Distribution of Inclusion Bodies in Tissues from 100 Dogs Infected with Canine Distemper Virus

Kubo, Takuya; Kagawa, Yumiko; Taniyama, Hiroyuki; Hasegawa, Atsuhiko

doi:10.1292/jvms.69.527

Cited by 12 publications

(13 citation statements)

References 6 publications

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“…geralmente é relacionada ao tratamento com antimicrobianos que destroem a flora microbiana normal e à imunodepressão ocasionada pela infecção viral (Gelberg 2007). Kubo et al (2007) encontrou inclusões virais na bexiga de caninos infectados pelo vírus da cinomose em 73,0% dos casos. Esse dado difere do encontrado no presente estudo onde, apenas 27,8% dos cães apresentaram corpúsculos de inclusão na bexiga.…”

Section: Discussionunclassified

Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina

et al. 2009

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RESUMO.-A cinomose canina é uma doença viral e afeta principalmente os sistemas respiratório, gastrintestinal e nervoso. Neste trabalho foram analisados os achados patológicos e imuno-histoquímicos de 54 cães com cinomose de um total de 760 cães necropsiados no período de julho de 2006 a outubro de 2007. As lesões macroscó-picas observadas foram caracterizadas por corrimento ocular e nasal mucopurulentas, hiperqueratose dos coxins digitais, pulmões avermelhados e não colapsados, atrofia do timo, conteúdo intestinal diarréico e placas de Peyer proeminentes. Os achados histológicos caracterizavamse principalmente por pneumonia intersticial, rarefação linfóide, desmielinização da substância branca, manguitos perivasculares e corpúsculos de inclusão intranucleares e intracitoplasmáticos, que se localizam principalmente na mucosa do estômago, epitélios da bexiga, brônquios e bronquíolos, pelve renal, coxins digitais, pálpebra, orelha e tonsila no sistema nervoso central e em células mononucleares dos linfonodos, baço e tonsilas. Os tecidos foram marcados pela técnica imuno-histoquímica utilizan- Canine distemper is a viral disease that affects mainly respiratory, gastrointestinal and nervous system. The present study analyzes pathologic and immunohistochemical findings in 54 dogs with canine distemper of a total of 760 canine necropsies performed from July 2006 to October 2007. The gross lesions were characterized by mucopurulent oculonasal discharge, hyperkeratosis of footpads, red and not collapsed lungs, thymic atrophy, watery intestinal content, hyperemia and enlarged Peyer's patches. The histological findings were characterized by interstitial pneumonia, lymphoid depletion, white matter demyelization, perivascular cuffs and intracytoplasmatic and intranuclear inclusion bodies located in epithelial cells of gastric mucosa, urinary bladder, bronchial, renal pelvis, footpads, eyelid, skin of the ear, tonsil, central nervous system and mononuclear cells in lymph nodes, spleen, and tonsils. Viral antigen was detected by an immunohistochemical procedure using a mouse monoclonal anti-canine distemper antibody. The footpads were the more constantly (67.4% of the cases) immunolabeled tissue, followed by stomach with 62.7%. Immunohistochemistry was demonstrated to be a useful tool for the study of viral antigen distribution in distemper affected dogs as well as it indicated which is the best tissue to be examined in order to confirm a suspected case of canine.INDEX TERMS: Canine diseases, distemper, immunohistochemistry.

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Section: Discussionunclassified

Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina

et al. 2009

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“…We did not test urine samples; however, in a recent study, urine has been described as a sensitive sample for the detection of CDV in live dogs (2). Postmortem samples were from tonsils, brains, bladders, and lungs (16). Approximately 2 to 5 g of each tissue was received in tubes sent on ice by overnight delivery for virological examination.…”

Section: Canine Distemper Virus (Cdv) Is a Morbillivirus That Affectsmentioning

confidence: 99%

Canine Distemper Virus Strains Circulating among North American Dogs

Kapil¹,

Allison²,

Johnston³

et al. 2008

Clin Vaccine Immunol

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Canine distemper virus (CDV) is a highly contagious virus that causes multisystemic disease in dogs. We received seven samples from dogs with CD from the United States during 2007. CDV isolates from these samples formed large, multinucleated syncytia in a Vero cell line expressing canine signaling lymphocyte activation molecule (SLAM). Based on the hemagglutinin gene sequences, the CDV isolates from three states (California, Missouri, and Oklahoma) formed two CDV genetic groups: group I (major; six of seven isolates) consisted of CDV isolates closely related to the European wildlife lineage of CDV, and group II (minor; one of seven isolates) was genetically related to the Arctic-like lineage of CDV. However, both CDV groups were genetically different from the current vaccine strains that belong to the American-1 lineage of the old (1930 to 1950) CDV isolates.

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“…Pancreatic lesions were frequent in this study and Tams (2005) cites that the canine distemper virus can cause pancreatitis, reinforcing the findings of this work. Lymphoid atrophy was observed in 29.4% of dogs as reported by Kubo et al (2007). Deem et al (2000) cited pneumonia in dogs with distemper as one of the important postmortem findings and in their study 52.92% of the dogs had pneumonia.…”

Section: Discussionmentioning

confidence: 57%

“…Virus inclusions bodies were identified in the bladder epithelial cells in 41.18% of the animals, and it may be considered an important microscopic finding for the diagnosis of distemper. Kubo et al (2007) observed viral inclusions bodies in the bladder of canines infected with the distemper virus in 73.0% of the cases. Sonne et al (2009) in 27.8% of the dogs and these authors associated the low frequency of inclusions in the bladder with the desquamation observed in the epithelium of the same making it impossible to visualize the inclusion bodies.…”

Section: Discussionmentioning

confidence: 90%

“…Deem et al (2000) cited pneumonia in dogs with distemper as one of the important postmortem findings and in their study 52.92% of the dogs had pneumonia. Sonne et al (2009) verified 44.4% of the animals with pneumonia and Kubo et al (2007) reported that the main finding in 100 dogs with distemper was pneumonia. Also Tudury et al (1997) reported 12.34% of dogs with distemper showing bronchopneumonia.…”

Section: Discussionmentioning

confidence: 97%

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Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes

et al. 2020

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Canine distemper is a viral disease that affects several systems on dogs, among them, the cardiovascular system. The aim of this study was to identify canine distemper virus (CDV) in the sinoatrial node (SAN) of dogs serologically positive for distemper by Polymerase Chain Reaction preceded by reverse transcription (RT-PCR), and to analyze gross and microscopic changes of distemper in the heart and other tissues. SAN and tissue fragments were collected from 17 serologically positive dead animals, necropsied from October 2015 to December 2016. In the heart, right heart dilatation was observed in 13 dogs (76.47%) and left concentric hypertrophy in two dogs (11.76%). Microscopically, lymphocytic myocarditis was observed in four (23.53%) dogs and 41.18% presented viral inclusion corpuscles of CDV in the bladder epithelium. Only one (5.88%) dog presented a 319 bp target fragment for distemper virus using primers CDV 1 and CDV 2 at the sinoatrial node. In conclusion, CDV can be located in the sinoatrial node of naturally infected dogs, as demonstrated in this study by the RT-PCR technique, reinforcing the hypothesis that CDV is capable of causing inflammatory lesions in the sinoatrial node of this species. Macroscopic and microscopic cardiac changes are frequently observed in dogs with distemper, mainly cardiac dilatation and myocarditis. Viral inclusions of CDV in bladder epithelial cells are an important microscopic finding for the diagnosis of distemper.

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Distribution of Inclusion Bodies in Tissues from 100 Dogs Infected with Canine Distemper Virus

Cited by 12 publications

References 6 publications

Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina

Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina

Canine Distemper Virus Strains Circulating among North American Dogs

Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes

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