Disturbance of perineuronal nets in the perilesional area after photothrombosis is not associated with neuronal death

Karetko-Sysa, M; Skangiel-Kramska, J; Nowicka, Dorota

doi:10.1016/j.expneurol.2011.05.022

Cited by 41 publications

(39 citation statements)

References 87 publications

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“…Indeed, some discordance between the two PNN markers staining has been observed previously Karetko-Sysa et al, 2011). Moreover, it appears that they can be regulated differentially in response to various stimuli, e.g., sensory deprivation (McRae et al, 2007) or ischemic stroke (Karetko-Sysa et al, 2011). It has been shown by Dino et al (2006) that, apart from aggrecan-associated, CAT-315-binding epitope is also detected on membrane-bound RPTPb (Receptor Tyrosine Phosphatase b) molecule and its soluble, PNN-associated isoform, phosphacan.…”

Section: Discussionmentioning

confidence: 67%

“…According to previous studies, both WFA-and CAT-315-binding epitopes are localized on the aggrecan molecule; however, they potentially correspond to two different sugars (Matthews et al, 2002). Indeed, some discordance between the two PNN markers staining has been observed previously Karetko-Sysa et al, 2011). Moreover, it appears that they can be regulated differentially in response to various stimuli, e.g., sensory deprivation (McRae et al, 2007) or ischemic stroke (Karetko-Sysa et al, 2011).…”

Section: Discussionmentioning

confidence: 92%

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Aging somatosensory cortex displays increased density of WFA-binding perineuronal nets associated with GAD-negative neurons

2014

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 92%

Aging somatosensory cortex displays increased density of WFA-binding perineuronal nets associated with GAD-negative neurons

2014

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“…PNN degradation with the bacterial enzyme Chondroitinase ABC restored synaptic plasticity in the visual cortex after the critical period (Pizzorusso et al, 2002). Although single components like Neurocan can be upregulated after damage (Kwok et al, 2011), reduction of PNNs has been described, which is in line with the fact that plasticity is increased under this condition (Karetko-Sysa et al, 2011). This shows that not only the presence of molecules as such is important, but also the spatial distribution and the interaction of different factors.…”

Section: Extracellular Signals Influence Regeneration and Stem/progenmentioning

confidence: 82%

Influence of the extracellular matrix on endogenous and transplanted stem cells after brain damage

Roll

Faissner

2014

Front. Cell. Neurosci.

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The limited regeneration capacity of the adult central nervous system (CNS) requires strategies to improve recovery of patients. In this context, the interaction of endogenous as well as transplanted stem cells with their environment is crucial. An understanding of the molecular mechanisms could help to improve regeneration by targeted manipulation. In the course of reactive gliosis, astrocytes upregulate Glial fibrillary acidic protein (GFAP) and start, in many cases, to proliferate. Beside GFAP, subpopulations of these astroglial cells coexpress neural progenitor markers like Nestin. Although cells express these markers, the proportion of cells that eventually give rise to neurons is limited in many cases in vivo compared to the situation in vitro. In the first section, we present the characteristics of endogenous progenitor-like cells and discuss the differences in their neurogenic potential in vitro and in vivo. As the environment plays an important role for survival, proliferation, migration, and other processes, the second section of the review describes changes in the extracellular matrix (ECM), a complex network that contains numerous signaling molecules. It appears that signals in the damaged CNS lead to an activation and de-differentiation of astrocytes, but do not effectively promote neuronal differentiation of these cells. Factors that influence stem cells during development are upregulated in the damaged brain as part of an environment resembling a stem cell niche. We give a general description of the ECM composition, with focus on stem cell-associated factors like the glycoprotein Tenascin-C (TN-C). Stem cell transplantation is considered as potential treatment strategy. Interaction of transplanted stem cells with the host environment is critical for the outcome of stem cell-based therapies. Possible mechanisms involving the ECM by which transplanted stem cells might improve recovery are discussed in the last section.

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“…At day 3 after stroke, further ongoing cell death and increase of infarct size is not likely. [20], [21] Therefore, day 3 is frequently used as a time-point to initiate neuroregenerative therapies in experimental studies. Additionally, although PT has been shown to be responsive to neuroprotectants, [22], [23] it is much less responsive than other stroke models [24], [25].…”

Section: Discussionmentioning

confidence: 99%

FTY720 Treatment in the Convalescence Period Improves Functional Recovery and Reduces Reactive Astrogliosis in Photothrombotic Stroke

et al. 2013

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BackgroundThe Sphingosine-1-phosphate (S1P) signaling pathway is known to influence pathophysiological processes within the brain and the synthetic S1P analog FTY720 has been shown to provide neuroprotection in experimental models of acute stroke. However, the effects of a manipulation of S1P signaling at later time points after experimental stroke have not yet been investigated. We examined whether a relatively late initiation of a FTY720 treatment has a positive effect on long-term neurological outcome with a focus on reactive astrogliosis, synapses and neurotrophic factors.MethodsWe induced photothrombotic stroke (PT) in adult C57BL/6J mice and allowed them to recover for three days. Starting on post-stroke day 3, mice were treated with FTY720 (1 mg/kg b.i.d.) for 5 days. Behavioral outcome was observed until day 31 after photothrombosis and periinfarct cortical tissue was analyzed using tandem mass-spectrometry, TaqMan®analysis and immunofluorescence.ResultsFTY720 treatment results in a significantly better functional outcome persisting up to day 31 after PT. This is accompanied by a significant decrease in reactive astrogliosis and larger post-synaptic densities as well as changes in the expression of vascular endothelial growth factor α (VEGF α). Within the periinfarct cortex, S1P is significantly increased compared to healthy brain tissue.ConclusionBesides its known neuroprotective effects in the acute phase of experimental stroke, the initiation of FTY720 treatment in the convalescence period has a positive impact on long-term functional outcome, probably mediated through reduced astrogliosis, a modulation in synaptic morphology and an increased expression of neurotrophic factors.

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Disturbance of perineuronal nets in the perilesional area after photothrombosis is not associated with neuronal death

Cited by 41 publications

References 87 publications

Aging somatosensory cortex displays increased density of WFA-binding perineuronal nets associated with GAD-negative neurons

Aging somatosensory cortex displays increased density of WFA-binding perineuronal nets associated with GAD-negative neurons

Influence of the extracellular matrix on endogenous and transplanted stem cells after brain damage

FTY720 Treatment in the Convalescence Period Improves Functional Recovery and Reduces Reactive Astrogliosis in Photothrombotic Stroke

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