DiSUMO-like DSUL is required for nuclei positioning, cell specification and viability during female gametophyte maturation in maize

Srilunchang, Kanok-orn; Krohn, Nádia Graciele; Dresselhaus, Thomas

doi:10.1242/dev.035964

Cited by 44 publications

(40 citation statements)

References 73 publications

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“…In FG mutants with abnormally positioned or extra nuclei in maize (Evans, 2007;Srilunchang et al, 2010) and Arabidopsis (Gross-Hardt et al, 2007;Johnston et al, 2010;Moll et al, 2008;Pagnussat et al, 2007), the nuclei acquire their fate according to their spatial positioning along the micropylar-chalazal axis, suggesting that cell specification in the FG is driven by positional information. This hypothesis was strengthened by a microscopy study showing a graded activity of the auxin-sensitive reporter DR5 (Ottenschläger et al, 2003) along the micropylar-chalazal axis of the FG (Pagnussat et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Theoretical and experimental evidence indicates that there is no detectable auxin gradient in the angiosperm female gametophyte

et al. 2013

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The plant life cycle alternates between a diploid sporophytic and a haploid gametophytic generation. The female gametophyte (FG) of flowering plants is typically formed through three syncytial mitoses, followed by cellularisation that forms seven cells belonging to four cell types. The specification of cell fates in the FG has been suggested to depend on positional information provided by an intrinsic auxin concentration gradient. The goal of this study was to develop mathematical models that explain the formation of this gradient in a syncytium. Two factors were proposed to contribute to the maintenance of the auxin gradient in Arabidopsis FGs: polar influx at early stages and localised auxin synthesis at later stages. However, no gradient could be generated using classical, one-dimensional theoretical models under these assumptions. Thus, we tested other hypotheses, including spatial confinement by the large central vacuole, background efflux and localised degradation, and investigated the robustness of cell specification under different parameters and assumptions. None of the models led to the generation of an auxin gradient that was steep enough to allow sufficiently robust patterning. This led us to re-examine the response to an auxin gradient in developing FGs using various auxin reporters, including a novel degron-based reporter system. In agreement with the predictions of our models, auxin responses were not detectable within the FG of Arabidopsis or maize, suggesting that the effects of manipulating auxin production and response on cell fate determination might be indirect.

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Section: Introductionmentioning

confidence: 99%

Theoretical and experimental evidence indicates that there is no detectable auxin gradient in the angiosperm female gametophyte

et al. 2013

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“…Until recently, there were only a few specific promoters defined in maize (Cao et al, 2007, Sattarzadeh et al, 2009, Srilunchang et al, 2010, Zhang et al, 2009. We generated a set of promoter-FP direct fusion marker lines, which can be used for FACS experiments.…”

Section: Application Of Fluorescent Protein Marker Lines In Cell Specmentioning

confidence: 99%

Fluorescent protein marker lines in maize: generation and applications

Wu¹,

Luo²,

Zadrozny³

et al. 2013

Int. J. Dev. Biol.

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Fluorescent proteins (FP) have significantly impacted the way that we study plants in the past two decades. In the post-genomics era, these FP tools are in higher demand by plant scientists for studying the dynamics of protein localization, function, and interactions, and to translate sequence information to biological knowledge that can benefit humans. Although FP tools have been widely used in the model plant Arabidopsis, few FP resources have been developed for maize, one of the most important food crops worldwide, and an ideal species for genetic and developmental biology research. In an effort to provide the maize and cereals research communities with a comprehensive set of FP resources for different purposes of study, we generated more than 100 stable transformed maize FP marker lines, which mark most compartments in maize cells with different FPs. Additionally, we are generating driver and reporter lines, based on the principle of the pOp-LhG4 transactivation system, allowing specific expression or mis-expression of any gene of interest to precisely study protein functions. These marker lines can be used not only for static protein localization studies, but will be useful for studying protein dynamics and interactions using kinetic microscopy methods, such as fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), and fluorescence resonance energy transfer (FRET). All of the constructs and maize marker lines are publicly available through our website, http://maize. jcvi.org/cellgenomics/index.php

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“…The gene represented 17 by the EST sequence EPS87 (TaDSUL) codes for a protein with small ubiquitin-like modifier 18 (SUMO) and ubiquitin-like domains (di-SUMO-like or DSUL, gene bank accession: 19 ACL50300.1). Recently, the maize homologue of this protein has been characterized in detail 20 by Srilunchang et al (Srilunchang et al 2010) who specified that it is required for nuclei 21 positioning, cell specification and viability during female gametophyte maturation. 22…”

Section: Est Sequencing 12mentioning

confidence: 99%

“…The maize homologue of TaDSUL, a 1 protein may be involved in the post-translational regulation of other proteins by sumoylation 2 during female gametophyte maturation (Srilunchang et al 2010). This gene was reported to 3 be exclusively expressed in the micropylar region of the immature female gametophyte, while 4 after cellularization its expression was restricted to the egg cell and the zygote (Srilunchang et 5 al.…”

Section: And the 24mentioning

confidence: 99%