Distribution ratio, distribution constant and partition coefficient. Countercurrent chromatography retention of benzoic acid

Preparative separation of glycoalkaloids α-solanine and α-chaconine by centrifugal partition chromatography

“…The amphiphilic complex nature of our sample can cause a significant stationary phase loss during the run and/or can disturb the mass transfer. In support of this explanation in relation to the chemical structure of the studied compounds, Berthod et al recently showed a discrepancy between the measured and calculated separation factors during the separation of benzoic acid that could be explained by the specific nature of the compound [19].…”

Separation and Purification with a Liquid Stationary Phase

“…In a recent study, we showed that the tabulated heptane/water benzoic acid partition coefficient was 0.2 at 20 • C. In CCC experiments, the observed benzoic acid K values could be as low as 0 with high pH aqueous mobile phases, because benzoic acid was carried as benzoate anion, rather than being retained by the heptane stationary phase. The benzoic acid experimental CCC K value could also be as high as 25 with an acidic mobile phase and benzoic acid dimerization in the heptane phase [12].…”

A strategy for the separation of diterpenoid isomers from the root of Aralia continentalis by countercurrent chromatography: The distribution ratio as a substitute for the partition coefficient and a three-phase solvent system

“…Berthod et al as the total concentration of a solute in the stationary phase in all possible forms over the total concentration of the solute in the mobile phase in all its possible forms [5].…”