Distribution of sexually transmitted diseases in a group of symptomatic male patients using urine samples and PCR technique / Distribuția bolilor cu transmitere sexuală într-un grup de barbați simptomatici utilizand probe de urina si tehnica PCR

Vică, Mihaela Laura; Junie, Lia Monica; Grad, Alecsandra Iulia; Tătaru, Alexandru; Matei, Horea

doi:10.1515/rrlm-2015-0029

Cited by 1 publication

(1 citation statement)

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“…were recognized as pathogens in numerous clinical presentations. The large number of patients positive for U. urealyticum (34% of the positive patients) identified in our study is in accordance with literature data and the conclusions of our previous studies (7,22,23) indicating that infection with this bacterium remains one of the major causes of STIs in Romania.…”

Section: Discussionsupporting

confidence: 92%

Molecular diagnostic of Ureaplasma urealyticum presence and tetracycline resistance in urine samples

Vică

Matei

Katona

et al. 2021

Revista Romana De Medicina De Laborator

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Sexually transmitted infections (STIs) are among the most common infections in Romania. Infection with Ureaplasma urelyticum is one of the major causes of STIs and can cause serious complications. Although tetracycline is the drug commonly used to treat infections caused by U. urealyticum, several studies indicate the emergence and rapid development of strains resistant to these antibiotics in the United States or Europe. Tetracycline resistance in bacteria is encoded by a number of different genetic determinants but in mycoplasmas the only tetracycline resistance determinant that has been reported is the tetM gene. Tetracycline resistance among Ureaplasma spp. is associated with the presence of the horizontally acquired tetM resistance gene. Our study on bacterial DNA aimed to determine the presence of tetracycline-resistant U. urealyticum strains, by identifying the presence of the tetM gene. We used first void urine samples from 622 STI-suspected subjects. DNA was extracted, purified and amplified via PCR for the simultaneous detection of 6 STIs. 68 patients were diagnosed with U. urealyticum. DNA obtained from these samples was amplified using the tetM gene and U. urealyticum - specific urease gene primers. The urease gene was amplified in all samples, confirming the presence of U. urealyticum. The tetM gene was amplified in 2 samples considered tetracycline-resistant strains. The study confirmed the presence of U. urealyticum strains resistant to tetracycline in Romania. The employed technique can produce quick results both for U. urealyticum detection and determination of its resistance to tetracycline using a single easy-to-collect biological sample.

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Section: Discussionsupporting

confidence: 92%