Distribution of the sulfite resistance gene SSU1-R and the variation in its promoter region in wine yeasts

Yuasa, Noriyuki; Nakagawa, Youji; Hayakawa, Masayuki; Iimura, Yuzuru

doi:10.1016/s1389-1723(04)00303-2

Cited by 47 publications

(35 citation statements)

References 8 publications

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“…Interestingly, a translocation event implying the SSU1 gene with the promoter region of EMC34 has been described for wine yeast [41]. This translocation concerns chromosomes VIII and XVI (VIII-t-XVI) [41], allowing the formation of the so-called SSU1-R allele [57] and increasing sulfite tolerance by enhancing SSU1 expression level [37], [39], [58]. In order to compare the effect of the new XV-t-XVI translocation described here with the VIII-t-XVI and the wild type forms (nt-XVI), lag phase duration was investigated in a synthetic must containing different amounts of total SO 2 (0, 20 and 40 mg/L) using the strains GN (XV-t-XVI), F10 (VIII-t-XVI) and SB (nt-XVI).…”

Section: Resultsmentioning

confidence: 99%

QTL Dissection of Lag Phase in Wine Fermentation Reveals a New Translocation Responsible for Saccharomyces cerevisiae Adaptation to Sulfite

et al. 2014

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Quantitative genetics and QTL mapping are efficient strategies for deciphering the genetic polymorphisms that explain the phenotypic differences of individuals within the same species. Since a decade, this approach has been applied to eukaryotic microbes such as Saccharomyces cerevisiae in order to find natural genetic variations conferring adaptation of individuals to their environment. In this work, a QTL responsible for lag phase duration in the alcoholic fermentation of grape juice was dissected by reciprocal hemizygosity analysis. After invalidating the effect of some candidate genes, a chromosomal translocation affecting the lag phase was brought to light using de novo assembly of parental genomes. This newly described translocation (XV-t-XVI) involves the promoter region of ADH1 and the gene SSU1 and confers an increased expression of the sulfite pump during the first hours of alcoholic fermentation. This translocation constitutes another adaptation route of wine yeast to sulfites in addition to the translocation VIII-t-XVI previously described. A population survey of both translocation forms in a panel of domesticated yeast strains suggests that the translocation XV-t-XVI has been empirically selected by human activity.

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Section: Resultsmentioning

confidence: 99%

QTL Dissection of Lag Phase in Wine Fermentation Reveals a New Translocation Responsible for Saccharomyces cerevisiae Adaptation to Sulfite

et al. 2014

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“…While there is substantial variation in sulfite resistance within and between species (Figure S4), divergence at other loci may be responsible for most differences in sulfite resistance and could compensate for any changes in FZF1 . Within S. cerevisiae , variation in sulfite resistance is associated with a reciprocal translocation upstream of SSU1 that is more frequent in vineyard and wine strains than strains derived from other sources [39], [57]. The inferred loss of sulfite resistance conferred by changes in FZF1 along the lineage leading to S. cerevisiae , combined with the gain of sulfite resistance due to the translocation within some strains of S. cerevisiae , suggests that the evolution of sulfite resistance among species is not simple and compensatory changes may be involved.…”

Section: Discussionmentioning

confidence: 99%

“…Sulfite resistance was also measured for a strain of S. kluyverii and S. bayanus , but the sulfite-dependent delay in growth could not be calculated since the strains grew in the presence of water (control) but not in the presence of sulfite. The wine strain M8 has the known translocation upstream of SSU1 that increases sulfite resistance [39], [57], [74].…”

Section: Supporting Informationmentioning

confidence: 99%

Divergence of the Yeast Transcription Factor FZF1 Affects Sulfite Resistance

Engle

Fay²

2012

PLoS Genet

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Changes in gene expression are commonly observed during evolution. However, the phenotypic consequences of expression divergence are frequently unknown and difficult to measure. Transcriptional regulators provide a mechanism by which phenotypic divergence can occur through multiple, coordinated changes in gene expression during development or in response to environmental changes. Yet, some changes in transcriptional regulators may be constrained by their pleiotropic effects on gene expression. Here, we use a genome-wide screen for promoters that are likely to have diverged in function and identify a yeast transcription factor, FZF1, that has evolved substantial differences in its ability to confer resistance to sulfites. Chimeric alleles from four Saccharomyces species show that divergence in FZF1 activity is due to changes in both its coding and upstream noncoding sequence. Between the two closest species, noncoding changes affect the expression of FZF1, whereas coding changes affect the expression of SSU1, a sulfite efflux pump activated by FZF1. Both coding and noncoding changes also affect the expression of many other genes. Our results show how divergence in the coding and promoter region of a transcription factor alters the response to an environmental stress.

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“…Of the non-Saccharomyces species used in this study, only the non-WGD species Zygosaccharomyces rouxii, T. delbrueckii, L. thermotolerans, and H. vineae have been reportedly isolated from grapes or wine must (Kurtzman et al 2011). Furthermore, sulfites are frequently added to wine must, and wine strains of S. cerevisiae are known to exhibit higher levels of sulfite resistance than nonwine strains (Pérez-Ortín et al 2002; Yuasa et al 2004). Interspecific differ-ences in sulfite resistance (Engle and Fay 2012), copper resistance (Warringer et al 2011), and other environmental conditions that we did not examine may thus contribute to S. cerevisiae's observed dominance of wine fermentations.…”

Section: Ecology Of High-sugar Environmentsmentioning

confidence: 99%

Evolution of ecological dominance of yeast species in high‐sugar environments

2015

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In budding yeasts, fermentation in the presence of oxygen evolved around the time of a whole genome duplication (WGD) and is thought to confer dominance in high-sugar environments because ethanol is toxic to many species. While there are many fermentative yeast species, only Saccharomyces cerevisiae consistently dominates wine fermentations. In this study, we use co-culture experiments and intrinsic growth rate assays to examine the relative fitness of non-WGD and WGD yeast species across environments to assess when S. cerevisiae’s ability to dominate high-sugar environments arose. We show that S. cerevisiae dominates nearly all other non-WGD and WGD species except for its sibling species S. paradoxus in both grape juice and a high-sugar rich medium. Of the species we tested, S. cerevisiae and S. paradoxus have evolved the highest ethanol tolerance and intrinsic growth rate in grape juice. However, the ability of S. cerevisiae and S. paradoxus to dominate certain species depends on the temperature and the type of high-sugar environment. Our results indicate that dominance of high-sugar environments evolved much more recently than the WGD, most likely just prior to or during the differentiation of Saccharomyces species, and that evolution of multiple traits contributes to S. cerevisiae’s ability to dominate wine fermentations.

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Distribution of the sulfite resistance gene SSU1-R and the variation in its promoter region in wine yeasts

Cited by 47 publications

References 8 publications

QTL Dissection of Lag Phase in Wine Fermentation Reveals a New Translocation Responsible for Saccharomyces cerevisiae Adaptation to Sulfite

QTL Dissection of Lag Phase in Wine Fermentation Reveals a New Translocation Responsible for Saccharomyces cerevisiae Adaptation to Sulfite

Divergence of the Yeast Transcription Factor FZF1 Affects Sulfite Resistance

Evolution of ecological dominance of yeast species in high‐sugar environments

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