Distribution of &lt;i&gt;vip&lt;/i&gt; Genes, Protein Profiling and Determination of Entomopathogenic Potential of Local Isolates of &lt;i&gt;Bacillus thuringiensis&lt;/i&gt;

Shingote, Prashant Raghunath; Moharil, M. P.; Dhumale, Dipti R.; Deshmukh, A. G.; Jadhav, P. V.; Dudhare, M. S.; Satpute, N. S.

doi:10.5376/bt.2013.04.0003

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Vip3Aa45 was found more toxic against Mamestra brassicae and Vip3Ag4 against Chrysodeixis chalcites (Palma et al, 2013). The protein expressed from partial vip3-type genes (1113 bp) identified in isolates PDKV-08, PDKV-21, NCIM-5110, and NCIM-5132 exhibited lower toxicity than the reference strain HD-1 against H. armigera (Shingote et al, 2013). The vip3-gene from Bt supsp.…”

Section: Cloning Characterization and Toxicity Potential Of Vip3-genesmentioning

confidence: 99%

Vegetative Insecticidal Protein (Vip): A Potential Contender From Bacillus thuringiensis for Efficient Management of Various Detrimental Agricultural Pests

2021

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Bacillus thuringiensis (Bt) bacterium is found in various ecological habitats, and has natural entomo-pesticidal properties, due to the production of crystalline and soluble proteins during different growth phases. In addition to Cry and Cyt proteins, this bacterium also produces Vegetative insecticidal protein (Vip) during its vegetative growth phase, which is considered an excellent toxic candidate because of the difference in sequence homology and receptor sites from Cry proteins. Vip proteins are referred as second-generation insecticidal proteins, which can be used either alone or in complementarity with Cry proteins for the management of various detrimental pests. Among these Vip proteins, Vip1 and Vip2 act as binary toxins and have toxicity toward pests belonging to Hemiptera and Coleoptera orders, whereas the most important Vip3 proteins have insecticidal activity against Lepidopteran pests. These Vip3 proteins are similar to Cry proteins in terms of toxicity potential against susceptible insects. They are reported to be toxic toward pests, which can’t be controlled with Cry proteins. The Vip3 proteins have been successfully pyramided along with Cry proteins in transgenic rice, corn, and cotton to combat resistant pest populations. This review provides detailed information about the history and importance of Vip proteins, their types, structure, newly identified specific receptors, and action mechanism of this specific class of proteins. Various studies conducted on Vip proteins all over the world and the current status have been discussed. This review will give insights into the significance of Vip proteins as alternative promising candidate toxic proteins from Bt for the management of pests in most sustainable manner.

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Section: Cloning Characterization and Toxicity Potential Of Vip3-genesmentioning

confidence: 99%

Vegetative Insecticidal Protein (Vip): A Potential Contender From Bacillus thuringiensis for Efficient Management of Various Detrimental Agricultural Pests

2021

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“…The incorporation of genetic material into plants renders them unsuitable for pest attack. The best-known insecticidal molecules used in PIP technology are Cry proteins from the soil species of Bacillus thuringiensis, protease from Baculovirus, toxic complex (Tc) proteins from bacteria of the genera Xenorhabdus and Photorhabdus, as well as double-stranded ribonucleic acid (dsRNA) and Mir1-CP from maize (Fenibo et al, 2021;Parker & Sander, 2017;Shingote et al, 2013;Wei et al, 2018).…”

Section: Integrated Agricultural Systemsmentioning

confidence: 99%

Sustainable food production and processing—sustainable agriculture and biotechnological approaches in food chain

Gwiazdowska,

Marchwińska,

Juś

2024

Sustainable Food. Production and Consumption Perspectives

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The current trend of the increasing human population as well as the evolution of consumption patterns, increasing food demand and growing amounts of food waste influence changes along the entire food chain, from agricultural systems and natural resources to processing. It is worth underlining that the agri-food industry is considered one of the most important sectors of economic development in the world. However, the increased demand for food is depleting natural resources, causing soil erosion, landscape biodiversity loss and environmental pollution worldwide, creating new challenges for food security and sustainable food production. Therefore, sustainable agriculture and new technologies and approaches play an increasingly significant role in reducing negative environmental impacts while ensuring food safety. It stays in line with the model of food production development promoted by the Food and Agriculture Organization of the United Nations, according to which sustainable agriculture means the production of healthy, high-quality food in an environmentally friendly way, caring for animal welfare and protecting biodiversity, as well as ensuring income for farmers. This approach is also consistent with many concepts focused on the issue of sustainable, eco-friendly food production, such as development of sustainable agriculture, the One Health concept, Climate-Smart Agriculture, the European Green Deal and the Farm to Fork Strategy, strongly emphasising efforts to create a healthier and more sustainable food system.

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“…Transgenic plants (PIPs) offer an attractive alternative. They contain molecules such as Bt Cry proteins, α-amylase inhibitors, or double-stranded ribonucleic acid (dsRNA), which deter insect pests from targeting these plants [68][69][70].…”

Section: Bioinsecticidesmentioning

confidence: 99%

Diverse Approaches to Insect Control: Utilizing Brassica carinata (A.) Braun and Camelina sativa (L.) Crantz Oil as Modern Bioinsecticides

Rzyska,

Stuper-Szablewska,

Kurasiak-Popowska

2024

Forests

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The forest environment is exposed to a number of harmful factors that significantly reduce the resistance of forest stands, often leading to their extinction. In addition to abiotic and anthropogenic factors, biotic factors pose a significant threat to forests, among which insect pests are at the top of the list. Until now, the use of chemical insecticides has been considered the most effective method of pest control, resulting in pesticide residue in the environment. In an effort to minimize the harmful effects of insecticides, the European Union (EU), through EU Commission Implementing Regulations 2022/94, 2021/2081, 2021/795, and 2020/1643, has decided to withdraw from use a number of preparations containing compounds such as phosmet, indoxacarb, alpha-cypermethrin, and imidacloprid, among others. Botanical insecticides appear to be a promising alternative. Among them, plant oils and essential oils have become an innovative solution for controlling pests not only of forests but also of agricultural crops. The purpose of this literature review was to select oilseed plants with great biological potential. The rich chemical compositions of the seeds of Brassica carinata (A.) Braun and Camelina sativa (L.) Cranz predispose them to use as raw materials for the production of biopesticides with broad mechanisms of action. On the one hand, the oil will provide a physical action of covering pests feeding on a plant with a thin film, which will consequently lead to a reduction in gas exchange processes. On the other hand, the bioactive compounds in it or extracts of fat-insoluble compounds suspended in it and derived from the pomace fraction may have deterrent or lethal effects. This paper presents evidence of the potential action of these raw materials. Preparations based on these oils will not pose a threat to living beings and will not negatively affect the environment, thus allowing them to gain social acceptance.

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Distribution of <i>vip</i> Genes, Protein Profiling and Determination of Entomopathogenic Potential of Local Isolates of <i>Bacillus thuringiensis</i>

Cited by 5 publications

References 19 publications

Vegetative Insecticidal Protein (Vip): A Potential Contender From Bacillus thuringiensis for Efficient Management of Various Detrimental Agricultural Pests

Vegetative Insecticidal Protein (Vip): A Potential Contender From Bacillus thuringiensis for Efficient Management of Various Detrimental Agricultural Pests

Sustainable food production and processing—sustainable agriculture and biotechnological approaches in food chain

Diverse Approaches to Insect Control: Utilizing Brassica carinata (A.) Braun and Camelina sativa (L.) Crantz Oil as Modern Bioinsecticides

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