-B*4801 were MICA*Del positive, with the frequency of 1.0%. The data showed that the new PCR-SBT method for MICA alleles was reliable and Chinese Han population was distinct in distribution of MICA alleles.

Distribution of MICA haplotypes in a Chinese Han population

“…Similar MICA frequencies were observed among different Chinese ethnic groups; with the exception of MICA*019, which was higher in frequency (14.93%) in our cohort compared with other Chinese populations (3.85 [22] and 9.5% [23]). In the northern Chinese population, Tian and co-workers reported a lower frequency of MICA*12:01, MICA*019 and a higher frequency of MICA*004 [24].…”

“…In the northern Chinese population, Tian and co-workers reported a lower frequency of MICA*12:01, MICA*019 and a higher frequency of MICA*004 [24]. Deviations of MICA frequencies (MICA*045, -*027, and -*008:01) between our cohort and the population of Zhejiang Province in middle China [23] were observed.…”

Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region

“…To characterize the differences in MICA gene polymorphisms between the distinct ethnic groups, which may modify the susceptibility of different groups to schistosomiasis or affect associated pathologies, we compared the distribution of MICA alleles from the healthy controls in this study to those reported for Southern and Northern Han populations (22,23), as well as for Korean (24), Thai (25), American-Caucasian (23), and Afro-American populations (23). As shown in Table 4, no dramatic differences were observed in the MICA allelic distribution between the Han populations from different areas of China (P > 0.05).…”

MICA polymorphism in a northern Chinese Han population: The identification of a new MICA allele, MICA*059

“…The MICA polymorphism and its LD with HLA-B have been recently investigated for a southeastern Chinese Han population residing in Zhejiang Province [29], where MICA*045 was rarely found (1.5%) and significant LD of 6 A new MICA allele, MICA*059, was observed in this population. MICA*059 appeared to be carried on an HLA-B*1301 haplotype, suggesting that this new MICA allele is evolutionarily linked to and possibly derived from MICA*045 through a single mutation event.…”