Distribution of virulence genes in Aeromonas spp. isolated from Sardinian waters and from patients with diarrhoea

Sechi, Leonardo Antonio; Deriu, Antonella; Falchi, Maria P.; Fadda, Guido; Zanetti, Stefania Anna Lucia

doi:10.1046/j.1365-2672.2002.01522.x

Cited by 76 publications

(63 citation statements)

References 29 publications

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“…The heterogeneity in the distribution of the toxin genes among the Aeromonas spp. have earlier been reported by several workers (Albert et al, 2000;Sechi et al, 2002;Ottaviani et al, 2011) which are in agreement with our findings. All the MPCR positive Aeromonas isolates were positive for at least one toxin gene.…”

Section: Discussionsupporting

confidence: 94%

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Hussain¹,

Jeyasekaran²,

Shakila³

et al. 2013

AJAFST

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A total of 103 representative colonies from 53 healthy and diseased food fish samples were tested for the incidence of Aeromonas spp. Of which 57% of colonies were positive for Aeromonas spp. by the multiplex PCR (MPCR). On an average, the prevalence was found to be 66% with highest incidence in diseased fishes (76%). About 48% of healthy fish samples were observed to contain Aeromonas. The biomoleculoar identification revealed that the selected virulent hemolytic and enterotoxigenic genes appeared in four different patterns viz. alt; act//hlyA/aer; alt and act//hlyA/aer; ast, alt and act//hlyA/aer, among the MPCR positive isolates of Aeromonas spp. The overall prevalence of the toxin genes alt, act/hlyA/aer and ast among the MPCR positive Aeromonas isolates was found to be 89.8%, 72.9% and 20%, respectively. The biochemical confirmation of the MPCR positive Aeromonas isolates revealed different species in the tested samples belonging to A. hydrophila, A. sobria, A. caviae, A. jandaei, A. veronii, A. schubertii and A. trota and the dominant species was found to be A. hydrophila (48%).

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Section: Discussionsupporting

confidence: 94%

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Hussain¹,

Jeyasekaran²,

Shakila³

et al. 2013

AJAFST

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“…Previous clinical studies [32,33] reported that the expression of multiple biological activities, as in the case of the AHCYTOEN gene, is necessary for the expression of microbial pathogenicity. This cytolytic enterotoxin gene is considered a characteristic virulence trait in Aeromonas [3,34] and its general presence in A. culicicola, indicates that this species may have public health significance. The presence of hemolysin genes in isolates shows that it is a virulent strain and as a result infection in human by contacts during handling of fish.…”

Section: Discussionmentioning

confidence: 99%

Detection of 232bp Virulent Gene of Pathogenic <i>Aeromonas hydrophila</i> through PCR Based Technique: (<i>A Rapid Molecular Diagnostic Approach</i>)

Sarkar¹,

Saha²,

Roy³

2013

AiM

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The pathogenicity of aeromonads produces due to exotoxins such as cytolytic enterotoxin, hemolysin or aerolysin, lipases and proteases. Rapid detection of A. hydrophila cytolytic enterotoxin (AHCYTONE) gene and their characterization has proven importance so that proper and rapid preventive and control measures could be taken up to reduce mortality and loss in fish culture. The main objective of the present study is to genetic identification of AHCYTONE positive Aeromonas hydrophila. Strains were isolated from fishes from different fish market in West Bengal and water samples from different river and ponds. Initially strains were identified by their phenotypic and biochemical characterization. Due to contradiction of those results, molecular characterization was done by polymerase chain reaction, which is proved a suitable and rapid diagnostic tool for identification and characterization of A. hydrophila. We have also evaluated the potential risk to human health that this finding can represent by determining the presence of the cytolytic enterotoxin gene in such isolates.

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“…Many studies have investigated the genetic diversity of human diarrhea and environmental isolates of A. hydrophila (Aguilera-Arreola et al, 2007;Sechi et al, 2002;Szczuka and Kaznowski, 2004). All these studies revealed a high intra-specific genetic diversity within A. hydrophila, and no dominant clones were found.…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence typing revealed a clonal lineage of Aeromonas hydrophila caused motile Aeromonas septicemia outbreaks in pond-cultured cyprinid fish in an epidemic area in central China

Zhang

Yang

et al. 2014

Aquaculture

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Distribution of virulence genes in Aeromonas spp. isolated from Sardinian waters and from patients with diarrhoea

Cited by 76 publications

References 29 publications

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Detection of 232bp Virulent Gene of Pathogenic <i>Aeromonas hydrophila</i> through PCR Based Technique: (<i>A Rapid Molecular Diagnostic Approach</i>)

Multilocus sequence typing revealed a clonal lineage of Aeromonas hydrophila caused motile Aeromonas septicemia outbreaks in pond-cultured cyprinid fish in an epidemic area in central China

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Distribution of virulence genes in Aeromonas spp. isolated from Sardinian waters and from patients with diarrhoea

Cited by 76 publications

References 29 publications

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Detection of 232bp Virulent Gene of Pathogenic &lt;i&gt;Aeromonas hydrophila&lt;/i&gt; through PCR Based Technique: (&lt;i&gt;A Rapid Molecular Diagnostic Approach&lt;/i&gt;)

Multilocus sequence typing revealed a clonal lineage of Aeromonas hydrophila caused motile Aeromonas septicemia outbreaks in pond-cultured cyprinid fish in an epidemic area in central China

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Detection of 232bp Virulent Gene of Pathogenic <i>Aeromonas hydrophila</i> through PCR Based Technique: (<i>A Rapid Molecular Diagnostic Approach</i>)