Distribution of IgM and IgG antibodies to oxidized LDL in immune complexes isolated from patients with type 1 diabetes and its relationship with nephropathy

Virella, Gabriel; Carter, Rickey E.; Saad, Antonio F.; Crosswell, Edward G.; Game, Bryan A.; Dcct,; Lopes‐Virella, Maria F.

doi:10.1016/j.clim.2008.02.005

Cited by 40 publications

(35 citation statements)

References 49 publications

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“…78,7.03]; p=0.13). On the other hand, increases in IgM antibody concentration were not found to show a negative correlation with the development of nephropathy (OR=0.927 for 1 SD change; 95% CI [0.44, 1.96]; p=0.84) [83].…”

Section: Are Igm Antibodies To Modified Ldl Protective?mentioning

confidence: 83%

“…The circulating IC were precipitated from serum samples with 4% polyethylene glycol, resuspended, and fractionated in immobilized protein G columns. IgG and IgM oxLDL antibodies were assayed in the eluate and washout fractions of the Protein G column, respectively [83]. This approach has the advantage of measuring the specific antibodies of true significance, i.e., those able to form stable IC.…”

Section: Are Igm Antibodies To Modified Ldl Protective?mentioning

confidence: 99%

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Atherogenesis and the humoral immune response to modified lipoproteins

Virella¹,

Lopes‐Virella²

2008

Atherosclerosis

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Modified forms of LDL are immunogenic and activate both cell-mediated and humoral immune responses. Both types of responses are pro-inflammatory and are probably primary players in the perpetuation of the chronic inflammatory reaction characteristic of atherosclerosis. The immunologic response to modified LDL can be directed to MHC-II-associated peptides in the case of T helper cells, and to a variety of epitopes -modified lysine groups, modified phospholipids, proteins that become associated with oxidized LDL (such as β2GP1) -in the case of B cell responses. T cell activation is likely to play a major role through cross-activation of macrophages. Humoral responses to modified LDL are pathogenic as a consequence of the formation of antigen-antibody complexes containing modified LDL and IgG antibodies. Those immune complexes induce cholesterol ester accumulation in macrophages and macrophage-like cells, and induce the release of proinflammatory cytokines, chemokines, oxygen active radicals, and matrix metalloproteinases from those cells. There is no conclusive evidence supporting a protective role for IgM antibodies in humans, possibly because autoantibodies to modified lipoproteins are predominantly of the IgG isotype.

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Section: Are Igm Antibodies To Modified Ldl Protective?mentioning

confidence: 83%

Section: Are Igm Antibodies To Modified Ldl Protective?mentioning

confidence: 99%

Atherogenesis and the humoral immune response to modified lipoproteins

Virella¹,

Lopes‐Virella²

2008

Atherosclerosis

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“…It has been reported that lymphocytes from human atherosclerotic plaques recognize oxidized LDL (ox-LDL) in vitro [9]. The induction of autoantibodies against ox-LDL has also been reported in several other studies [10,11,12,13,14,15,16,17]. There are several epitopes of ox-LDL that induce autoimmune responses in humans.…”

Section: Introductionmentioning

confidence: 86%

Prooxidant-Antioxidant Balance and Antioxidized LDL Antibody Level Values and Cardiac Function in Patients with Coronary Artery Disease

Rahsepar

Mirzaee²,

Moodi³

et al. 2012

Cardiology

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Objectives: We studied the association between the prooxidant-antioxidant balance (PAB), anti-malondialdehyde-modified low-density lipoprotein (oxidized LDL, ox-LDL) IgG antibody and indices of cardiac function (systolic and diastolic function) in patients with coronary artery disease (CAD). Methods: Fifty-five patients with established CAD were selected, and serum levels of anti-ox-LDL IgG and PAB values were measured and compared with 40 matched healthy controls. Systolic and diastolic functions were determined for all patients. Results: PAB values were significantly higher in patients than in controls (p < 0.001), whilst serum anti-ox-LDL concentrations were not statistically different between the 2 groups (p = 0.821). However, after adjustment for high-density lipoprotein cholesterol, the patients had higher anti-ox-LDL levels (p = 0.04). Total PAB values were inversely associated with ejection fraction (r = –0.326, p = 0.031), but this was not the case for anti-ox-LDL in either group (p > 0.05). Conclusion: Serum concentrations of a marker of oxidative stress (PAB values) are inversely associated with cardiac function. PAB is a relatively simple index that could be incorporated into risk assessment in CAD patients. Anti-ox-LDL IgG antibody concentration does not appear to reflect total oxidative stress as assessed by PAB.

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“…The oxLDL particles bound to antibodies are taken up from the circulation via Fcγ II receptors present in cells from the mononuclear phagocytic system contributing to the inflammation in the vascular wall [11][12][13][14]. Involvement of these receptors in atherosclerosis has been recently demonstrated and decreased expression of FcγRII was found in patients with cardiovascular diseases [15].…”

Section: Introductionmentioning

confidence: 99%

Role of PPAR-gamma in the Modulation of CD36 and FcgammaRII induced by LDL with Low and High Degrees of Oxidation During the Differentiation of the Monocytic THP-1 Cell Line

Ríos¹,

Jancar²,

Melo³

et al. 2008

Cell Physiol Biochem

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Scavenger or Fcγ receptors are important for capture and clearance of modified LDL particles by monocytes/macrophages. Uptake via scavenger receptors is not regulated by intracellular levels of cholesterol and in consequence, macrophages develop into foam cells in the arterial intima. The levels of scavenger receptor CD36 are increased in atherosclerotic lesions and there is evidence that some components of oxLDL auto-regulate the expression of this receptor. Fcγ receptor expression is increased in cardiovascular diseases but it is not known weather their expression is regulated by oxLDL. The biological properties of oxLDLs vary depending on the degree of oxidation. In the present study we investigated the effect of LDL particles showing extensive or low oxidation (HoxLDL and LoxLDL) on the expression of CD36 and FcγRII in a human monocytic cell line (THP-1), differentiated or not to macrophage, and the involvement of PPARγ. It was found that both forms of oxLDL are able to increase the expression of CD36 and FcγRII and that this effect is dependent on the degree of oxidation and of the stage of cell differentiation (monocyte or macrophage). We also showed that the increased expression of FcγRII is dependent on PPARγ whereas that of the CD36 is independent of PPARγ.

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Distribution of IgM and IgG antibodies to oxidized LDL in immune complexes isolated from patients with type 1 diabetes and its relationship with nephropathy

Cited by 40 publications

References 49 publications

Atherogenesis and the humoral immune response to modified lipoproteins

Atherogenesis and the humoral immune response to modified lipoproteins

Prooxidant-Antioxidant Balance and Antioxidized LDL Antibody Level Values and Cardiac Function in Patients with Coronary Artery Disease

Role of PPAR-gamma in the Modulation of CD36 and FcgammaRII induced by LDL with Low and High Degrees of Oxidation During the Differentiation of the Monocytic THP-1 Cell Line

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