Distribution of NADPH-diaphorase positive nerve fibers in the rat nasal mucosa

Hanazawa, Toyoyuki; Motosugi, Hideaki; Konno, Akiyoshi; Kaneko, Toshio; Tanaka, Kōichi; Chiba, Tetsuhiro

doi:10.1016/0304-3940(93)90801-q

Cited by 36 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous researchers noted that the distribution pattern of NO nerves in the nasal epithelium overlapped with that of CGRP-containing nerves, forming a basal plexus within the epithelium extending upwards, close to the luminal surface (FISCHER et al, 1993;HANAZAWA et al, 1993;. Besides these findings, the presence of NADPH-diaphorase and NOS in the trigeminal ganglion suggested that NO-containing intraepithelial nerves might be sensory in nature.…”

Section: Discussionmentioning

confidence: 70%

“…Several previous studies using the enzyme histochemistry for NADPH-diaphorase and immunohistochemistry for NOS have described the presence of NO nerve fibers in the nasal and tracheal respiratory epithelia which are believed to be innervated mainly by sensory fibers (FISCHER et al, 1993;HANAZAWA et al, 1993;.…”

Section: Discussionmentioning

confidence: 99%

“…It has further been suggested that NO-containing intraepithelial nerve fibers originate from the trigeminal ganglia (HANAZAWA et al, 1993;. This assumption was based on the knowledge that no neuropeptide other than CGRP and SP was found in the intraepithelial nerves of the 450 S. H. LEE, T. IwANACA and T. FUJITA: nasal mucosa as described above.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Intraepithelial Nerve Fibers in the Nasal Mucosa of the Rat with Special Reference to the Localization of CGRP, VIP and Nitric Oxide (NO).

Lee

Iwanaga

Fujita

1995

Archives of Histology and Cytology

View full text Add to dashboard Cite

Summary. Previous studies have demonstrated that the epithelium of the respiratory portion of rat nasal mucosa is amply supplied by nerve fibers with immunoreactivities for calcitonin gene-related peptide (CGRP) and substance P (SP), these fibers most likely acting as sensory mediators in the mucosa. The present study demonstrates that some intraepithelial fibers contain a VIP-immunoreactivity whose occurrence in these nerves has previously been neglected. The present study further aims to confirm the occurrence of NO-producing intraepithelial nerve fibers in the rat nasal mucosa and to examine its colocalization with CGRP and with VIP.Double staining methods were used to evaluate the colocalization of NADPH-diaphorase reactivity and CGRP-or VIP-immunoreactivity.The reactivity for NADPH-diaphorase and that for CGRP coexisted in only a small part, if any, of the nerve fibers distributed at the basal portion of the epithelium. In the perpendicularly and obliquely oriented transepithelial nerve fibers, both reactivities were clearly demonstrated to be separated in different fibers.VIP immunoreactivity was also present in a part of the intraepithelial nerve fibers of the nasal mucosa, and their entire population was shown to be positive for NADPH-diaphorase.The NADPH-diaphorase-positive reaction was displayed in only a small population of neurons in the trigeminal ganglion, whereas it was seen in numerous neurons in sphenopalatine ganglion, being colocalized with VIP.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Intraepithelial Nerve Fibers in the Nasal Mucosa of the Rat with Special Reference to the Localization of CGRP, VIP and Nitric Oxide (NO).

Lee

Iwanaga

Fujita

1995

Archives of Histology and Cytology

View full text Add to dashboard Cite

show abstract

“…Anatomical analogy between the penile corpus cavernosum and the nasal tissue suggests that NO may be involved in the vasomotor regulation of both of these vessels. Indeed, NO synthase is abundant in the parasympathetic neural tissues in the pterygopalatine ganglion and the nasal mucosa [17,18]. It is noteworthy that NO synthase is also abundant in the pelvic plexus, corpus cavernosum and membranous urethra [19].…”

Section: Exercise and Nasal Airway Nomentioning

confidence: 99%

Measurement of nitric oxide in human nasal airway

Imada

Iwamoto²,

Nonaka³

et al. 1996

Eur Respir J

View full text Add to dashboard Cite

The nasal output of nitric oxide (NO) is known to be high, but there have been varying reports of the exact level. We attempted to establish a quantitative measurement of nasal NO, and looked for a possible relationship with nasal resistance, at rest and during exercise.Nasal airway ventilation was performed by using an air pump at a constant flow rate, whilst the soft palate was elevated voluntarily. In a preliminary study, the flow rate for sampling was changed and concentrations of NO were measured. After determination of flow rate, rhinomanometry for nasal resistance and measurement of nasal NO by chemiluminescence were carried out before and after moderate exercise.The These data indicate that nasal NO can be measured quantitatively as V 'NO and might be involved in the control of nasal resistance. Eur Respir J., 1996, 9, 556- [6]. However, quantitative measurement has been performed in only a few studies on exhaled NO [7,8] and nasal NO [9]. In the nasal airway, NO was first detected by ALVING et al. [2] (1993). These authors found that most exhaled NO comes from the nose because of some 10 fold higher concentration of nasal NO than of exhaled NO. In general, a concentration of a gas rising from the wall of the lumen should vary with the amount of ventilation. Accordingly, the concentration of exhaled NO has been reported to correlate hyperbolically with a ventilatory volume, whereas the net output of NO (V'NO) remained constant [8]. We applied the same method in attempting to establish a quantitative measurement of the nasal airway NO, and investigated the relationship between nasal airway NO and the nasal resistance before and after exercise. Materials and methods Subjects and protocolTwelve male subjects (aged 27-35 years), with no allergies or abnormal rhinoscopic findings, volunteered for this study. The protocol of the study was reviewed and approved by the Asahikawa Medical College Institutional Review Board for Research. Subjects rested in a sitting position for at least 15 min, during the last 5 min of which rhinomanometry and the measurement of nasal airway NO were performed. Subjects then walked on a 10°graded treadmill at 5 km·h -1 for 4 min. Immediately after exercise, subjects sat down and the measurements were repeated. RhinomanometryThe anterior method of rhinomanometry [10] was applied to obtain nasal resistance of each side of the nasal airway. From the flow-pressure curve obtained by rhinomanometry (model SR-11, Rion, Japan), nasal resistance at 100 Pa was taken as the nasal resistance value (Pa·s·cm -3 ) for each subject. The total nasal resistance for both nasal airways was obtained by calculating the sum of the reciprocals of parallel resistance for each nasal airway. Measurement of nasal airway NOThe quantitative measurement of NO in the human exhaled gas has already been established [8]:

show abstract

“…Furthermore, biochemical abnormalities of the serotonergic system associated with PD may be a direct result of serotonin transporter abnormalities. Neurochemical studies have indicated a decrease in striatal and cortical serotonin and a reduction in cerebrospinal fluid serotonin concentration in PD patients [8, 9]. …”

Section: Introductionmentioning

confidence: 99%

The Serotonin Transporter Gene and Parkinson’s Disease

McCann

McManus²,

Johnson³

et al. 2000

Eur Neurol

View full text Add to dashboard Cite

Dysfunction in the serotonin (5-hydroxytryptamine) system and reduced serotonin concentrations have been reported in patients with Parkinson’s disease (PD). Serotonin concentrations in neural tissue are controlled by a presynaptic serotonin transporter protein that is encoded by a single gene. Therefore, we investigated whether a polymorphic region in the serotonin transporter gene is associated with PD. Three variable-number tandem repeat (VNTR) elements of the serotonin transporter gene were detected by polymerase chain reaction, those with 9, 10, 11 and 12 copies of the repeat element. The 10-copy VNTR element was significantly less common in patients with PD than controls in the univariate analysis (p < 0.05). Logistic regression analysis revealed no significant differences between patients (n = 198) and controls (n = 200) in the distribution frequencies of 9- and 12-copy alleles and combined genotypes (odds ratio = 1.20; p = 1.71). A positive family history of PD was a strong predictor of disease risk (odds ratio = 2.98; 95% confidence interval 1.51–5.87; p = 0.001). Although slight differences were observed between patient and control groups, these data suggest that defects in serotonin concentrations in patients with PD are unlikely to be due to polymorphisms in the serotonin transporter gene in this large Australian cohort; however, the inverse association observed with the 10-copy allele warrants further investigation.

show abstract

Distribution of NADPH-diaphorase positive nerve fibers in the rat nasal mucosa

Cited by 36 publications

References 19 publications

Intraepithelial Nerve Fibers in the Nasal Mucosa of the Rat with Special Reference to the Localization of CGRP, VIP and Nitric Oxide (NO).

Intraepithelial Nerve Fibers in the Nasal Mucosa of the Rat with Special Reference to the Localization of CGRP, VIP and Nitric Oxide (NO).

Measurement of nitric oxide in human nasal airway

The Serotonin Transporter Gene and Parkinson’s Disease

Contact Info

Product

Resources

About