Distribution of ¹²⁵I-Prolactin in Mice and Rats. Studies with Whole-Body and Microautoradiography

Abstract

The distribution of ¹²⁵I-labeled ovine prolactin was studied in mice and rats after an intravenous injection by using whole-body and microautoradiography. In the whole-body autoradiography the labeled hormone rapidly accumulated in liver and kidney, but additionally some labeling was noted in the ventral prostate, seminal vesicles and mammary glands. In microautoradiography the grains were localized almost exclusively in the proximal tubular cells of the kidney. In the liver the labeling was more diffuse over both the parenhymal and the KupfTer cells. In the ovary the labeling was weak and mainly localized over the corpus luteum. Only minor differences were encountered in the intensity of labeling during the sexual cycle, pregnancy and lactation. The mammary epithelium showed some labeling during pregnancy and lactation. In the testis the grains were localized around the Leydig cells with no indication of tubular distribution. The ventral prostate showed a clear labeling of the epithelium. With increasing time after the injections the grains were rapidly localized toward the prostatic acinar lumen. Only a weak labeling of the preputial glands and seminal vesicles was observed. The role of prolactin as a synergist in the hormonal control of a variety of target organs was discussed.

Effects of induced hypoprolactinaemia in the ram : plasma gonadotrophin levels, LH and FSH receptors and histology of the testis

“…3c, d) A stimulating effect of bromocryptine treatment on FSH levels has also been reported in suckling ewes (Kann et al, 1977) and women (Seki et al, 1974 ;Nader et al, 1975). However, our results contrast with the increased release of FSH in hyperprolactinaemic rats (Voogt et al, 1969), dwarf mice (Bartke et al, 1977) and hamsters and also with the decreased secretion of FSH in men treated with bromocryptine (Lackritz and Bartke, 1980 (Rajaniemi et al, 1974 ;Charreau et al, 1977), as well as bromocryptine per se (Vermes and Telegdy, 1978) and FSH (Ketelslegers et a/., 1978) As suggested earlier (Barenton and Pelletier, 1980 ;Barenton, 1981) (Stolla et al, 1978) and mice (Bartke, 1974 …”

Prolactin-Induced Stimulation of Rat Renal Adenylate Cyclase and Autoradiographic Localization to the Distal Nephron

“…The present results are in conflict with the work of Rajaniemiet al [14] and Donatsch and Richardson [15]. Rajaniemi el al.…”

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

“…This was confirmed with the immunoperoxidase technique. Although these findings have not been reported for PTH, localization of insulin (33)(34)(35), glucagon (36), growth hormone (37)(38)(39), luteinizing hormone, and follicle-stimulating hormone (40,41), human chorionic gonadotropin (42), and prolactin (43) (13), and our current data-showing that localization of 125I-labeled and unlabeled preparations are identical, indicate that the metabolism ofthe radioiodinated PTH used in these studies accurately reflects the metabolism of unlabeled hormone.…”