Distribution of the CD68 macrophagr/myeloid associated antigen

Pulford, Karen; Sipos, Attila; Cordell, Jacqueline; Stross, W P; Mason, D Y

doi:10.1093/intimm/2.10.973

Cited by 178 publications

(140 citation statements)

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“…S3). In agreement with historic literature (31,32), these results thus indicate that CD68 is not a macrophage-specific marker in human BC.…”

Section: Resultssupporting

confidence: 92%

“…Although used successfully in multiple studies to relate TAM infiltration with clinically relevant outcomes, our results indicate that CD68 alone cannot accurately evaluate macrophage presence in human breast tissue given that multiple stromal cells express it and that a subset of these are CSF1R-and CD45-negative. We observed that the nonleukocytic CD68 + cells were predominantly located within tumor stroma and, thus, based on this localization and morphology, we speculate that CSF1R − CD68 + cells likely reflect tumor-associated fibroblasts or monocyte-derived fibrocytes in agreement with other reports (31,32,(49)(50)(51)(52). Our findings do not invalidate CD68 as a clinically relevant marker and, importantly, CSF1-response gene signatures have been identified in breast adenocarcinomas that are predictive of recurrence risk and metastasis (53,54).…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Leukocyte composition of human breast cancer

Ruffell¹,

Rugo

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

405

300

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Retrospective clinical studies have used immune-based biomarkers, alone or in combination, to predict survival outcomes for women with breast cancer (BC); however, the limitations inherent to immunohistochemical analyses prevent comprehensive descriptions of leukocytic infiltrates, as well as evaluation of the functional state of leukocytes in BC stroma. To more fully evaluate this complexity, and to gain insight into immune responses after chemotherapy (CTX), we prospectively evaluated tumor and nonadjacent normal breast tissue from women with BC, who either had or had not received neoadjuvant CTX before surgery. Tissues were evaluated by polychromatic flow cytometry in combination with confocal immunofluorescence and immunohistochemical analysis of tissue sections. These studies revealed that activated T lymphocytes predominate in tumor tissue, whereas myeloid lineage cells are more prominant in “normal” breast tissue. Notably, residual tumors from an unselected group of BC patients treated with neoadjuvant CTX contained increased percentages of infiltrating myeloid cells, accompanied by an increased CD8/CD4 T-cell ratio and higher numbers of granzyme B-expressing cells, compared with tumors removed from patients treated primarily by surgery alone. These data provide an initial evaluation of differences in the immune microenvironment of BC compared with nonadjacent normal tissue and reveal the degree to which CTX may alter the complexity and presence of selective subsets of immune cells in tumors previously treated in the neoadjuvant setting.

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“…S3). In agreement with historic literature (31,32), these results thus indicate that CD68 is not a macrophage-specific marker in human BC.…”

Section: Resultssupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Leukocyte composition of human breast cancer

Ruffell¹,

Rugo

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

405

300

View full text Add to dashboard Cite

show abstract

“…The genes selected for our study cover a broad spectrum of macrophage actions. CD68 is a transmembrane glycoprotein particularly highly expressed in human monocytes and tissue macrophages, participating in lectin/selectin-mediated cell adhesion and locomotion (32). EMR1 is also a transmembrane glycoprotein present in peripheral blood mononuclear cells and presumably involved in cell-cell interactions and activation of consecutive messenger cascades (6).…”

Section: Discussionmentioning

confidence: 99%

Adipose tissue inflammation and liver fat in patients with highly active antiretroviral therapy-associated lipodystrophy

Sevastianova

Sutinen²,

Kannisto³

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

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“…This lysosomal enzyme is usually expressed in granulocytes and macrophages/histiocytes, and indicates the cell's capability for phagocytic (digestive) activity. Therefore, we applied the commercially available monoclonal antibody against the epitope KP-1 of CD 68 (DAKO, Hamburg, Germany) [32,38].…”

Section: Immunohistochemical Staining Proceduresmentioning

confidence: 99%

2002 SSE Award Competition in Basic Science: Expression of major matrix metalloproteinases is associated with intervertebral disc degradation and resorption

et al. 2002

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During the process of degeneration, the intervertebral disc (IVD) shows a progressive and significant reduction in height due to tissue resorption. Intradiscal clefts and tears are major hallmarks of disc degeneration. Matrix-degrading enzymes such as matrix metalloproteinases (MMPs) are assumed to play a pivotal role in disc tissue degradation and resorption. The objective of this study was therefore to investigate the potential role of MMPs in extracellular matrix degradation leading to disc degeneration. This study was conducted on 30 formalin-fixed and EDTA-decalcified complete crosssections of lumbar IVDs from cadavers of individuals aged between 0 and 86 years. Tissue sections were used for the immunolocalization of MMPs-1, -2, -3 and -9. The number of labeled cells was assessed by morphometric analyses, and was statistically correlated with the formation of clefts and tears, cellular proliferation, granular matrix changes and mucous degeneration. Furthermore, 30 disc specimens obtained during spinal surgery were used for in situ hybridization of MMP-2 and -3-mRNA. In addition, the enzymatic gelatinolytic activity was determined by in situ zymography in autopsy material. Immunohistochemistry showed the intradiscal expression of all four MMPs, which was confirmed by in situ hybridization, providing clear evidence for the synthesis of the enzymes within nucleus pulposus and annulus fibrosus cells. Gelatinolytic enzymatic activity was verified by in situ zymography. IVDs from infants and young adolescents remained almost completely unlabeled for all MMPs tested, while more MMPs-1 and -3 were seen in disc cells of younger adults than in those of a more advanced age; MMP-2 remained unchanged over the adult age periods, and MMP-9 was expressed in only relatively few cells. This pattern significantly correlated with the occurrence of clefts and tears. This correlation was strongest for MMP-1 (P<0.0001), MMP-2 (P<0.0017) and MMP-3 (P<0.0005) in the nucleus, and MMP-1 (P<0.0001) and MMP-2 (P<0.038) in the annulus. In parallel, the proliferation of disc cells and matrix degeneration (granular changes and mucous degeneration) were related to MMP expression. Likewise, enzymatic activity was seen in association with cleft formation. Our data suggest that major MMPs play an important role in the degradation of the IVD. This is evidenced by the high correlation of MMP expression with the formation of clefts and tears. These findings implicate a leading function for MMPs in IVD degeneration resulting in the loss of normal disc function, eventually leading to low-back pain.

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Distribution of the CD68 macrophagr/myeloid associated antigen

Cited by 178 publications

References 0 publications

Leukocyte composition of human breast cancer

Leukocyte composition of human breast cancer

Adipose tissue inflammation and liver fat in patients with highly active antiretroviral therapy-associated lipodystrophy

2002 SSE Award Competition in Basic Science: Expression of major matrix metalloproteinases is associated with intervertebral disc degradation and resorption

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