Distribution of toxic alkaloids in tissues from three herbal medicine Aconitum species using laser micro-dissection, UHPLC–QTOF MS and LC–MS/MS techniques

Jaiswal, Yogini; Liang, Zhitao; Ho, Alan; Wong, Lailai; Peng, Yong; Chen, Hubiao; Zhao, Zhongzhen

doi:10.1016/j.phytochem.2014.07.026

Cited by 34 publications

(25 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metabolites directly reflect the interaction between a plant’s environment and its genome, and metabolic profiling allows further investigation of the impact of plant phenology and biotic or abiotic stressors on plant phenotype [ 2 , 3 , 4 ]. Metabolic profiling is a technique that generates a unique chemical fingerprint for each sample and allows the identification of diverse set of plant secondary products (PSPs) in plant extracts [ 3 , 5 ]. PSPs are defined as metabolites of plant origin that are not related to housekeeping functions of an organism [ 3 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Pyrrolizidine Alkaloids in Foliage of Two Echium spp. Invaders in Australia—A Case of Novel Weapons?

Skoneczny

Weston

Zhu

et al. 2015

IJMS

View full text Add to dashboard Cite

Metabolic profiling allows for simultaneous and rapid annotation of biochemically similar organismal metabolites. An effective platform for profiling of toxic pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) was developed using ultra high pressure liquid chromatography quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry. Field-collected populations of invasive Australian weeds, Echium plantagineum and E. vulgare were raised under controlled glasshouse conditions and surveyed for the presence of related PAs and PANOs in leaf tissues at various growth stages. Echium plantagineum possessed numerous related and abundant PANOs (>17) by seven days following seed germination, and these were also observed in rosette and flowering growth stages. In contrast, the less invasive E. vulgare accumulated significantly lower levels of most PANOs under identical glasshouse conditions. Several previously unreported PAs were also found at trace levels. Field-grown populations of both species were also evaluated for PA production and highly toxic echimidine N-oxide was amongst the most abundant PANOs in foliage of both species. PAs in field and glasshouse plants were more abundant in the more widely invasive species, E. plantagineum, and may provide competitive advantage by increasing the plant’s capacity to deter natural enemies in its invaded range through production of novel weapons.

show abstract

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Pyrrolizidine Alkaloids in Foliage of Two Echium spp. Invaders in Australia—A Case of Novel Weapons?

Skoneczny

Weston

Zhu

et al. 2015

IJMS

View full text Add to dashboard Cite

show abstract

“…The plants belong to the same genus but different species, and a comparative study would help identify the similarities and dissimilarities in their essential oil content and other secondary metabolites. In our previously published studies, we have identified how plant species belonging to different species, but the same genus, can serve as qualitative substitutes for each other based upon their detailed metabolomics analysis [20][21][22][23]. Several studies have reported the asarone content determination and pharmacological studies of Acorus species [24][25][26][27].…”

mentioning

confidence: 99%

“…Apart from the constituents common to both species, the rhizomes of A. calamus were found to contain four other constituents, namely, acetic acid (15), (−)-α-cedrene (16), (9E,11Z,13E)-9,11,13-octadecatrienoic acid (17), and cis-2-octadecenoic acid (18). The whole rhizome tissues of A. tatarinowii exclusively contained four different constituents, namely, squamulosone (19), 9,12-octadecadienoic acid (20), galgravin (21), and (−)-surinamensinol A (22). The metabolites occurring in various tissues were detected by UHPLC-QTOF MS analysis.…”

mentioning

confidence: 99%

“…2S and 3S, Supporting Information, respectively. Seven constituents were found to occur in all the selected tissues of A. calamus; they are 5-hydroxymethylfurfural (1), nonanoic acid (3), calamenene (8), (+)-shyobunone (10), aspidinol (12), 9,12-octadecadienoic acid (20), and (−)-surinamensinol A (22). In addition, certain constituents were found in selected tissues as follows: The epidermis contained α-terpineol (2), methyl eugenol (5), 2-allyl-5-ethoxy-4-methoxyphenol (9), (+)-cedrol (11), and eudesmin (13).…”

mentioning

confidence: 99%

“…In the case of A. tatarinowii, five common constituents were found, namely, 5-hydroxymethylfurfural (1), nonanoic acid (3), calamenene (8), 9,12-octadecadienoic acid (20), and surinamensin (14). In addition, specific metabolites found in target tissues were as follows: The epidermis contained α-terpineol (2), methyl eugenol (5), aspidinol (12), and eudesmin (13).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Metabolite Profiling of Tissues of Acorus calamus and Acorus tatarinowii Rhizomes by Using LMD, UHPLC-QTOF MS, and GC-MS

Jaiswal

Liang

et al. 2015

Planta Med

Self Cite

View full text Add to dashboard Cite

Acorus calamus and its related species are of significant importance to the food and fragrance industries due to their varied applications. They are also a cause of critical concern due to their toxic β-asarone content. Several toxicity cases have occurred due to high β-asarone compositions in food products. Hence, limits for their use are strictly regulated by the Food and Drug Administration, the European Union, and legislations of different countries. The identification of species with a lower β-asarone content is of great significance. In this report, the metabolite profiles and essential oil content of A. calamus and Acorus tatarinowii rhizomes were analysed and compared using UHPLC-QTOF-MS and GC-MS techniques. The metabolite profiles were similar; however, β-asarone content was higher in A. calamus rhizomes. The developed methods can be applied for microscopic and macroscopic identification, and quality control of food products containing β-asarone.

show abstract

Advances of Metabolite Profiling of Plants in Challenging Environments

Sarabia

Hill

Boughton

et al. 2018

Annual Plant Reviews Online

View full text Add to dashboard Cite

Plant metabolism is profoundly involved in physiological regulation and defence responses when the environment is adverse and plant growth and development is negatively affected. Metabolomics techniques allow the characterisation of physiological and biochemical responses to different types of environmental stresses in plants, including drought, salinity, and nutrient deficiencies. Metabolomics analyses of plant systems have been mostly carried out on bulked tissues (i.e. whole roots, leaves, or shoots) which can provide important biological information about plant tolerance and avoidance mechanisms to abiotic stresses. However, individual plant organs and tissues are composed of different kinds of cells which can produce specific metabolic responses to abiotic stress. Thus, recent development and improvement of metabolomics techniques have allowed the analysis of plant metabolic changes in a spatially resolved manner (i.e.in vivometabolomics, cell‐specific metabolomics, and mass spectrometry imaging (MSI)‐based metabolomics). This article provides a comprehensive overview of the recent findings on plant metabolite changes in response to abiotic stress, recent advancements in metabolomics techniques to study plant metabolism, and prospects of MSI‐based plant metabolomics for the study of plant metabolism.

show abstract

Distribution of toxic alkaloids in tissues from three herbal medicine Aconitum species using laser micro-dissection, UHPLC–QTOF MS and LC–MS/MS techniques

Cited by 34 publications

References 43 publications

Metabolic Profiling of Pyrrolizidine Alkaloids in Foliage of Two Echium spp. Invaders in Australia—A Case of Novel Weapons?

Metabolic Profiling of Pyrrolizidine Alkaloids in Foliage of Two Echium spp. Invaders in Australia—A Case of Novel Weapons?

Metabolite Profiling of Tissues of Acorus calamus and Acorus tatarinowii Rhizomes by Using LMD, UHPLC-QTOF MS, and GC-MS

Advances of Metabolite Profiling of Plants in Challenging Environments

Contact Info

Product

Resources

About