Distribution of various types and P25 subtypes of Beet necrotic yellow vein virus in Germany and other European countries

Koenig, R.; Kastirr, Ute; Holtschulte, B.; Deml, G.; Varrelmann, Mark

doi:10.1007/s00705-008-0234-3

Cited by 31 publications

(30 citation statements)

References 23 publications

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“…The third strain group, designated as pathotype P, contains an additional genomic RNA (RNA 5) and was found at first in the region around Pithiviers, France (Koenig et al 1997), later in Kazakhstan (Koenig and Lennefors 2000) and more recently in the UK (Ward et al 2007). Other RNA 5-containing isolates have been reported in Japan, China Kiguchi et al 1996;Miyanishi et al 1999), the UK (Harju et al 2002;Ward et al 2007) and Germany, where one field has been found to be infested by an RNA 5-containing East Asian isolate (Koenig et al 2008). On the basis of sequence variability, isolates containing a fifth RNA molecule have been classified into P-and J-types for the European and Asian isolates respectively .…”

Section: Introductionmentioning

confidence: 95%

Efficiency of Rz1-based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Greece

et al. 2011

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A survey was carried out to investigate the current situation concerning rhizomania disease incidence in sugar beet cultivation of Greece. A systematic field evaluation over locations and years revealed a consistent disease severity pattern according to favourable agroclimatic conditions and pointed to the so far effectiveness of the Rz1 gene-based resistance, as no major disease outbreaks were observed. Molecular analyses aiming at the characterization of the type and genetic diversity of the virus further confirmed the widespread occurrence of BNYVV in the country, as evidenced by RT-PCR amplification of all five known genomic molecules and nested-PCR assays. None of the isolates contained an RNA 5, typically found in pathotype P. On the basis of RFLP patterns, all BNYVV isolates analysed were classified as pathotype A. Sequence determination of the full-length RNA 3-encoded p25 protein, responsible for symptom development, revealed amino acid motifs ACHG/VCHG in the hypervariable region aa [67][68][69][70] . The presence of valine in position 67 did not appear associated with increased pathogenicity and resistance breaking properties, as earlier reported.

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Section: Introductionmentioning

confidence: 95%

Efficiency of Rz1-based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Greece

et al. 2011

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“…J type isolates possess two deletions at amino acid positions 77 and 227-229 in p26, compared to the P type (Schirmer et al, 2005). P type BNYVV only occurs in France, the United Kingdom and Kazakhstan (Koenig et al, 1997;Koenig and Lennefors, 2000;Ward et al, 2007), and the J type mainly occurs in Japan, China and a single field in Germany (Chiba et al, 2011;Li et al, 2008;Koenig et al, 2008).…”

Section: Introductionmentioning

confidence: 96%

“…A and B types contain four RNAs, and the differences at amino acid positions 62, 103 and 172 of the CP gene allowed us to distinguish between A type (T 62 S 103 L 172 ) and B type (S 62 N 103 F 172 ) isolates (Miyanishi et al, 1999). The A type BNYVV has a worldwide distribution (Schirmer et al, 2005), whereas the B type seems to be limited to few areas in Europe (Koenig et al, 2008), Japan (Miyanishi et al, 1999) and China (Li et al, 2008). P and J types possess the fifth RNA (RNA5) and share about 96% sequence identity (Ward et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of beet necrotic yellow vein virus in China

et al. 2015

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“…the A, B and P types, have highly conserved genomes. Nucleotides 199-210 of the P25-coding region, however, show considerable sequence variation, especially in A type BNYVV (Tamada et al, 2003;Meunier et al, 2003;Schirmer et al, 2005;Ward et al, 2007;Kutluk Yilmaz et al, 2007;Li et al, 2008;Koenig et al, 2008). This stretch of nucleotides codes for the P25 aa 67-70, which in the following will be referred to as the 'tetrad'.…”

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A single U/C nucleotide substitution changing alanine to valine in the beet necrotic yellow vein virus P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings

Koenig

Loss

Specht

et al. 2009

Journal of General Virology

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Beet necrotic yellow vein virus (BNYVV) A type isolates E12 and S8, originating from areas where resistance-breaking had or had not been observed, respectively, served as starting material for studying the influence of sequence variations in BNYVV RNA 3 on virus accumulation in partially resistant sugar beet varieties. Sub-isolates containing only RNAs 1 and 2 were obtained by serial local lesion passages; biologically active cDNA clones were prepared for RNAs 3 which differed in their coding sequences for P25 aa 67, 68 and 129. Sugar beet seedlings were mechanically inoculated with RNA 1+2/RNA 3 pseudorecombinants. The origin of RNAs 1+2 had little influence on virus accumulation in rootlets. E12 RNA 3 coding for V 67 C 68 Y 129 P25, however, enabled a much higher virus accumulation than S8 RNA 3 coding for A 67 H 68 H 129 P25. Mutants revealed that this was due only to the V 67 'GUU' codon as opposed to the A 67 'GCU' codon.The Polymyxa betae-transmitted beet necrotic yellow vein virus (BNYVV) is the causal agent of the devastating rhizomania disease. Its genome consists of four, and in some geographical areas five, different RNA species. RNA 1 and 2 contain the genetic information necessary to enable replication, encapsidation, cell-to-cell movement and suppression of RNA silencing in local lesion hosts (Koenig, 2008; Dunoyer et al., 2002). The additional presence of RNA 3, which codes for a 25 kDa protein (P25), is necessary for virus movement in infected sugar beet roots and production of typical rhizomania symptoms. RNA 4 enables efficient vector transmission and suppression of gene silencing in Nicotiana benthamiana roots (Rahim et al., 2007). The severity of symptoms in sugar beet may be enhanced by the additional presence of RNA 5. The three major strain groups of BNYVV, i.e. the A, B and P types, have highly conserved genomes. Nucleotides 199-210 of the P25-coding region, however, show considerable sequence variation, especially in A type BNYVV (Tamada et al., 2003;Meunier et al., 2003;Schirmer et al., 2005;Ward et al., 2007;Kutluk Yilmaz et al., 2007;Li et al., 2008;Koenig et al., 2008). This stretch of nucleotides codes for the P25 aa 67-70, which in the following will be referred to as the 'tetrad'. Also for the sake of brevity we will refer to the variability of the tetrad rather than its coding sequence, although we do not know whether the effects described below are due to changes at the protein or the nucleic acid level. More than 15 variants of the tetrad have been identified so far, raising the question whether these variations influence the pathogenicity of the virus and may be involved in resistance-breaking phenomena. Klein et al. (2007) studied the influence of P25 sequence variation on its oligomerization and pathogenicity for Tetragonia expansa leaves. Chiba et al. (2008) investigated the influence of mutations in the P25 aa 68, 69 and 70 on the local lesion response in mechanically inoculated leaves of lines of Beta maritima and of the sugar beet varieties Rizor and Monomidori. Acosta- ...

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Distribution of various types and P25 subtypes of Beet necrotic yellow vein virus in Germany and other European countries

Cited by 31 publications

References 23 publications

Efficiency of Rz1-based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Greece

Efficiency of Rz1-based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Greece

Genetic diversity and population structure of beet necrotic yellow vein virus in China

A single U/C nucleotide substitution changing alanine to valine in the beet necrotic yellow vein virus P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings

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