Distribution of lignin in normal and compression wood of Pinus taeda L.

Parham, Russell A.; CôtéJr., W. A.

doi:10.1007/bf00363120

Cited by 41 publications

(12 citation statements)

References 7 publications

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“…In compression wood, the lignin distribution pattern differed from those described in other studies (Parham, C6te 1971;Wood, Goring 1971 ;Timell 1982). Reductions in cell wall layers typically high in lignin, such as the compound middle lamella tissue and the $1, were evident.…”

Section: Discussioncontrasting

confidence: 48%

Lignin distribution across tracheid cell walls of poorly lignified wood from deformed copper deficient Pinus radiata (D. Don)

1991

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Lignin topochemistry of tracheid walls from a deformed, copper deficient Pinus radiata (D. Don) tree was examined by linescan and point analyses using a Scanning Electron Microscope and Energy Dispersive Spectrometry. Both opposite and compression wood had abnormal lignin distributions compared to those observed in normal wood from a straight tree. Lignin contents in the compound middle lamella were lower than lignin contents in the secondary wall in both opposite and compression wood tracheids.

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Section: Discussioncontrasting

confidence: 48%

Lignin distribution across tracheid cell walls of poorly lignified wood from deformed copper deficient Pinus radiata (D. Don)

1991

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“…It was therefore of interest to re-assess lignification in tension wood with novel approaches having higher sensitivity and specificity than the previously used techniques. Former studies of lignin in tension wood relied either on highly drastic methods such as the lignin skeleton method using hydrofluoric acid (Parham and Coˆte´1971) or staining methods such as KMnO 4 (Blanchette et al 1994). Both techniques lack the sensitivity allowing detection of discrete amounts of lignin amid a large amount of polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Detection in situ and characterization of lignin in the G -layer of tension wood fibres of Populus deltoides

Joseleau

Imai

Kuroda

et al. 2004

Planta

135

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The occurrence of lignin in the additional gelatinous (G-) layer that differentiates in the secondary wall of hardwoods during tension wood formation has long been debated. In the present work, the ultrastructural distribution of lignin in the cell walls of normal and tension wood fibres from poplar (Populus deltoides Bartr. ex Marshall) was investigated by transmission electron microscopy using cryo-fixation-freeze-substitution in association with immunogold probes directed against typical structural motifs of lignin. The specificity of the immunological probes for condensed and non-condensed guaiacyl and syringyl interunit linkages of lignin, and their high sensitivity, allowed detection of lignin epitopes of definite chemical structures in the G-layer of tension wood fibres. Semi-quantitative distribution of the corresponding epitopes revealed the abundance of syringyl units in the G-layer. Predominating non-condensed lignin sub-structures appeared to be embedded in the crystalline cellulose matrix prevailing in the G-layer. The endwise mode of polymerization that is known to lead to these types of lignin structures appears consistent with such an organized cellulose environment. Immunochemical labelling provides the first visualization in planta of lignin structures within the G-layer of tension wood. The patterns of distribution of syringyl epitopes indicate that syringyl lignin is deposited more intensely in the later phase of fibre secondary wall assembly. The data also illustrate that syringyl lignin synthesis in tension wood fibres is under specific spatial and temporal regulation targeted differentially throughout cell wall layers.

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“…There are no specific measurements for the S1 layer because of its small size but transmission electron microscopy using permanganate staining often indicates that the S1 layer is slightly less lignified than the S2 layer (Donaldson, 1995;Maurer and Fengel, 1991). The S3 layer is variable but is generally considered to be more lignified than the S2 layer with measurements of around 50% lignin w/w (Bland et al, 1971;Donaldson, 1987;Parham and Cô té, 1971;Scott and Goring, 1970;Timell, 1973).…”

Section: B Lignin Distributionmentioning

confidence: 98%