Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil

Queiroz, Casley Borges de; Miranda, Edma Carvalho de; Hanada, Rogério Eiji; Sousa, N. R.; Gasparotto, L.; Soares, Marcos Antônio; Silva, G F

doi:10.4238/2013.february.8.9

Cited by 8 publications

(9 citation statements)

References 35 publications

(38 reference statements)

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“…Recent data on the distribution of mating types in the M. fijiensis population of Brazil demonstrate the occurrence of sexual recombination, which should increase genetic diversity (Queiroz et al, 2013). However, the data obtained in our study indicate a decrease in genetic and genotypic diversity among subpopulations of M. fijiensis from different geographical origins in Brazil.…”

Section: Discussioncontrasting

confidence: 55%

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

Silva¹,

Paixão²,

Queiroz³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The Enterobacterial repetitive intergenic consensus (ERIC)marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection sites in Brazil. A total of 9 loci were amplified, 77.8% of which were found to be polymorphic. The genetic diversity found in the population was 0.20. Analysis of molecular variance (AMOVA) demonstrated that the highest level of genetic variation is within populations. Cluster analysis revealed three main groups in Brazil, with no correlation between geographic and genetic distance.

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Section: Discussioncontrasting

confidence: 55%

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

Silva¹,

Paixão²,

Queiroz³

et al. 2014

Genet. Mol. Res.

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“…This might suggest the presence of P. fijiensis pathotypes capable of overcoming resistance in Yangambi KM5. Isolates belonging to mating type 1 and 2 of P. fijiensis have been observed under field conditions and occurring in equal ratios in Mexico and Brazil (Conde Ferráez et al , ; Queiroz et al , ). Similar observations were made in this study (data not presented), pointing to the possibility of frequent sexual reproduction in nature.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Pseudocercospora species causing Sigatoka leaf diseases of banana in Uganda and Tanzania

et al. 2019

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Sigatoka leaf diseases are a major constraint to banana production. A survey was conducted in Tanzania and Uganda to assess the distribution of Pseudocercospora species and severity of Sigatoka leaf diseases. Pseudocercospora species were identified using species‐specific primers. Sigatoka‐like leaf diseases were observed in all farms and on all cultivars, but disease severity varied significantly (P < 0.001) between countries, districts/regions within countries, altitudinal ranges and banana cultivars. In all regions except Kilimanjaro, P. fijiensis, the causal agent of black Sigatoka, was the only pathogen associated with Sigatoka disease. Mycosphaerella musae was associated with Sigatoka‐like symptoms in Kilimanjaro region. Black Sigatoka disease was more severe in Uganda, with a mean disease severity index (DSI) of 37.5%, than in Tanzania (DSI = 19.9%). In Uganda, black Sigatoka disease was equally severe in Luwero district (mean DSI = 40.4%) and Mbarara district (mean DSI = 37.9%). In Tanzania, black Sigatoka was most severe in Kagera region (mean DSI = 29.2%) and least in Mbeya region (mean DSI = 11.5%). Pseudocercospora fijiensis, the most devastating sigatoka pathogen, was detected at altitudes of up to 1877 m a.s.l. This range expansion of P. fijiensis, previously confined to altitudes lower than 1350 m a.s.l. in East Africa, is of concern, especially for smallholder banana farmers growing the susceptible East African Highland bananas (EAHB). Among the banana varieties sampled, the EAHB, FHIA hybrids and Mchare were the most susceptible. Here, the loss of resistance in Yangambi KM5, a banana variety previously resistant to P. fijiensis, is reported for the first time.

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“…Mating type idiomorph-harboring genome regions appear to be conserved among closely related species in the genus (Arzanlou et al, 2010;Conde-Ferráez et al, 2010;Kim et al, 2013). For P. fijiensis (Zhan et al, 2002;Conde-Ferráez et al, 2010;Queiroz et al, 2013) and Zymoseptoria tritici (Gurung et al, 2011), analysis of the mating type region has often revealed a frequency close to 1:1 of MAT1-1 and MAT1-2, indicating regular cycles of sexual recombination during the crop growing season. Unlike these Dothideomycete fungal species, analysis of the distribution of mating type idiomorphs in P. musae populations has so far been limited (Gomes et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Azole Fungicide Sensitivity in Pseudocercospora musae Field Populations in Brazil

et al. 2020

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Pseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel's test (r = 0.19, P < 0.001). On the basis of DMI fungicide sensitivity testing and CYP51 gene sequence polymorphism, isolates from the Distrito Federal separated into two main groups, one with generally higher EC 50 values against eight DMI fungicides. A clear phenotype-to-genotype relationship was observed for isolates carrying the CYP51 alteration Y461N. Conventionally adopted fungicides for control of Sigatoka leaf spot are likely to be overcome by combined sexual and asexual reproduction mechanisms in P. musae driving genetic variability. Continued analysis of pathogen genetic diversity and monitoring of DMI sensitivity profiles of Brazilian field populations is essential for the development of integrated control strategies based on host resistance breeding and rational design of fungicide regimes.

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Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil

Cited by 8 publications

References 35 publications

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

Distribution of Pseudocercospora species causing Sigatoka leaf diseases of banana in Uganda and Tanzania

Genetic Diversity and Azole Fungicide Sensitivity in Pseudocercospora musae Field Populations in Brazil

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