Distribution of MUC1 in the normal human oral cavity is localized to the ducts of minor salivary glands

Sengupta, Anita; Valdramidou, Dimitra; Huntley, Suzanne; Hicks, Sally J.; Carrington, Stephen D.; Corfield, Anthony P.

doi:10.1016/s0003-9969(01)00010-3

Cited by 42 publications

(31 citation statements)

References 44 publications

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“…Labeling for MG1 was occasionally present along the apical membrane of the duct cells, presumably due to binding of secreted mucin. Membraneassociated and adherent mucins at the luminal surface of the duct cells may function to maintain duct patency by preventing adherence of the luminal membranes of opposing cells (Hilkens et al 1992;Sengupta et al 2001). Liu et al (2002) have suggested that the cytoplasmic domain of membrane-associated mucins may be involved in signal transduction.…”

Section: Resultsmentioning

confidence: 99%

Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

Piludu

Rayment²,

Liu³

et al. 2003

J Histochem Cytochem.

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S U M M A R YThe human salivary mucins MG1 and MG2 are well characterized biochemically and functionally. However, there is disagreement regarding their cellular and glandular sources. The aim of this study was to define the localization and distribution of these two mucins in human salivary glands using a postembedding immunogold labeling method. Normal salivary glands obtained at surgery were fixed in 3% paraformaldehyde-0.1% glutaraldehyde and embedded in Lowicryl K4M or LR Gold resin. Thin sections were labeled with rabbit antibodies to MG1 or to an N-terminal synthetic peptide of MG2, followed by gold-labeled goat anti-rabbit IgG. The granules of all mucous cells of the submandibular and sublingual glands were intensely reactive with anti-MG1. No reaction was detected in serous cells. With anti-MG2, the granules of both mucous and serous cells showed reactivity. The labeling was variable in both cell types, with mucous cells exhibiting a stronger reaction in some glands and serous cells in others. In serous granules, the electron-lucent regions were more reactive than the dense cores. Intercalated duct cells near the acini displayed both MG1 and MG2 reactivity in their apical granules. In addition, the basal and lateral membranes of intercalated duct cells were labeled with anti-MG2. These results confirm those of earlier studies on MG1 localization in mucous cells and suggest that MG2 is produced by both mucous and serous cells. They also indicate differences in protein expression patterns among salivary serous cells.

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Section: Resultsmentioning

confidence: 99%

Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

Piludu

Rayment²,

Liu³

et al. 2003

J Histochem Cytochem.

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“…It has been shown that the major and minor salivary glands, as well as oral epithelial cells, express the membrane-bound mucin MUC1 [13,14]. MUC1 is ubiquitously expressed in the epithelial cells lining the respiratory, reproductive, and gastrointestinal tracts.…”

Section: Introductionmentioning

confidence: 99%

Mucin levels in saliva of adolescents with dental caries

et al. 2014

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BackgroundHuman saliva, a complex secretion that contains a mixture of inorganic and organic molecules, plays an essential role in the maintenance of oral health. Mucins are the major macromolecular component of the secretion and are considered the first line of defense for epithelial tissues. The aim of this study was to compare levels of mucins (MUC5B, MUC7, and MUC1) in saliva of young subjects with dental caries.Material/MethodsAll patients had DMF (decay/missing/filled) higher than value 0. Eight subjects with DMF=3 (control group) and 27 adolescents with DMF >11 (research group) were recruited for this study. Clinical evaluation procedures were oral examination, including tooth, periodontal, oral mucosal status, and collection of saliva samples. Saliva was collected for mucin assay. Enzyme-linked immunosorbent assay was used to quantitate MUC5B, MUC7, and MUC1.ResultsOur results indicate that adolescents with very high intensity of dental caries disease had increased levels of MUC1 and MUC5B. The membrane mucin MUC1 protein levels in the group with DMF>11 (research group) were higher compared to the group with DMF=3 (control group), and the increase was statistically significant (p=0.011). Similarly, secreted mucin MUC5B protein levels were higher (p=0.06) in the group with DMF>11 (research group). Although MUC7 protein levels were slightly reduced in symptomatic subjects, the decrease was statistically insignificant (p=0.918).ConclusionsOur data suggest links between the production of mucins, especially MUC1 and MUC5B in saliva, and dental caries disease.

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“…The minor salivary glands are suggested to play a part in protecting the oral mucosa, because their secretions are rich in mucins [12]. MUC1 has been shown to be prominent in the mucosal pellicle of the buccal mucosa [13].…”

Section: Discussionmentioning

confidence: 99%

“…Secreted salivary mucins, MUC5B and MUC7, are the most important glycoproteins found in saliva [10]. The membrane-associated mucins (MAMs), MUC1 and MUC4, are produced in all salivary glands [11,12] and they play a role in pellicle formation at the mucosal surfaces [13,14]. MAMs are considered to be multi-functional proteins.…”

mentioning

confidence: 99%

Microstructure of Oral Epithelial Cells as an Underlying Basis for Salivary Mucosal Pellicle

Kullaa

Asikainen²,

Herrala

et al. 2014

Ultrastructural Pathology

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Distribution of MUC1 in the normal human oral cavity is localized to the ducts of minor salivary glands

Cited by 42 publications

References 44 publications

Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

Mucin levels in saliva of adolescents with dental caries

Microstructure of Oral Epithelial Cells as an Underlying Basis for Salivary Mucosal Pellicle

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