Divalent ion encapsulated nano titania on Ti metal as a bioactive surface with enhanced protein adsorption

Anbazhagan, Esaitamil; Rajendran, A.; Duraipandy, Natarajan; Kiran, Manikantan Syamala; Pattanayak, Deepak K.

doi:10.1016/j.colsurfb.2016.03.009

Cited by 19 publications

(13 citation statements)

References 40 publications

(48 reference statements)

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“…Protein adsorption on the surface of all medical biomaterials occurs quickly during transplantation into human physiological media. 1 , 2 The distribution and conformational arrangement of protein nanobiofilms directly influence subsequent biological events, including cellular attachment, prefiltration and migration, 3 , 4 inflammatory responses, 5 and metal ion release (e.g., corrosion and biodegradation processes). 6 − 8 It is known that the adsorption of protein molecules on biomaterial surfaces is a complex process that involves different interactions at the protein molecule/solid interface, including hydrophobic, electrostatic, van der Waals, and hydrogen bonding forces.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

et al. 2022

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The formation of a protein nanobiofilm on the surface of degradable biomaterials such as magnesium (Mg) and its alloys influences metal ion release, cell adhesion/spreading, and biocompatibility. During the early stage of human body implantation, competition and interaction between inorganic species and protein molecules result in a complex film containing Mg oxide and a protein layer. This film affects the electrochemical properties of the metal surface, the protein conformational arrangement, and the electronic properties of the protein/Mg oxide interface. In this study, we discuss the impact of various simulated body fluids, including sodium chloride (NaCl), phosphate-buffered saline (PBS), and Hanks’ solutions on protein adsorption, electrochemical interactions, and electrical surface potential (ESP) distribution at the adsorbed protein/Mg oxide interface. After 10 min of immersion in NaCl, atomic force microscopy (AFM) and scanning Kelvin probe force microscopy (SKPFM) showed a higher surface roughness related to enhanced degradation and lower ESP distribution on a Mg-based alloy than those in other solutions. Furthermore, adding bovine serum albumin (BSA) to all solutions caused a decline in the total surface roughness and ESP magnitude on the Mg alloy surface, particularly in the NaCl electrolyte. Using SKPFM surface analysis, we detected a protein nanobiofilm (∼10–20 nm) with an aggregated and/or fibrillary morphology only on the Mg surface exposed in Hanks’ and PBS solutions; these surfaces had a lower ESP value than the oxide layer.

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Section: Introductionmentioning

confidence: 99%

Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

et al. 2022

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“…Hence, to accelerate the bioactivity of alkali and heat-treated Ti metal, the sodium ions need to be removed by a subsequent hot water treatment. This sodium-free alkali and heat-treated Ti metal has also been found to be bioactive as well as bond to living bone postimplantation using an animal model. , Rather than removing Na + ions by acid/water treatment, it can be replaced by more biocompatible elements like Ag + /Ca 2+ /Mg 2+ /Sr 2+ ions, which will not only remove cytotoxic Na + ions but also provide the Ti metal surface with additional properties. − …”

Section: Introductionmentioning

confidence: 99%

Effect of Silver-Containing Titania Layers for Bioactivity, Antibacterial Activity, and Osteogenic Differentiation of Human Mesenchymal Stem Cells on Ti Metal

Rajendran

Kapoor

Jothinarayanan

et al. 2019

ACS Appl. Bio Mater.

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Biomaterials with better osteogenic capacity, rapid osteo-integration, and higher mechanical strength are undoubtedly preferred for successful bone implant development. A porous sodium hydrogen titanate layer was formed on Ti metal by NaOH treatment, and the Na + ions were replaced by Ag + ions by subsequent AgNO 3 treatment that formed silver-containing hydrogen titanate. Heat treatment at 600 °C transformed sodium hydrogen titanate into sodium titanate with sheet-like morphology, whereas silver-containing hydrogen titanate was converted to anatase TiO 2 with an elongated rod-like structure. Further increment in temperature lead to the formation of rutile TiO 2 with distracted network morphology. Between these two, the anatase TiO 2 was ascertained to be bioactive by being capable of forming bonelike apatite in simulated body fluid within a period of 12 h. The concentration of silver on Ti metal was further optimized for better antibacterial activity against S. aureus and biocompatibility toward bone cells. A detailed investigation of thus optimized silver-containing Ti metal on the proliferation and differentiation of multipotent human mesenchymal stem cells further proved their biocompatibility nature and facilitation of osteogenic differentiation, thereby conferring those as ideally suited materials for bioimplant development in bone tissue engineering.

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“…Nevertheless, controlling the interactions and connecting to the bulk behavior remains a challenge. In that context, the use of multivalent ions [24][25][26] offers a viable path, with the unique opportunity to tailor and even invert the charge state of proteins as well as surfaces by overcompensation [16,17,27], which has been demonstrated to be a rather universal approach [28].…”

mentioning

confidence: 99%

Multivalent-Ion-Activated Protein Adsorption Reflecting Bulk Reentrant Behavior

Fries¹,

Stopper²,

Braun³

et al. 2017

Phys. Rev. Lett.

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Protein adsorption at the solid-liquid interface is an important phenomenon that often can be observed as a first step in biological processes. Despite its inherent importance, still relatively little is known about the underlying microscopic mechanisms. Here, using multivalent ions, we demonstrate the control of the interactions and the corresponding adsorption of net-negatively charged proteins (bovine serum albumin) at a solid-liquid interface. This is demonstrated by ellipsometry and corroborated by neutron reflectivity and quartz-crystal microbalance experiments. We show that the reentrant condensation observed within the rich bulk phase behavior of the system featuring a nonmonotonic dependence of the second virial coefficient on salt concentration c_{s} is reflected in an intriguing way in the protein adsorption d(c_{s}) at the interface. Our findings are successfully described and understood by a model of ion-activated patchy interactions within the framework of the classical density functional theory. In addition to the general challenge of connecting bulk and interface behavior, our work has implications for, inter alia, nucleation at interfaces.

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Divalent ion encapsulated nano titania on Ti metal as a bioactive surface with enhanced protein adsorption

Cited by 19 publications

References 40 publications

Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

Effect of Silver-Containing Titania Layers for Bioactivity, Antibacterial Activity, and Osteogenic Differentiation of Human Mesenchymal Stem Cells on Ti Metal

Multivalent-Ion-Activated Protein Adsorption Reflecting Bulk Reentrant Behavior

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