Disturbed sugar metabolism in a fully habituated nonorganogenic callus of Beta vulgaris (L.)

Bisbis, Badia; Dily, Frédérik Le; Kevers, Claire; Billard, J. P.; Huault, C.; Gaspar, Thomas

doi:10.1007/bf00024846

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…The origin of the obviously greater reducing power of H cells, as compared to N cells, must be found within the cells. In a attempt to understand this origin, we have tested several dehydrogenase activities able to produce reducing equivalents in the form of NAD(P)H. It appeared that, in addition to glutamate dehydrogenase (Le ), 6-phosphogluconate dehydrogenase and glucose-6-phopsphate dehydrogenase (Bisbis et al 1993), which were already reported to be more active in H cells, malate dehydrogenase was a possible candidate, being clearly more active in H cells (Fig. 2).…”

Section: Discussionmentioning

confidence: 99%

Redox characteristics of normal and habituated cell lines of sugarbeet

Carrié

Gaspar

Greppin

et al. 1994

Plant Cell & Environment

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Redox properties of cells of normal hormone-requiring (N) and habituated (H) sugarbeet calli have been investigated. It was found that H cells at all ages reduced exogenous ferricyanide at a much higher rate than N cells, but they exhibited a lower chemiluminescence either alone or in presence of luminol. The plasma membrane NADH:ferricyanide oxidoreductase measured in vitro was almost identical for the two cell types. This could indicate that the higher reducing power of H cells had an intracellular origin. Among the enzyme activities which could provide electrons, malate dehydrogenase activity was found to be a good candidate, being more active in H cells. The results are discussed in relation to the abnormal structure of the cell wall of habituated cells.

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Section: Discussionmentioning

confidence: 99%

Redox characteristics of normal and habituated cell lines of sugarbeet

Carrié

Gaspar

Greppin

et al. 1994

Plant Cell & Environment

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“…Cells of this callus furthermore exhibit many features of cancerous cells (Gaspar et al 1991): monoclonal origin (Kevers et al 1981), abnormally shaped large polyploid nuclei with several nucleoli (Hagtge et al 1992a), disturbed sugar metabolism (Bisbis et al 1993) and deviated nitrogen metabolism leading to polyamine accumulation (Hagtge et aL 1990, Le Dily et al 1993a), elevated levels of diacylglycerol and inositol phosphates (Feutry et ai., unpublished). These poorly differentiated cells also exhibit incompletely structured plastids and mitochondria (Crevecoeur et al 1992) with a general deficiency of the tetrapyrrole-compounds, peroxidase, catalase, and cytochromes besides Chl (Hag~ge et al 1992b, Le Dily et al 1993b.…”

Section: Introductionmentioning

confidence: 99%

Chlorophylls and carotenoids in a fully habituated nonorganogenic callus ofBeta vulgaris

Bisbis¹,

Dujardin²,

Kevers³

et al. 1994

Biologia plant.

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A fully habituated (H) nonorganogenic sugar beet callus, subcultured in the light, did not contain detectable chlorophyll (Chl) nor carotenoid (Car). It accumulated some Car in the dark. Fluorescence spectra indicated that this H callus also accumulated some protochlorophyllide which, however, was not well integrated into the protochlorophyllide-NADPH-photoreductase complex, and therefore not transformed into chlorophyllide in the light. The H callus showed no variable fluorescence which indicated absence of photosynthesis, and therefore it suggested a full heterotrophic behaviour of this peculiar callus line. A green hormone-dependent callus of the same sugar beet had normal fluorescence spectra and kinetics comparable to those of a green leaf.

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Biochemical comparisons of habituated and non-habituated callus lines of Glycine max (L.) cv. Acme

et al. 1996

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A cytokinin habituated non-organogenic callus line of Glycine max L. cv. Acme, was isolated. Cytokinins were detected in both non-and habituated calli. HPLC analysis indicated that the highest biological activity was associated with compounds that cochromatographed with zeatin and derivatives thereof. Cytokinin-like activity in the habituated callus was higher than in the non-habituated callus. No difference was observed in the ability of the two callus types to metabolise rrans-zeatin. The habituated callus however, metabolised N6(A2-isopentenyl)adenine (iP)at a slower rate than the non-habituated line. The regulation of proline and putrescine levels during the growth cycle was similar in both callus types, although less proline was present in the habituated than the non-habituated callus. In both callus types a decrease in putrescine levels was accompanied by an increase in the proline levels on day 21 of the growth cycle. Neither spermine nor spermidine were detected in either the habituated or nonhabituated lines. A comparison of the total RNA levels of the habituated and non-habituated material revealed higher RNA levels in the non-habituated than the habituated line. Two-dimensional gel electrophoresis indicated the presence of 3 polypeptides in the habituated callus on day 13 of the growth cycle. These were not detected in the non-habituated callus, of similar age.

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Disturbed sugar metabolism in a fully habituated nonorganogenic callus of Beta vulgaris (L.)

Cited by 8 publications

References 15 publications

Redox characteristics of normal and habituated cell lines of sugarbeet

Redox characteristics of normal and habituated cell lines of sugarbeet

Chlorophylls and carotenoids in a fully habituated nonorganogenic callus ofBeta vulgaris

Biochemical comparisons of habituated and non-habituated callus lines of Glycine max (L.) cv. Acme

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