Distribution of intravenously and perorally administeredAeromonas salmonicidalipopolysaccharide in Atlantic salmon,Salmo salarL.

Distribution of intravenously injected A-layer protein and lipopolysaccharide (LPS) from Aeromonas salmonicida in Atlantic salmon, Salmo salar L.

“…2 as administration of FITC-LPS resulted in a high specific uptake in both spleen and head kidney whereas TC-LPS was trapped in the kidneys. Our results based on FITC-conjugated LPS are in accordance with a recent report (Dalmo & Bøgwald, 1996) revealing a high uptake of radiolabelled LPS ( 3 H-LPS) in spleen, posterior and anterior kidneys after i.v. injection.…”

“…LPS was isolated from A. salmonicida according to the method of Westphal & Jann (1965), with some modifications (Dalmo & Bøgwald, 1996). The content of neutral hexoses was quantified according to Dubois et al (1956) and Fox & Robyt (1991).…”

“…Previously, LPS has been reported to locate both in splenic ellipsoids and in cells in the head kidney of salmon (Dalmo & Bøgwald, 1996;Espenes et al, 1996), and also in the heart of a marine fish species (Atlantic cod) (Dalmo et al, 1998). In salmon vaccinated against furunculosis, the A-layer protein has been detected in melanomacrophages of the head kidney and in a small population of tissue macrophages located near melanomacrophages in the spleen .…”

The morphology of the immune system in teleost fishes

“…Recent investigations in brown trout, Salmo trutta L., and in Atlantic salmon have shown that the anterior intestine is also able to 314 C. McL. PRESS AND Ø. EVENSEN absorb macromolecules and deliver the absorbed substance to the circulation (O'Donnell et al, 1994;Dalmo & Bøgwald, 1996). The whole salmonid intestine would thus appear capable of absorbing macromolecules.…”

Immunoglobulin genes and antibody responses in the spotted wolffish (Anarhichas minor Olafsen)

“…In addition to whole bacterial cells, purified preparations of A. salmonicida A-layer protein (Bjørnsdottir et al, 1992) and LPS (Dalmo & Bøgwald, 1996) were coated on to separate microtitre plates. Individual antisera or pools of sera were tested against the various antigens using secondary rabbit anti-wol$sh Ig antibodies, followed by alkaline phosphatase-conjugated swine anti-rabbit Ig antiserum and the substrate p-nitrophenyl phosphate (Sigma) to develop a coloured reaction measured at O.D.…”