Diuretic effect of the aqueous extract of Bidens odorata in the rat

Meléndez-Camargo, María Estela; Berdeja, Blanca; Miranda, Guadalupe Aleida Valenzuela

doi:10.1016/j.jep.2004.08.005

Cited by 20 publications

(5 citation statements)

References 9 publications

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“…The animals were observed for overt toxicities like diarrhea, weight loss, tremor, lethargy and paralysis periodically for the first four hours during the 24 h period and then followed for 14 days for any lethality [12]. …”

Section: Methodsmentioning

confidence: 99%

Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice

Hailu

Engidawork

2014

BMC Complement Altern Med

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BackgroundIn the Ethiopian traditional medicine, the leaves of Ajuga remota B. (Local name, Armagusa) is used in the treatment of hypertension. Since this claim has not been investigated scientifically, the aim of the present study was to evaluate the diuretic potential of the aqueous and 80% methanol extracts of the leaves of Ajuga remota in mice after acute oral administration.MethodsAdult mice were administered orally either aqueous (250 mg/kg, AA250; 500 mg/kg, AA500 and 1000 mg/kg, AA1000) or 80% methanol (250 mg/kg, AM250; 500 mg/kg, AM500 and 750 mg/kg, AM750) extract. Urine output and electrolyte contents were then quantified up to 5 h and compared with those administered with furosemide 10 mg/kg (F10) and distilled water (CON).ResultsThe larger dose of 80% methanol extract produced significant diuresis (p < 0.01), while the aqueous extract had shown diuresis both at the middle (p < 0.01) and higher (p < 0.01) doses by the end of the fifth hour compared to CON mice. Regarding electrolyte excretion, larger doses of both extracts had increased natriuresis (p < 0.001 for AA1000 and p < 0.01 for AM1000), while the effect on kaliuresis were smaller when compared with the standard, suggesting the plant could possibly have a potassium-sparing effect. Phytochemical screening revealed the presence of secondary metabolites like phenolic compounds, tannins, saponins, flavonoids, terpenoids, steroids, and cardiac glycosides, which might account for the diuretic activity.ConclusionsThe results indicate that the plant is endowed with significant diuretic activity at various doses, providing evidence for its folkloric use. The major components like flavonoids, tannins, terpenoids and alkaloids found in the plant might have contributed to the observed diuretic activity.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice

Hailu

Engidawork

2014

BMC Complement Altern Med

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“…Among them, Bidens odorata Cav., commonly known as “Mozoquelite” or “Aceitilla,” is used to treat gastrointestinal discomforts such as diarrhea [ 2 , 3 ], as well as headaches and pain of the lumbar region [ 2 , 4 ]. It has also been found that aqueous extracts of this plant have a diuretic effect [ 5 ]. However, there have been no reports regarding the antidiarrheal activity of this plant.…”

Section: Introductionmentioning

confidence: 99%

Composition and Antidiarrheal Activity ofBidens odorataCav.

Zavala-Mendoza¹,

Alarcón-Aguilar

Pérez-Gutiérrez

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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The antidiarrheal effects of chloroform, methanol, and aqueous extracts of Bidens odorata Cav. were investigated at doses of 200 mg/kg on castor-oil-induced diarrhea. The chloroform extract of B. odorata (CBO) reduced diarrhea by 72.72%. The effect of CBO was evaluated on mice with diarrhea induced by castor oil, MgSO4, arachidonic acid, or prostaglandin E2. CBO inhibited the contraction induced by carbachol chloride on ileum (100 µg/mL) and intestinal transit (200 mg/kg) in Wistar rats. The active fraction of CBO (F4) at doses of 100 mg/kg inhibited the diarrhea induced by castor oil (90.1%) or arachidonic acid (72.9%) but did not inhibit the diarrhea induced by PGE2. The active fraction of F4 (FR5) only was tested on diarrhea induced with castor oil and inhibited this diarrhea by 92.1%. The compositions of F4 and FR5 were determined by GC-MS, and oleic, palmitic, linoleic, and stearic acids were found. F4 and a mixture of the four fatty acids inhibited diarrhea at doses of 100 mg/kg (90.1% and 70.6%, resp.). The results of this study show that B. odorata has antidiarrheal effects, as is claimed by folk medicine, and could possibly be used for the production of a phytomedicine.

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“…Two methods were used to prepare extracts from the air-dried plant samples: (i) total extract was obtained from 10 g samples of the B. pilosa whole plant. This material was dissolved in 1,000 mL of boiling distilled water, lightly stirred and covered with gauze for 10 min, recovered by filtration, and the mixture was cooled to room temperature, as described (22, 23); (ii) acetonic fraction of B. pilosa was obtained by precipitation process of the total extract by adding cold acetone (1:1) and incubated for 30 min at −70°C, followed by 15 min at −20°C. After centrifugation at 14,000 × g for 20 min at 4°C, the supernatant was discarded and the precipitate was solubilized in 0.9% NaCl.…”

Section: Methodsmentioning

confidence: 99%

Acetonic Fraction of Bidens pilosa Enriched for Maturase K Is Able to Control Cerebral Parasite Burden in Mice Experimentally Infected With Toxoplasma gondii

et al. 2019

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Toxoplasma gondii infection can cause abortions or congenital infection for a vast number of domestic animals and humans, leading to economic loss in veterinary sciences, as well as severe consequences for immunocompromised patients. Bidens pilosa Linné has been used in ethnopharmacology for treatment of diseases, as malaria, diabetes and hepatitis, in addition to its use as antioxidant, antiallergic, anti-inflammatory, and antiviral. The components of this plant have never been studied before for treatment of toxoplasmosis, and the conventional drugs currently used to treat this disease have high degree of toxicity. Thus, the aim of this study was to evaluate the effect of B. pilosa against T. gondii, by analyzing a total extract of this plant in parallel with a fraction obtained by precipitation in acetone. Also, it was assessed if the acetonic fraction could present lectinic activity, followed by its identification by mass spectrometry. It was observed with the experimental models designed that both total extract and acetonic fraction of B. pilosa were able to control T. gondii infection by in vitro and in vivo experiments, in addition to their low toxicity to host cells. Both total extract and acetonic fraction of this plant display capacity to impair replication of T. gondii tachyzoites. Interesting, the B. pilosa acetonic fraction treatment for 10 days after infection decreases significantly the number of T. gondii brain cyst in comparison with controls. The protein isolated from B. pilosa acetonic fraction was characterized as a novel lectin identified as maturase K. Taken together, these findings open new perspectives to treat patients infected by T. gondii. Future studies will be necessary to investigate the precise mechanism underlying the control of T. gondii infection to impair the replication of this parasite in the host cells after treatment with B. pilosa maturase K.

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Diuretic effect of the aqueous extract of Bidens odorata in the rat

Cited by 20 publications

References 9 publications

Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice

Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice

Composition and Antidiarrheal Activity ofBidens odorataCav.

Acetonic Fraction of Bidens pilosa Enriched for Maturase K Is Able to Control Cerebral Parasite Burden in Mice Experimentally Infected With Toxoplasma gondii

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