Distribution of tropomyosin isoforms in spiny lobster muscles

Ishimoda-Takagi, Tadashi; Itoh, Masaharu; Koyama, Hideki

doi:10.1002/(sici)1097-010x(19970201)277:2<87::aid-jez1>3.0.co;2-5

Cited by 9 publications

(2 citation statements)

References 40 publications

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“…Generally, there are multiple tropomyosin isoforms that expressed by multiple genes and by alternative splicing of transcripts of those genes in many animals [2][3][4][5][6][7][8][9] . Moreover, the isoforms show a tissue-specific distribution in many animals; for example, human 10) , chicken 11) , salmonid fish 12) , lobster 13) , bivalve [14][15][16] , sea urchin 17) , and sea anemone 18) . Although it is likely that tissue-specific distribution is associated with the roles of the isoforms in the tissues, the functions of tropomyosin itself in the absence of troponin are not well known.…”

Section: Introductionmentioning

confidence: 99%

Tissue specificity of tropomyosin isoform in the mussel, Mytilus galloprovincialis

Itoh

Fujinoki

2008

J. Electrophor

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In the mussel, Mytilus galloprovincialis, there were three kinds of tropomyosin isoforms, which were designated as TM1, TM2 and TM3, respectively. It had been reported that TM1 was contained as only tropomyosin isoform and major component in the muscle tissues such as adductor and cardiac muscles, it was confirmed as the major isoform in adductor, cardiac and anterior pedal retractor muscles, and in the mantle and gills. In this present study, meanwhile, TM2 and TM3 were detected as two new isoforms in the mantle and gills. TM2 and TM3 existed only in the mantle and in both the mantle and gills, respectively. Both TM2 and TM3 were minor isoforms compared to TM1. TM2 had almost the same molecular weight as TM1 whereas TM3 had lower molecular weight than TM1. Moreover, TM3 had more basic isoelectric point than TM1 and TM2.

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Section: Introductionmentioning

confidence: 99%

Tissue specificity of tropomyosin isoform in the mussel, Mytilus galloprovincialis

Itoh

Fujinoki

2008

J. Electrophor

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“…Fibre-type-specific isoforms of myosin heavy and light chains, tropomyosin, paramyosin and troponin-I, -T and -C are expressed in lobster and crayfish muscles (Mykles, 1985a,b;Kobayashi et al, 1989;Li and Mykles, 1990;Miyazaki et al, 1990Miyazaki et al, , 1992Miyazaki et al, , 1993Nishita and Ojima, 1990;Garone et al, 1991;Miegel et al, 1992;Cotton and Mykles, 1993;Neil et al, 1993;Galler and Neil, 1994;Sakurai et al, 1996;Ishimoda-Takagi et al, 1997;Cooper et al, 1998;Mykles et al, 1998;Holmes et al, 1999;LaFramboise et al, 1999;Sohn et al, 2000). However, for an isoform to serve as a suitable diagnostic marker, it must (i) be exclusively expressed in a specific fibre type in various decapod crustacean species and (ii) be easily distinguished in SDS-polyacrylamide gels.…”

Section: Introductionmentioning

confidence: 99%

Myofibrillar protein isoform expression is correlated with synaptic efficacy in slow fibres of the claw and leg opener muscles of crayfish and lobster

Mykles

Medler

Koenders

et al. 2002

Journal of Experimental Biology

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SUMMARY In the crayfish and lobster opener neuromuscular preparations of the walking legs and claws, there are regional differences in synaptic transmission even though the entire muscle is innervated by a single excitatory tonic motor neuron. The innervation of the proximal fibres produced larger excitatory postsynaptic potentials (EPSPs) than those of the central fibres. The amplitudes of the EPSPs in the distal fibres were intermediate between those of the proximal and central regions. These differences in EPSP amplitudes were correlated with differences in short-term facilitation between the three regions. When given a 10- or 20-pulse train of stimuli, the proximal fibres showed greater short-term facilitation initially, often followed by a maximization of short-term facilitation towards the end of a train. In contrast, the central fibres showed a linear increase in short-term facilitation throughout a stimulus train. The distal fibres showed intermediate short-term facilitation compared with the other two regions. Analysis of myofibrillar isoforms showed that levels of troponin-T1 (TnT1), a 55 kDa isoform expressed in slow-tonic (S2) fibres, were correlated with synaptic properties. Proximal fibres had the highest levels of TnT1, with lower levels in distal fibres; central fibres lacked TnT1, which is characteristic of slow-twitch (S1) fibres. In addition, differences in troponin-I isoforms correlated with TnT1 levels between the proximal, central and distal regions. The correlation between slow fibre phenotype and strength of innervation suggests a relationship between synaptic structure and expression of troponin isoforms.

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Characterization of muscles associated with the articular membrane in the dorsal surface of the crayfish abdomen

2000

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The anatomy, physiology, and biochemistry of the dorsal membrane muscle (DMA) and the superficial extensor muscle accessory head (SEAcc) in the abdomen of the crayfish, Procambarus clarkii and lobster, Homarus americanus, are reported. These muscles have not been previously characterized physiologically or biochemically. The anatomy was originally described by Pilgrim and Wiersma (1963. J Morph 113:453-587). The arrangement of these muscles varies depending on the abdominal segment. The function of the dorsal membrane muscle is to retract the thin articulating membrane joining the cuticular segments so that the dorsal membrane does not evert during extension of the abdomen. Consequently, the articular membrane does not protrude, and thus potential damage to the membrane is minimized. Examination of nerve terminal morphology revealed strings of varicosities, usually only associated with tonic terminals. The electrophysiological data indicate that there are at least four tonic excitatory and one inhibitory motor neuron innervating these muscles. Facilitation indices and fatigue-resistance indicate physiologically the tonic nature of innervation. Anti-GABA antibodies demonstrate the anatomical presence of an inhibitor motor neuron. The SDS electrophoretic analysis of myofibrillar proteins and Western blots of key protein isoforms for these muscles in crayfish and lobsters also indicate that the DMA and SEAcc muscles are tonic phenotype.

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Distribution of tropomyosin isoforms in spiny lobster muscles

Cited by 9 publications

References 40 publications

Tissue specificity of tropomyosin isoform in the mussel, Mytilus galloprovincialis

Tissue specificity of tropomyosin isoform in the mussel, Mytilus galloprovincialis

Myofibrillar protein isoform expression is correlated with synaptic efficacy in slow fibres of the claw and leg opener muscles of crayfish and lobster

Characterization of muscles associated with the articular membrane in the dorsal surface of the crayfish abdomen

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