Distribution of the Cereal Cyst Nematodes (Heterodera spp.) in Wheat and Barley Fields in North-Eastern Regions of Syria

Toumi, Fateh; Hassan, Ghassan; Waeyenberge, Lieven; Viaene, Nicole; Dababat, Abdelfattah A.; Nıcol, J. M.; Ogbonnaya, Francis C.; Al-Assas, K.; Al-Fadil, Taissir Abou; Moens, Maurice

doi:10.1007/bf03356561

Cited by 6 publications

(6 citation statements)

References 15 publications

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“…Three surveys conducted in Syria revealed H. latipons to be the most dominant species of the H. avenae group (Abidou et al, 2005b;Hassan, 2008;Toumi et al, 2012). However, the results also revealed that mixtures of two (H. avenae with H. latipons or H. avenae with H. filipjevi) or even three species can occur in a same field.…”

Section: Discussionmentioning

confidence: 99%

Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons

Toumi¹,

Waeyenberge²,

Viaene³

et al. 2013

Nematol

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Several Heterodera species can reduce the yield of wheat and barley, among which H. avenae, H. filipjevi and H. latipons are economically the most important. Their identification, based on morphological characteristics, is not straightforward but can be made easier using molecular techniques. In this study, we developed species-specific primers for the detection of H. latipons. The actin gene of eight Heterodera species was partially sequenced and, after purifying and sequencing the PCR products, all sequences were aligned to find unique sites. The alignment showed moderate to very high similarities between the species. However, a small fragment of the actin gene was suitable for the construction of a potentially useful species-specific primer for H. latipons. The optimised PCR was subsequently tested with several populations of 14 Heterodera species and a single population of Punctodera punctata. Heterodera latipons was represented by 16 populations originating from six different countries. The primer set (Hlat-act), designed using AlleleID 7.73, was shown to be very specific. To test its sensitivity further, the PCR was conducted on DNA extracted from five second-stage juveniles (J2) of H. latipons mixed with five or 100 J2 belonging to H. avenae. The PCR was able to detect up to 1:10 dilution of the DNA obtained from five J2. The results showed that a specific and sensitive H. latipons species-specific PCR was constructed.

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Section: Discussionmentioning

confidence: 99%

Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons

Toumi¹,

Waeyenberge²,

Viaene³

et al. 2013

Nematol

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“…CCNs can seriously reduce yields of cereal crops, especially in temperate and semi-arid regions (Rivoal and Cook, 1993;Nicol and Rivoal, 2008). To keep the population densities of this parasite below damage levels, appropriate management processes, such as crop rotation, are necessary (Mokabli et al, 2002;Smiley et al, 2007), Crop rotations that include broadleaf crops, corn, fallow, and resistant wheat (Toumi et al, 2015), barley, or oat varieties can greatly reduce the nematode density. In addition, growing susceptible hosts less than 50 % of the time in heavy-textured soils and less than 25 % of the time in light-textured soils could dramatically reduce CCN (Yan and Smiley, 2010).…”

Section: Discussionmentioning

confidence: 99%

Incidence and geographical distribution of cereal cyst nematode (CCN, Heterodera spp.) in winter wheat fields in Algeria

Djetti¹,

Hammache²,

Doumandji³

2018

Arx. Misc. Zool.

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Incidence and geographical distribution of the cereal cyst nematode (CCN, Heterodera spp.) in winter wheat fields in Algeria. Cereal cyst nematodes (CCN, Heterodera spp.) are the most damaging plant-parasitic nematode species on wheat, causing severe economic loss in global wheat production. In summer 2015, we analyzed samples collected from 22 wheat fields in Algeria using the Fenwick can technique. The study revealed that 54.55 % of wheat fields were infested with cereal cyst nematodes. The species was observed in several locations in the northern part of Algeria but not in the southern desert area. Population densities of CCNs in soil varied between the regions at an infestation rate of between 0.6 ± 0.54 and 86.6 ± 19.96cysts/500 g of dried soil. Furthermore, we found an average of 56.33 ± 15.18 and 364.70 ± 81.93 second-stage juveniles and eggs per cyst. The infestation was most severe in cereal fields in Draa Semar and Djendel with 86.6 ± 19.96 cyst/500 g of soil and 57.4 ± 17.55 cysts/500 g of soil, respectively. Infestation was lowest in fields in Ras Elouad, Sidi Mbarek and Sedraia with 0.6 ± 0.54 cysts/500 g of soil; 1.6 ± 1.67 cysts/500 g of soil and 2.4 ± 1.67 cysts/500 g of soil, respectively. Heterodera spp. was distributed throughout the cereal growing province in Algeria and could cause economic loss in these regions.

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“…Species distribution maps for representatives of the Avenae group were made using personal communications from colleagues listed in the Acknowledgements and from published sources (Meagher, 1977;Tikhonova, 1978;Abidou et al, 2005b;Holgado et al, 2006;Tanha Maafi et al, 2007;Peng et al, 2009;Pankaj et al, 2011;Dawabah et al, 2012;Mokrini et al, 2012Mokrini et al, , 2017Pridannikov et al, 2015;Toumi et al, 2015a;Li et al, 2016;Tirchi et al, 2016;Cui et al, 2017, and others).…”

Section: Distribution Mapsmentioning

confidence: 99%

DNA barcoding, phylogeny and phylogeography of the cyst nematode species of the Avenae group from the genus Heterodera (Tylenchida: Heteroderidae)

et al. 2018

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SummaryAmong the recognised species groups ofHeterodera, theAvenaegroup is one of the largest with a total of 12 species. Ten of them,H. arenaria,H. aucklandica,H. australis,H. avenae,H. filipjevi,H. mani,H. pratensis,H. riparia,H. sturhaniandH. ustinovi, are morphologically closely related and represent theH. avenaespecies complex, and the other two,H. hordecalisandH. latipons, are morphologically more distinct from this complex. In this study we provide comprehensive phylogenetic analyses of several hundredCOIand ITS rRNA gene sequences from theAvenaegroup using Bayesian inference, maximum likelihood and statistical parsimony. Some 220COIand 11 ITS rRNA new gene sequences from 147 nematode populations collected in 26 countries were obtained in this study. Our study showed that theCOIgene is a powerful DNA barcoding marker for identification of populations and species from theAvenaegroup. A putatively new cyst nematode species related toH. latiponswas revealed from the analysis ofCOIand ITS rRNA gene datasets.COIgene sequences allow distinguishingH. arenaria,H. australisandH. sturhanifrom each other and other species. Problems of species delimiting of these species are discussed. The results of the analysis showed thatCOIhaplotypes corresponded to certain pathotypes of the cereal cyst nematodes. It is recommended that information onCOIhaplotypes of studied populations be included in research with these nematodes. Based on the results of phylogeographical analysis and age estimation of clades with a molecular clock approach, it was hypothesised that several species of theAvenaegroup primarily originated and diversified in the Irano-Anatolian hotspot during the Pleistocene and Holocene periods and then dispersed from this region across the world. Different geographic barriers, centres and times of origin might explain current known distribution patterns for species of theAvenaegroup. Possible pathways, including a long distance trans-Atlantic dispersal, and secondary centres of diversification are proposed and discussed.

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Distribution of the Cereal Cyst Nematodes (Heterodera spp.) in Wheat and Barley Fields in North-Eastern Regions of Syria

Cited by 6 publications

References 15 publications

Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons

Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons

Incidence and geographical distribution of cereal cyst nematode (CCN, Heterodera spp.) in winter wheat fields in Algeria

DNA barcoding, phylogeny and phylogeography of the cyst nematode species of the Avenae group from the genus Heterodera (Tylenchida: Heteroderidae)

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