Distribution of Oncogenic Human Papillomavirus Genotypes at High Grade Cervical Lesions above CIN 2 Grade with Histological Diagnosis

Kim, Geehyuk; Park, Sungyoung; Wang, Hye-Young; Kim, Sung-Hyun; Park, Sangjung; Yu, Kwangmin; Lee, Boohyung; Ahn, Seung-Ju; Kim, Eun-Joong; Lee, Dongsup

doi:10.15616/bsl.2016.22.2.37

Cited by 2 publications

(4 citation statements)

References 23 publications

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“…A similar observation was reported in studies from two African countries-Malawi and Ghana having multiple HPV infections that accounted for 54.0% and 52.2% respectively [19, 25]. Lower rates of multiple HPV infection were reported in studies from Croatia [11], Serbia [15], India [17], Thailand [18], Ibadan Nigeria [21], Pakistan [35], Republic of Benin [41], USA [42], Brazil [43], Korea [44] and Sri Lanka [45]. Although most of the studies from Africa have observed multiple HPV infections, the finding in this study was as expected.…”

Section: Discussionsupporting

confidence: 75%

“…However, most studies have identified that multiple HPV types exhibit synergism and additivity and were associated with persistent infection, higher risk of disease progression, disease severity and poor survival [17, 43, 47, 48]. Conversely, Salazar et al [42] and Wang et al [50] have shown no significant difference between single and multiple HPV infection, but a reduced rate of high-grade cervical lesions. Intergenotypic competition or effective immune response has been suggested as a possible mechanism for a reduced rate of high-grade cervical lesions in multiple HPV infections [42].…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, Salazar et al [42] and Wang et al [50] have shown no significant difference between single and multiple HPV infection, but a reduced rate of high-grade cervical lesions. Intergenotypic competition or effective immune response has been suggested as a possible mechanism for a reduced rate of high-grade cervical lesions in multiple HPV infections [42].…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Kabir¹,

Bukar²,

Nggada³

et al. 2019

Pan Afr Med J

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IntroductionCervical cancer is the commonest gynaecological malignancy and the second most common cancer among women worldwide. Several epidemiological, clinical and molecular studies have strongly implicated oncogenic high-risk human papillomavirus infection in the aetiopathogenesis of cervical cancer. The objectives of this study were to determine the cervical HPV prevalence and genotype distribution in cervical cancer in Maiduguri, Nigeria.MethodsThis was a descriptive and retrospective study. Sixty-three archived paraffin-embedded tissue blocks with confirmed diagnoses of cervical cancer during the study period (2013-2015) were retrieved and examined. The procedure included deparaffinization of tissue samples, DNA extraction, PCR, gel electrophoresis, and HPV genotyping by reverse hybridization line probe assay.ResultsSixty-three cervical cancer cases were subjected to genomic DNA extraction and HPV-DNA detection by PCR. Fifty-eight samples showed PCR positivity while 5 samples were PCR negative. HPV-specific DNA was detected in 44 of the 58 PCR-positive samples and thus the prevalence was 69.8%. Ten different high-risk HPV genotypes were detected. Both single and multiple high-risk HPV infections were observed. The most prevalent type of the human papillomavirus detected was HPV16.ConclusionHPV-DNA was prevalent in majority of the examined cervical cancer tissues and that HPV16, HPV18, HPV45, HPV51 and HPV52 were the predominant HPVs detected in both single and multiple HPV infections. The results of this study and further studies will provide more detailed information about HPV and may contribute significantly to the prevention of cervical cancer through primary high-risk HPV testing and HPV vaccination against the oncogenic viruses.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Kabir¹,

Bukar²,

Nggada³

et al. 2019

Pan Afr Med J

View full text Add to dashboard Cite

show abstract

“…HPV screening test is done by molecular diagnostic technology such as PCR, real-time PCR, and NASBA method using HPV DNA or RNA [17][18][19][20]. Especially, real-time PCR is more sensitive and specific molecular diagnostic method to detect live HPV in patients compared to other methods [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Commercial Complementary DNA Synthesis Kits for Detecting Human Papillomavirus

Park

Chang

et al. 2019

Korean J Clin Lab Sci.

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Cervical cancer is the fourth most common malignant neoplasm in women worldwide. Most cases of cervical cancer are caused by an infection by the human papillomavirus. Molecular diagnostic methods have emerged to detect the HPV for sensitivity, specificity, and objectivity. In particular, real-time PCR has been introduced to acquire a more sensitive target DNA or RNA. RNA extraction and complementary DNA synthesis are proceeded before performing real-time PCR targeting RNA. To identify an adequate and sensitive cDNA synthesis kit, this study evaluated the two commonly used kits for cDNA synthesis. The results show that the R 2 and efficiency (%) of the two cDNA synthesis kits were similar in the cervical cancer cell lines. On the other hand, the Takara kit compared to Invitrogen kit showed P＜0.001 in the 10 2 and 10 3 SiHa cell count. The Takara kit compared to the Invitrogen kit showed P＜0.001 in the 10 1 and 10 2 HeLa cell count. Furthermore, 8, 4, 2, 1, and 0.5 ml of forty exfoliated cell samples were used to compare the cDNA synthesis kits. The Takara kit compared to the Invitrogen kit showed P＜0.01 in 8, 4, and 1 ml and P＜0.05 in 0.5 mL. The study was performed to identify the most appropriate cDNA synthesis kit and suggests that a cDNA synthesis kit could affect the real-time PCR results.

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Distribution of Oncogenic Human Papillomavirus Genotypes at High Grade Cervical Lesions above CIN 2 Grade with Histological Diagnosis

Cited by 2 publications

References 23 publications

Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Evaluation of Commercial Complementary DNA Synthesis Kits for Detecting Human Papillomavirus

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