Diurnal Changes in Photosynthesis in Sugarcane Leaves: I. Carbon dioxide exchange rate, photosynthetic enzyme activities and metabolite levels relating to the C₄pathway and the Calvin cycle

Diurnal Changes in Photosynthesis in Sugarcane Leaves: II. Enzyme activities and metabolite levels relating to sucrose and starch metabolism

“…NiF4) seed cane sections were germinated in vermiculite at the end of April 1995 in a greenhouse for two weeks, and then the seedlings were transplanted to a field (Faculty of Agriculture, Saga University, Saga, Japan). Plants were cultivated and managed as described in the accompanying paper (Du et al, 2000).…”

“…Gas exchange was first measured with a Portable Photosynthesis System (LI-6200, Li-COR, Inc., USA), and then leaf sections were immediately immersed in liquid nitrogen. Before analysis, leaf samples were stored at -80°C as described in the component paper (Du et al, 2000).…”

“…The diurnal changes in CER and photosynthetically active radiation are cited from the component paper (Du et al, 2000) and provided here as a reference (Fig. lA).…”

Interplay of light and temperature during the in planta modulation of C4 phosphoenolpyruvate carboxylase from the leaves of Amaranthus hypochondriacus L.: diurnal and seasonal effects manifested at molecular levels

“…In leaves of Saccharum sp., PEPC activity reached a maximum at 14.00 h (Du et al , 2000). In maize, PEPC activity showed a diurnal rhythm when assayed at suboptimal pH and PEP concentrations (Kalt-Torres et al , 1987).…”

Relógio circadiano em eucariotos fotossintetizantes (Archaeplastida) e adaptação ao estresse

“…Este é o primeiro registro de oscilações infradianas (período de oscilação maior que 24h) de respostas fisiológicas para uma linhagem de cana-de-açúcar, além de detalhar como a fotossíntese dessa gramínea se comporta em condições de livre curso. Já se demonstrou que potencial hídrico das folhas, fotossíntese líquida, condutância estomática, taxa de expansão foliar, enzimas do metabolismo de carbono e acúmulo de sacarose e amido exibem oscilações em condições diurnas (DU et al, 2000a(DU et al, , 2000bROBERTS et al, 1990). Entretanto, estudos em condições circadianas (que necessitam de luz constante) são raramente explorados por agrônomos que, em geral, buscam mimetizar ao máximo as condições ambientais naturais.…”