Distribution of PAHs and the PAH-degrading bacteria in the deep-sea sediments of the high-latitude Arctic Ocean

Dong, Chunming; Bai, X.; Sheng, Haoyue; Jiao, Liping; Hong, Zhou; Shao, Zongze

doi:10.5194/bg-12-2163-2015

Cited by 87 publications

(44 citation statements)

References 68 publications

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“…Likewise, culture-independent studies supported that Cycloclasticus spp. likely play a major role in the degradation of petroleum polycyclic aromatic hydrocarbons in coastal (Coulon et al, 2012), deep-sea (Cui et al, 2008; Dong et al, 2015; Gutierrez et al, 2015), and estuarine (McKew et al, 2007; Niepceron et al, 2010) sediments.…”

Section: Resultsmentioning

confidence: 99%

Microbial Community Response to Simulated Petroleum Seepage in Caspian Sea Sediments

et al. 2017

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Anaerobic microbial hydrocarbon degradation is a major biogeochemical process at marine seeps. Here we studied the response of the microbial community to petroleum seepage simulated for 190 days in a sediment core from the Caspian Sea using a sediment-oil-flow-through (SOFT) system. Untreated (without simulated petroleum seepage) and SOFT sediment microbial communities shared 43% bacterial genus-level 16S rRNA-based operational taxonomic units (OTU0.945) but shared only 23% archaeal OTU0.945. The community differed significantly between sediment layers. The detection of fourfold higher deltaproteobacterial cell numbers in SOFT than in untreated sediment at depths characterized by highest sulfate reduction rates and strongest decrease of gaseous and mid-chain alkane concentrations indicated a specific response of hydrocarbon-degrading Deltaproteobacteria. Based on an increase in specific CARD-FISH cell numbers, we suggest the following groups of sulfate-reducing bacteria to be likely responsible for the observed decrease in aliphatic and aromatic hydrocarbon concentration in SOFT sediments: clade SCA1 for propane and butane degradation, clade LCA2 for mid- to long-chain alkane degradation, clade Cyhx for cycloalkanes, pentane and hexane degradation, and relatives of Desulfobacula for toluene degradation. Highest numbers of archaea of the genus Methanosarcina were found in the methanogenic zone of the SOFT core where we detected preferential degradation of long-chain hydrocarbons. Sequencing of masD, a marker gene for alkane degradation encoding (1-methylalkyl)succinate synthase, revealed a low diversity in SOFT sediment with two abundant species-level MasD OTU0.96.

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Section: Resultsmentioning

confidence: 99%

Microbial Community Response to Simulated Petroleum Seepage in Caspian Sea Sediments

et al. 2017

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“…Although, the concentrations of PAHs and polysaccharides of the samples were not determined in this study due to the difficulty of sampling and the limited amount of samples we obtained, these organics have been reported to be common in the deep ocean (Simoneit and Fetzer, 1996; Dong et al, 2015). More than half of the actinobacterial isolates obtained from this research showed the capacity to degrade organic matter, suggesting that actinobacteria may contribute to the substance and energy flows in biogeochemical cycles in the deep ocean.…”

Section: Discussionmentioning

confidence: 96%

“…PAHs are the main components of petroleum and are introduced into the marine environment by oil spill, river import, natural seepage, hydrothermal activity, and even air current transfer (Latimer and Zheng, 2003). PAHs are persistent organic pollutants that tend to accumulate in marine sediments and have even been reported to be widespread in the deep Arctic Ocean and Pacific Ocean (Simoneit and Fetzer, 1996; Dong et al, 2015). A recent study indicated that hydrocarbon-degrading actinobacteria, notably Dietzia , were abundant in the Arctic deep-sea sediments (0–0.5 mbsf) and were likely to play an important role in PAH degradation in situ (Dong et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Diversity, Biogeography, and Biodegradation Potential of Actinobacteria in the Deep-Sea Sediments along the Southwest Indian Ridge

et al. 2016

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The phylum Actinobacteria has been reported to be common or even abundant in deep marine sediments, however, knowledge about the diversity, distribution, and function of actinobacteria is limited. In this study, actinobacterial diversity in the deep sea along the Southwest Indian Ridge (SWIR) was investigated using both 16S rRNA gene pyrosequencing and culture-based methods. The samples were collected at depths of 1662–4000 m below water surface. Actinobacterial sequences represented 1.2–9.1% of all microbial 16S rRNA gene amplicon sequences in each sample. A total of 5 actinobacterial classes, 17 orders, 28 families, and 52 genera were detected by pyrosequencing, dominated by the classes Acidimicrobiia and Actinobacteria. Differences in actinobacterial community compositions were found among the samples. The community structure showed significant correlations to geochemical factors, notably pH, calcium, total organic carbon, total phosphorus, and total nitrogen, rather than to spatial distance at the scale of the investigation. In addition, 176 strains of the Actinobacteria class, belonging to 9 known orders, 18 families, and 29 genera, were isolated. Among these cultivated taxa, 8 orders, 13 families, and 15 genera were also recovered by pyrosequencing. At a 97% 16S rRNA gene sequence similarity, the pyrosequencing data encompassed 77.3% of the isolates but the isolates represented only 10.3% of the actinobacterial reads. Phylogenetic analysis of all the representative actinobacterial sequences and isolates indicated that at least four new orders within the phylum Actinobacteria were detected by pyrosequencing. More than half of the isolates spanning 23 genera and all samples demonstrated activity in the degradation of refractory organics, including polycyclic aromatic hydrocarbons and polysaccharides, suggesting their potential ecological functions and biotechnological applications for carbon recycling.

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“…Petroleum components have traditionally been divided into four fractions: saturated hydrocarbons, aromatics, resins, and asphaltenes [20]. Normally, saturated hydrocarbons, such as short-chain saturated hydrocarbons and low-molecular-weight aromatics, are the most susceptible to biodegradation, whereas PAHs in aromatics and resins are less vulnerable to microbial attack [21].…”

Section: Introductionmentioning

confidence: 99%

Removal Capacities of Polycyclic Aromatic Hydrocarbons (PAHs) by a Newly Isolated Strain from Oilfield Produced Water

Wang

et al. 2017

IJERPH

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The polycyclic aromatic hydrocarbon (PAH)-degrading strain Q8 was isolated from oilfield produced water. According to the analysis of a biochemical test, 16S rRNA gene, house-keeping genes and DNA–DNA hybridization, strain Q8 was assigned to a novel species of the genus Gordonia. The strain could not only grow in mineral salt medium (MM) and utilize naphthalene and pyrene as its sole carbon source, but also degraded mixed naphthalene, phenanthrene, anthracene and pyrene. The degradation ratio of these four PAHs reached 100%, 95.4%, 73.8% and 53.4% respectively after being degraded by Q8 for seven days. A comparative experiment found that the PAHs degradation efficiency of Q8 is higher than that of Gordonia alkaliphila and Gordonia paraffinivorans, which have the capacities to remove PAHs. Fourier transform infrared spectra, saturate, aromatic, resin and asphaltene (SARA) and gas chromatography–mass spectrometry (GC–MS) analysis of crude oil degraded by Q8 were also studied. The results showed that Q8 could utilize n-alkanes and PAHs in crude oil. The relative proportions of the naphthalene series, phenanthrene series, thiophene series, fluorene series, chrysene series, C21-triaromatic steroid, pyrene, and benz(a)pyrene were reduced after being degraded by Q8. Gordonia sp. nov. Q8 had the capacity to remediate water and soil environments contaminated by PAHs or crude oil, and provided a feasible way for the bioremediation of PAHs and oil pollution.

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Distribution of PAHs and the PAH-degrading bacteria in the deep-sea sediments of the high-latitude Arctic Ocean

Cited by 87 publications

References 68 publications

Microbial Community Response to Simulated Petroleum Seepage in Caspian Sea Sediments

Microbial Community Response to Simulated Petroleum Seepage in Caspian Sea Sediments

Diversity, Biogeography, and Biodegradation Potential of Actinobacteria in the Deep-Sea Sediments along the Southwest Indian Ridge

Removal Capacities of Polycyclic Aromatic Hydrocarbons (PAHs) by a Newly Isolated Strain from Oilfield Produced Water

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