Distribution of spermine in bacilli and lactic acid bacteria

Abstract

Obligate moderately thermophilic bacilli and obligate moderately thermoacidophilic bacilli contained spermine as the major polyamine in addition to putrescine and spermidine. The identity of spermine was confirmed by thin-layer chromatography and high-performance liquid chromatography before and after treatment with putrescine oxidase. Using these methods, thermospermine and spermine can be separated; thermospermine was not present in these organisms. On the other hand, various facultative thermophiles and mesophilic strains of the genus Bacillus, including alkalophiles and halophiles, lack spermine and other tetraamines. No spermine was detected in several strains of mesophilic or facultative slightly thermophilic lactic acid bacteria, Lactobacillus and Streptococcus.

Bacillus cucumis sp. nov. isolated from the rhizosphere of cucumber (Cucumis sativus)

“…Extraction and analysis of polyamines were carried out according to Altenburger et al (1997), using an HPLC apparatus described by Stolz et al (2007) ] were detected. This polyamine pattern is similar to those of B. subtilis, Bacillus cereus and Bacillus megaterium (Hamana et al, 1989).…”

Biogenic Amines in Dairy Products: Origin, Incidence, and Control Means

“…rhamnosus , Streptococcus thermophilus , and Ent. faecalis (Hamana and others ; Chipeva and others ; Hanfrey and others ). Yet, the wide distribution of such alternative pathways in the gut microbiota (Hanfrey and others ), including members of the Proteobacteria and Firmicutes phyla, which encompass many of the Gram‐negative and Gram‐positive dairy contaminants, does not exclude the production of spermidine or unusual polyamines in dairy products via alternative pathways.…”

Polyamine synthetic ability of the eubacteria Halococcus acetoinfaciens, Halococcus agglomeratus, and Halococcus nondenitrificans

“…Furthermore, their polyamine biosynthetic ability closely resembles that of each other except for arginine decarboxylase activity. Differences in polyamine distribution patterns have been used to assign microorganisms to their proper places in the phylogenetic order, since characteristic differences have been found in certain eubacterial groups (Busse and Auling 1988;Hamana and Matsuzaki 1987, 1990a, 1990bHamana et al 1988aHamana et al , 1989aHamana et al , 1990 Table 1. 4 % NaCl NOTE: Decarboxylated products were detected at a concentration of about 50 (++), 10 (+), 2 (k), or below 1 nmol ( -) per 500 p L from 500 nmo1/500 p L (1 mM) of amino acids.…”