Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables

Mizuno, Masayuki; Yurimoto, Hiroya; Yoshida, Naoko; Iguchi, Hiroyuki; Sakai, Yasuyoshi

doi:10.1271/bbb.110737

Cited by 24 publications

(22 citation statements)

References 12 publications

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“…The dominance of Methylobacterium in phyllosphere communities (Holland et al, 2002;Delmotte et al, 2009;Wellner et al, 2011;Mizuno et al, 2012) is coincident with the finding that Methylobacterium-affiliated genotypes were predominant in the mxaF data sets of investigated soils. Methylobacterium-affiliated mxaF genoytpes were statistically indicative for grassland vegetation.…”

Section: Diversity Of Soil Methylotrophssupporting

confidence: 52%

“…As methanol is plant-derived in terrestrial ecosystems, plant surfaces, such as leaves, can be considered as favored habitats of aerobic methylotrophs. It has indeed been demonstrated that leaf surfaces of various temperate plant species are inhabited by active methylotrophs, which constitute about 14-20% to the total microbial community of the phyllosphere (Holland et al, 2002;Delmotte et al, 2009;Fedorov et al, 2011;Wellner et al, 2011;Mizuno et al, 2012). However, the upper soil layers of forests and grasslands are also well supplied with oxygen, and belowground parts of plants are likely sources of methanol.…”

Section: Introductionmentioning

confidence: 99%

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Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

et al. 2012

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The role of soil methylotrophs in methanol exchange with the atmosphere has been widely overlooked. Methanol can be derived from plant polymers and be consumed by soil microbial communities. In the current study, methanol-utilizing methylotrophs of 14 aerated soils were examined to resolve their comparative diversities and capacities to utilize ambient concentrations of methanol. Abundances of cultivable methylotrophs ranged from 10 6 -10 8 g soilDW. Methanol dissimilation was measured based on conversion of supplemented 14 C-methanol, and occurred at concentrations down to 0.002 lmol methanol g soilDW À 1. Tested soils exhibited specific affinities to methanol (a 0 s ¼ 0.01 d À 1 ) that were similar to those of other environments suggesting that methylotrophs with similar affinities were present. Two deep-branching alphaproteobacterial genotypes of mch responded to the addition of ambient concentrations of methanol (p0.6 lmol methanol g soilDW) in one of these soils. Methylotroph community structures were assessed by amplicon pyrosequencing of genes of mono carbon metabolism (mxaF, mch and fae). Alphaproteobacteria-affiliated genotypes were predominant in all investigated soils, and the occurrence of novel genotypes indicated a hitherto unveiled diversity of methylotrophs. Correlations between vegetation type, soil pH and methylotroph community structure suggested that plant-methylotroph interactions were determinative for soil methylotrophs.

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Section: Diversity Of Soil Methylotrophssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

et al. 2012

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“…These techniques have been employed primarily for the detection, isolation, and culture of species of Methylobacterium (PPFMs), which are found in abundance on plant leaf and stem surfaces, and on which there is an extensive literature [49,[72][73][74]. Numerous species and strains of Methylobacterium have been isolated from plants [49,75].…”

Section: Isolation Of Methylotrophs From Plants Leaves and Stems (Thementioning

confidence: 99%

Cultivation of Methylotrophs

Kelly

Ardley

Wood

2014

Springer Protocols Handbooks

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Methylotrophy is the ability of numerous Alpha-, Beta-, and Gammaproteobacteria, Gram-positive bacteria, Actinomycetes, and yeasts to obtain all the energy and carbon required for growth from the metabolism of methyl compounds which have no carbon-carbon bonds. Numerous media and techniques for the isolation and culture of methylotrophs have been described, and a comprehensive description of these protocols is given, with substrates such as methanol, methylamines, and methylated sulfur compounds, and the description of some more complex media. The metabolic and habitat diversity of methylotrophs is outlined, ranging from soil and freshwater organisms, through thermophiles and yeasts, to animal hosts and plant colonisers, including specialised types that induce (and reside in) root nodules in some plant species. The methods for the commercial exploitation of some methylotrophs are outlined. This survey excludes the methanotrophs, growing primarily on gaseous methane, as the cultivation of these is considered elsewhere.

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“…Microbes face many environmental challenges during growth and survival in nature, and they must control growth to adapt to the surrounding conditions. Methanolutilizing bacteria in the genus Methylobacterium are often dominant epiphytes in the microbial community in the phyllospheres of various plants such as rice, soybean, clover, Japanese basil and Arabidopsis thaliana (Mizuno et al, 2012;Vorholt, 2012). Methanolmetabolizing enzymes and the general stress resistance regulator PhyR are required for phyllosphere colonization by Methylobacterium spp.…”

Section: Introductionmentioning

confidence: 99%

KaiC family proteins integratively control temperature‐dependent UV resistance in Methylobacterium extorquens AM1

Iguchi

Yoshida

Fujisawa

et al. 2018

Environ Microbiol Rep

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KaiC protein is the pivotal component of the circadian clock in cyanobacteria. While KaiC family proteins are well-conserved throughout divergent phylogenetic lineages, studies of the physiological roles of KaiC proteins from other microorganisms have been limited. We examined the role of the KaiC proteins, KaiC1 and KaiC2, in the methanol-utilizing bacterium Methylobacterium extorquens AM1. Wild-type M. extorquens AM1 cells exhibited temperature-dependent UV resistance (TDR) under permissive growth temperatures (24 °C -32 °C). Both the phosphorylation of KaiC2 and the intracellular levels of KaiC1 were temperature-dependent, and the TDR phenotype was positively regulated by KaiC1 and negatively regulated by KaiC2. Taken together with biochemical and functional analogies to the KaiC protein of cyanobacteria, our present results suggest that KaiC family proteins function to integrate environmental cues, that is, temperature and UV light, and output appropriate cellular responses to allow cells to adapt to changing environmental conditions.© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

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Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables

Cited by 24 publications

References 12 publications

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

Cultivation of Methylotrophs

KaiC family proteins integratively control temperature‐dependent UV resistance in Methylobacterium extorquens AM1

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