Distribution of Staphylococcus species in dairy cows, workers and shared farm environments

Roberts, Marilyn C.; Garland-Lewis, Gemina; Trufan, Sally J.; Meschke, Scott; Fowler, Heather; Shean, Ryan C.; Greninger, Alexander L.; Rabinowitz, Peter

doi:10.1093/femsle/fny146

Cited by 20 publications

(15 citation statements)

References 27 publications

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“…The bacteriological identification results of different Staphylococcus species isolated from different sources are similar to those Roberts et al [ 27 ] who identified 19 different Staphylococcus spp. isolates from animal and human samples, among which S. haemolyticus S. xylosus, S. saprophyticus , S. sciuri , S. succinus , S. chromogenes and S. aureus were the most prevalent.…”

Section: Discussionsupporting

confidence: 85%

Carvacrol Essential Oil: A Natural Antibiotic against Zoonotic Multidrug-Resistant Staphylococcus Species Isolated from Diseased Livestock and Humans

et al. 2021

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Staphylococcus species cause diseases in animals and humans. The prevalence and antimicrobial profiles of Staphylococcus spp. in animals and human samples in the Minya Governorate, Egypt, were determined, and resistance- and virulence-associated genes were observed in multidrug-resistant (MDR) isolates. Moreover, the antibacterial effect of carvacrol essential oil (EO) on the MDR isolates was studied. A total of 216 samples were aseptically collected from subclinically mastitic cow’s milk (n = 100), sheep abscesses (n = 25) and humans (n = 91). Out of 216 samples, a total of 154 single Staphylococcus species (71.3%) were isolated. The most frequent bacterial isolates were S. aureus (43%), followed by S. schleiferi (25%), S. intermedius (12%), S. xylosus (12%), S. haemolyticus (4.5%), S. epidermidis (2%) and S. aurecularis (1%). Haemolytic activity and biofilm production were detected in 77 and 47% of isolates, respectively. Antimicrobial susceptibility testing showed a high degree of resistance to the most commonly used antimicrobials in human and veterinary practices. The mecA, vanA, vanC1 and ermC resistance genes were detected in 93, 42, 83 and 13% of isolates, respectively. Moreover, hla, icaA and icaD virulence genes were detected in 50, 75 and 78% of isolates, respectively. Carvacrol effectively inhibited the growth of all tested isolates at concentrations of 0.1, 0.05 and 0.04% while a concentration of 0.03% inhibited 75% of isolates. Interestingly, some phenotypic changes were observed upon treatment with a carvacrol oil concentration of 0.03%. All the treated MDR Staphylococcus isolates changed from multidrug resistant to either susceptible or intermediately susceptible to 2–3 antimicrobials more than parental bacterial isolates. Real-time PCR was applied for the detection of the differential expression of mecA and vanC1 genes before and after treatment with carvacrol which revealed a mild reduction in both genes’ expression after treatment. Staphylococcus spp. Containing MDR genes are more likely to spread between humans and animals. From these results, carvacrol EO is a promising natural alternative to conventional antimicrobials for pathogens impacting human health and agriculture due to its potential antimicrobial effect on MDR pathogens; even in sub-lethal doses, carvacrol EO can affect their phenotypic properties and genes’ expression.

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Section: Discussionsupporting

confidence: 85%

Carvacrol Essential Oil: A Natural Antibiotic against Zoonotic Multidrug-Resistant Staphylococcus Species Isolated from Diseased Livestock and Humans

et al. 2021

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“…In this study Staphylococcus spp had the highest percentage occurrence of 23.8 % which may be associated with mastitis (Roberts et al, 2018). This was followed by E. coli (21.2 %) and Bacillus spp (12.6 %) while…”

Section: Discussionmentioning

confidence: 49%

Antibiotic Resistance in Bacterial Pathogens Isolated From Cattle Dung and Its Contaminated Soil

Obuekwe¹,

Offodile²

2020

AJHSE

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Cattle dung is used as organic fertilizer and alternative source of fuel or biogas but could also be a source of antibiotic resistance genes in the environment. This study isolated, identified and assessed antibiotic susceptibility pattern of bacteria from cattle dung and its contaminated soil. Bacteria isolation and identification were based on standard techniques while hemolytic activity was used to confirm potential pathogenic bacteria. Antibiotic susceptibility pattern of isolated pathogens were assayed by disk diffusion method. Among isolated bacteria, Staphylococcus spp had highest occurrence of 23.8 % while Micrococcus spp was the least at 1.3 %. Hemolytic bacteria isolates were Staphylococcus aureus (16.5 %), Bacillus spp (17.4 %), Nocardia spp (4.6 %), Escherichia coli (29.4 %), Pseudomonas spp (13.8 %), Serratia marcersens (2.8 %) and Salmonella spp (15.6 %). High resistance (100 %) against Ampiclox (30 µg) was observed in all Staphylococcus aureus and Bacillus spp isolates while Pseudomonas aeruginosa isolates showed 100 % resistance to Ofloxacin (30 µg). Most Gram-positive bacterial isolates were majorly resistant to Beta lactams while Gram negative bacteria were resistant to Fluoroquinolones antibiotics. Multiple antibiotics resistant index (MARI) was measured at greater than 0.2, and was observed in 71.5 % of the hemolytic pathogens. Antibiotics resistance in hemolytic bacterial pathogens from this study is indicative of environmental sources of antibiotic resistance and possible adverse effects on human health.

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“…The next, is Staphylococcus spp. According to Roberts et al [23], Staphylococcus spp were found on human, animal and environment. Olatunji et al [20] equally isolated S. aureus from cow's milk.…”

Section: Discussionmentioning

confidence: 99%

Bacteriological Quality of Raw Cow’s Milk Sold in Minna Central Market, Niger State, Nigeria

Attah

Abalaka

Jesse

et al. 2021

IJPR

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Milk is a non-transparent, yellowish-white substance secreted by the mammary glands of all mammals. It contains proteins, minerals, carbohydrates, fat, and vitamins that meet the dietary requirements of the body than any food in a single diet. As a result of their highly nutritious nature, milk supports the rapid growth of many microorganisms, including bacterial pathogens. The aim of this study was to determine the bacteriological quality of raw cow's milk sold by different vendors in Minna central market, Niger state, Nigeria by pour plate method. The highest bacterial count was 7.5 107 CFU/mL and the lowest bacterial count was 2.5 107 CFU/mL. Bacteria isolated from five different cow's milk samples were identified through their cultural and biochemical properties to be Bacillus badius, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Salmonella typhi. The frequency occurrence of the bacterial isolates were Bacillus spp. (61.9%), Staphylococcus spp. (19.0%), E. coli (14.3%)and Salmonella spp. (5%). The presence of these bacterial isolates and the colony forming unity count observed in these samples indicated poor hygiene and sanitation during milking and post milking processes. Therefore, efforts should be intensified to pasteurize the milk before consumption in order to guarantee the safety of the consumers.

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Distribution of Staphylococcus species in dairy cows, workers and shared farm environments

Cited by 20 publications

References 27 publications

Carvacrol Essential Oil: A Natural Antibiotic against Zoonotic Multidrug-Resistant Staphylococcus Species Isolated from Diseased Livestock and Humans

Carvacrol Essential Oil: A Natural Antibiotic against Zoonotic Multidrug-Resistant Staphylococcus Species Isolated from Diseased Livestock and Humans

Antibiotic Resistance in Bacterial Pathogens Isolated From Cattle Dung and Its Contaminated Soil

Bacteriological Quality of Raw Cow’s Milk Sold in Minna Central Market, Niger State, Nigeria

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