Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

Silva, G. F.; Paixão, Rodrigo; Queiroz, Casley Borges de; Santana, Mateus Ferreira; Souza, A. S.; Sousa, N. R.; Hanada, Rogério Eiji; Gasparotto, L.

doi:10.4238/2014.september.26.7

Cited by 8 publications

(7 citation statements)

References 28 publications

(36 reference statements)

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“…The high gene diversity in the population of M. musicola found in this study ( H E = 0.71) was similar to the values estimated for M. fijiensis ( H E = 0.65) in Southeast Asia, its putative centre of origin (Robert et al ., ), and higher than the values estimated for M. fijiensis in Brazil ( H E = 0.2) (Silva et al ., ). These comparisons are not completely meaningful because different fungal species are being analysed and in one case (Silva et al ., ) a different marker (bi‐allelic) was used. Nevertheless, it can be postulated that the high gene diversity present in the Minas Gerais population is probably due to the presence of the pathogen for a longer time in the region.…”

Section: Discussionmentioning

confidence: 97%

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Gomes

Douhan²,

Lehner

et al. 2017

Plant Pathology

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The genetic structure of Mycosphaerella musicola has never been investigated in Brazil to address epidemiologically related questions associated with yellow sigatoka. A total of 223 single‐conidium isolates from four regions (North, South, Zona da Mata and Triângulo Mineiro) of Minas Gerais State, Brazil, were used to assess the population genetic structure of M. musicola. Isolates were characterized regarding the frequency of the MAT1‐1‐1 or MAT1‐2‐1 idiomorphs and polymorphism at nine microsatellite loci. The mating‐type ratio in three of the four subpopulations was c. 1:1. A total of 87 alleles and 216 multilocus genotypes were identified. The overall population was in linkage equilibrium. Most (93.9%) genetic variation was detected within the subpopulations and there was weak differentiation between them. In total, eight genetic groups were detected and isolates of seven groups were present in all regions. The population of M. musicola in Minas Gerais seems to have high evolutionary potential: it is panmictic and both sexual reproduction and gene flow affect genetic variability. Strategies to avoid fungicide resistance should be enforced and breeding programmes need to consider quantitative resistance in the banana cultivars.

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Section: Discussionmentioning

confidence: 97%

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Gomes

Douhan²,

Lehner

et al. 2017

Plant Pathology

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show abstract

“…In this study, two Salmonella-positive overshoe samples collected from one person on farm A yielded an identical gel pattern, further demonstrating the reliability and accuracy of the ERIC-PCR. The technique has been successfully applied to the typing of numerous bacterial strains (Dorneles et al, 2014;Silva et al, 2014) and to diversity analyses of bacterial communities, including Salmonella (Pang et al, 2007;Cao et al, 2008). In the present study, ERIC-PCR was used for tracing down the source of the contaminations.…”

Section: Eric-pcr Fingerprinting and Clustering Analysismentioning

confidence: 99%

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Liu

et al. 2018

J. Poult. Sci.

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Salmonella is the most common cause of foodborne illnesses worldwide. Poultry eggs are a major contamination source of Salmonella. The prevalence of Salmonella has been effectively reduced since a series of measures were taken to reduce contamination in egg-laying houses. In the present study, 1,512 environmental samples obtained from layer farms of different production scales were screened in a voluntary Salmonella survey study. Contaminations were detected using a PCR method. Genetic relationships among Salmonella samples were specified using molecular typing by enterobacterial repetitive intergenic consensus (ERIC)-PCR. The survey results showed that two layer farms, located in the Shandong and Hebei provinces, were contaminated with Salmonella. Thirty-one samples from these two farms, including feed, drinking nipples, egg collection belt, air inlets and outlets, air, overshoes, and eggshells, were identified as Salmonella-positive. It was observed that certain samples within the henhouses as well as in the egg collecting areas showed relatively high genetic similarities. The survey conclusively revealed minor Salmonella contamination in northern China. Moreover, various areas within the layer farms were identified as part of the propagation chain of Salmonella. Furthermore, evidence of cross-contamination of Salmonella was found in the laying houses and egg collection areas, even between these two regions. Therefore, it is necessary to establish routine Salmonella detection and subsequent environmental control measures in order to decrease the prevalence of Salmonella.

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“…In Brazil, BLSD causes significant losses in the humid areas of the Amazon (Gasparotto et al, 2006), affecting bananas of the 'Prata' and 'Cavendish' subgroups (Silva et al, 2014). The disease spreads through the spores of the fungus Mycosphaerella fijiensis (Silva et al, 2014), which could happen during the transport of banana plant material that could contain the pathogen propagules, causing concern in the banana agribusiness. As a result, strategies for the genetic improvement and the development of cultivars resistant to BLSD are being sought in Brazil .…”

Section: Introductionmentioning

confidence: 99%

Performance of banana genotypes with resistance to black leaf streak disease in Northeastern Brazil

Weber

Garruti

Norões³

et al. 2017

Pesq. agropec. bras.

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Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

Cited by 8 publications

References 28 publications

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Performance of banana genotypes with resistance to black leaf streak disease in Northeastern Brazil

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