Distribution Pattern of γ-amino Butyric Acid Immunoreactive Neural Structures in the Central and Peripheral Nervous System of the Tubificid Worm, Limnodrilus hoffmeisteri

Molńar, László; Kiszler, Gábor; Pollák, Edit; Deres, László

doi:10.1007/s10750-005-1705-0

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…They contain various neuroactive compounds like dopamine [20,23], serotonin, histamine, GABA and some neuropeptides, e.g. proctolin, FVRIamide and Eisenia tetradecapeptide [2,17,19] indicating that the sensory system operates with various transmitters [2].…”

Section: Introductionmentioning

confidence: 99%

NADPH-diaphorase histochemistry selectively stains peripheral and central sensory structures of lumbricid earhworms

Zsombok¹,

Pollák²,

Molńar³

2016

Acta Biologica Hungarica

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By means of whole mount NADPH-diaphorase histochemistry the distribution pattern of primary sensory cells (PSC) and the pathway of their central processes in the ventral nerve cord (VNC) ganglia were investigated in the lumbricid earthworms, Eisenia fetida and Lumbricus terrestris. The distribution pattern of the stained structures seemed to be the same in both species investigated. Strong labelling occurred in sensory fibre branches of segmental nerves and in each of the sensory longitudinal axon bundles of VNC ganglia. Based on their anatomical location some NADPH-d positive central sensory cells were identified from among which the putative tactile receptors were characterized by constant, strong staining.

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Section: Introductionmentioning

confidence: 99%

NADPH-diaphorase histochemistry selectively stains peripheral and central sensory structures of lumbricid earhworms

Zsombok¹,

Pollák²,

Molńar³

2016

Acta Biologica Hungarica

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Identification of novel neuropeptides in the ventral nerve cord ganglia and their targets in an annelid worm, Eisenia fetida

Herbert¹,

Pollák

Zougman

et al. 2009

J of Comparative Neurology

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Periviscerokinins (PVKs) and pyrokinins (PKs) are neuropeptides known in several arthropod species. Sequence homology of these peptides with the molluscan small cardioactive peptides reveals that the occurrence of PVKs and PKs is not restricted to arthropods. Our study focuses on the biochemical and immunocytochemical identification of neuropeptides with sequence homology to PVKs and PKs in the central and peripheral nervous system of the earthworm Eisenia fetida. By means of affinity chromatography, nanoflow liquid chromatography, and high accuracy mass spectrometry, six peptides, SPFPR(L/I)amide, APFPR(L/I)amide, SPLPR(L/I)amide, SFVR(L/I)amide, AFVR(L/I)amide, and SPAFVR(L/I)amide, were identified in the central nervous system with the common -XR(L/I)amide C-terminal sequence. The exact anatomical position of 13 labeled XR(I/L)amide expressing neuron groups and numerous peptide-containing fibers were determined by means of immunocytochemistry and confocal laser scanning microscopy in whole-mount preparations of ventral nerve cord ganglia. The majority of the stained neurons were interneurons with processes joining the distinct fine-fibered polysegmental tracts in the central neuropil. Some stained fibers were seen running in each segmental nerve that innervated metanephridia and body wall. Distinct groups of neurosecretory cells characterized by small round soma and short processes were also identified. Based on immunoelectron microscopy six different types of labeled cells were described showing morphological heterogeneity of earthworm peptides containing elements. Our findings confirm that the sequence of the identified earthworm neuropeptides homologous to the insect PVKs and PKs suggesting that these peptides are phylogenetically conservative molecules and are expressed in sister-groups of animals such as annelids, mollusks, and insects.

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Organization of the sensory system of the earthworm Lumbricus terrestris (Annelida, Clitellata) visualized by DiI

Kiszler

Varhalmi

Berta

et al. 2012

Journal of Morphology

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The anatomical organization of the peripheral and central sensory structures of the earthworm Lumbricus terrestris was investigated applying a fluorescent carbocyanine dye (DiI) as a neuronal tracer. Using whole-mount preparations and confocal laser scanning microscopy, the pattern of primary sensory cells and pathways of their processes were traced and reconstructed in three-dimensions. Our study shows that a ventral nerve cord ganglion receives sensory fibers from at least two adjacent segments suggesting that the peripheral nervous system is not segmental in its arrangement and the receptive-fields of the body wall overlap in earthworms. Furthermore, our result suggests an integrative function of the basiepidermal plexus consists of sensory and motor fibers.

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Distribution Pattern of γ-amino Butyric Acid Immunoreactive Neural Structures in the Central and Peripheral Nervous System of the Tubificid Worm, Limnodrilus hoffmeisteri

Cited by 3 publications

References 40 publications

NADPH-diaphorase histochemistry selectively stains peripheral and central sensory structures of lumbricid earhworms

NADPH-diaphorase histochemistry selectively stains peripheral and central sensory structures of lumbricid earhworms

Identification of novel neuropeptides in the ventral nerve cord ganglia and their targets in an annelid worm, Eisenia fetida

Organization of the sensory system of the earthworm Lumbricus terrestris (Annelida, Clitellata) visualized by DiI

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