Distribution of Lubricin in the Ruptured Human Rotator Cuff and Biceps Tendon: A Pilot Study

Funakoshi, Tadanao; Martin, Scott D.; Schmid, Thomas; Spector, Myron

doi:10.1007/s11999-009-1108-z

Cited by 15 publications

(13 citation statements)

References 32 publications

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“…The results of this study and prior work on the ruptured human supraspinatus and biceps tendons 17 indicate that lubricin-binding molecules are…”

Section: Discussionmentioning

confidence: 51%

“…Our sample size power analysis was based on a previous study of ruptured rotator cuff tendons. 17 The principal outcome variable was the presence or absence of a lubricin layer on the torn edge of the tissue. The transected edge produced during tissue excision, at least 2 mm away from the torn edge, served as the internal control.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemical localization of lubricin within cells in the bovine knee compartment 14 suggests local synthesis of this glycoprotein. The previous finding of lubricin on the ruptured surface of human supraspinatus and biceps tendons 17 raises questions regarding the distribution of lubricin within the human torn ACL and meniscus and its effects on healing.…”

mentioning

confidence: 99%

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Lubricin distribution in the torn human anterior cruciate ligament and meniscus

Zhang

Cheriyan

Martin

et al. 2011

Journal Orthopaedic Research

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The objective of this study was to: (1) determine the distribution of lubricin in the human torn anterior cruciate ligament (ACL) and meniscus; (2) determine the distribution of lubricin in the human intact ACL and meniscus; (3) and identify potential cellular sources of lubricin in these tissues. Ten torn ACLs and six torn menisci were obtained from surgeries; for comparison, 11 intact ACLs and 13 intact menisci were obtained from total knee replacements. Samples were formalin fixed and processed for immunohistochemical staining with a monoclonal antibody for lubricin. In torn ACLs and menisci, lubricin was generally found as a discrete layer covering the torn surface. No surface lubricin staining was found on the transected edges produced during excision. Lubricin was also found on the native surfaces of intact ACLs and menisci. In all tissues, lubricin was found in the matrix and intracellularly.

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“…The results of this study and prior work on the ruptured human supraspinatus and biceps tendons 17 indicate that lubricin-binding molecules are…”

Section: Discussionmentioning

confidence: 51%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Lubricin distribution in the torn human anterior cruciate ligament and meniscus

Zhang

Cheriyan

Martin

et al. 2011

Journal Orthopaedic Research

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“…The Proteoglycan 4 ( Prg4 ) gene, which encodes for lubricin among other proteins, may have a role in fascicle gliding [14] in tendon, but is also strongly expressed in the synovium of the joint capsule and in articular cartilage [15]. While lubricin expression has been shown during healing of the supraspinatus tendon [16], the contribution of Prg4-expressing cells to the healing response, particularly from the tendon fascicle or underlying synovium, remains largely unknown. In addition, rotator cuff tendon injuries typically occur near the fibrocartilage of the enthesis and the ability of these cells to contribute to the injury response has not been fully studied.…”

Section: Introductionmentioning

confidence: 99%

Murine supraspinatus tendon injury model to identify the cellular origins of rotator cuff healing

Yoshida

Alaee

Dyrna

et al. 2016

Connective Tissue Research

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Purpose of this study To elucidate the origin of cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where a full-thickness, central detachment is created in the supraspinatus. Materials and Methods Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, and AGC-9) were used to label different cell populations in the shoulder. The defect was created surgically in the supraspinatus. The mice were injected with tamoxifen at surgery to label the cells and sacrificed at 1, 2, and 5 weeks post-operatively. Frozen sections were fluorescently imaged then stained with Toluidine Blue and re-imaged. Results Three notable changes were apparent post-operatively. 1) A long thin layer of tissue formed on the bursal side overlying the supraspinatus tendon. 2) The tendon proximal to the defect initially became hypercellular and disorganized. 3) The distal stump at the insertion underwent minimal remodeling. In the uninjured shoulder, tdTomato expression was seen in the tendon midsubstance and paratenon cell on the bursal side in PRG4-9, in paratenon, blood vessels, and periosteum of acromion in SMA-9, and in articular cartilage, unmineralized fibrocartilage of supraspinatus enthesis, and acromioclavicular joint in AGC-9 mice. In the injured PRG4-9 and SMA-9 mice, the healing tissues contained an abundant number of tdTomato+ cells, while minimal contribution of tdTomato+ cells was seen in AGC-9 mice. Conclusions The study supports the importance of the bursal side of the tendon to rotator cuff healing and PRG4 and αSMA may be markers for these progenitor cells.

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“…It has been proposed that lubricin facilitates microscale lubrication between collagen fibrils when the surface is subjected to compression and shear. This hypothesis is supported by the selective localization of lubricin in other areas of elevated shear and tensile stresses: the collagen bundles throughout the entire knee meniscus, the tendon fascicles in digital flexor tendons, and the rotator cuff (Funakoshi et al, 2010; Sun et al, 2006; Zhang et al, 2011). …”

Section: Introductionmentioning

confidence: 92%

Lubricin deficiency in the murine lumbar intervertebral disc results in elevated torsional apparent modulus

Teeple

Aslani

Shalvoy

et al. 2015

Journal of Biomechanics

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The purpose of this study was to investigate the mechanical consequences of proteoglycan 4 (Prg4) deficiency on intervertebral disc mechanics using a Prg4 knockout mouse model. Prg4, also called lubricin, was first identified as the boundary lubricant in synovial fluid but has subsequently been localized within a number of musculoskeletal tissues in areas subjected to shear and tensile stresses, including the intervertebral disc. The function of lubricin in the intervertebral disc has not been determined. Lumbar level 1–2 vertebral body-disc-vertebral body motion segments were isolated from Prg4 null mice and wild type (WT) litter mate controls. Disc dimensions were measured and motion segments were tested in axial loading and torsion. Torque measurements and disc dimensions were used to calculate the torsional apparent modulus for discs from Prg4 null and WT discs. Discs from Prg4 null mice had a significantly smaller mean transverse disc area (p=0.0057), with a significantly larger proportion of this area occupied by the nucleus pulposus (p<0.0001), compared to WT specimens. Apparent torsional moduli were found to be elevated in Prg4 null lumbar discs compared to WT controls at 10–10° (p=.0048) and 10–30° (p=0.0127) rotation. This study suggests a functional role for Prg4 in the murine intervertebral disc. The absence of Prg4 was associated with an increased apparent torsional modulus and the structural consequences of Prg4 deficiency in the intervertebral disc, with expansion of the area of the nucleus pulposus relative to the transverse disc area in Prg4 null specimens.

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Distribution of Lubricin in the Ruptured Human Rotator Cuff and Biceps Tendon: A Pilot Study

Abstract: Background Lubricin is a lubricant for diarthrodial joint tissues and has antiadhesion properties; its presence in the (caprine) rotator cuff suggests it may have a role in intrafascicular lubrication.

Cited by 15 publications

References 32 publications

Lubricin distribution in the torn human anterior cruciate ligament and meniscus

Lubricin distribution in the torn human anterior cruciate ligament and meniscus

Murine supraspinatus tendon injury model to identify the cellular origins of rotator cuff healing

Lubricin deficiency in the murine lumbar intervertebral disc results in elevated torsional apparent modulus

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