Divergent roles of the Drosophila melanogaster globins

Burmester, Thorsten; Wawrowski, Agnes; Diepenbruck, Ines; Schrick, Katharina; Seiwert, Nina; Ripp, Fabian; Prothmann, Andreas; Hankeln, Thomas

doi:10.1016/j.jinsphys.2017.06.003

Cited by 7 publications

(11 citation statements)

References 42 publications

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“…. ; apparently many-but probably not all-pancrustacean genomes or transcriptomes harbor an HbL [hemoglobin-like] gene", and the more recent hypothesis [27](pg. 230) that ".…”

Section: Plos Onementioning

confidence: 99%

“…To address the lack of hemoglobins in the remaining 89 transcriptomes, sample data submitted with each transcriptome's entry in NCBI was reviewed. Hemoglobin is not universally expressed across all tissue types and developmental stages, so targeted sampling used in many studies, such as specific tissues or developmental stages, could affect the presence or absence of hemoglobin transcripts (e.g., [16,27,51]). For example, hemoglobin expression in chironomids is known to be primarily in larval stages, and certain hemoglobins in Chironomus thummi are expressed during particular larval instars.…”

Section: Source Data-sampling Of Orders Rejected Transcriptomes Conmentioning

confidence: 99%

“…the evolution of metazoans [19]. Hemoglobin sequences have diverged significantly to accommodate various roles [1], with many other functions likely to be uncovered (e.g., [4], [27]). Indeed, compared to vertebrate hemoglobins, those of invertebrates show much greater sequence and structural variation [1], perhaps just a reflection of the enormous diversity of the latter in terms of species numbers, body plans, physiology, and life histories.…”

Section: Plos Onementioning

confidence: 99%

“…Extensive studies by Burmester and colleagues have revealed the surprising occurrence of hemoglobins in insects that seem to have little or no oxygen limitations, including the wellknown Apis mellifera, Drosophila melanogaster, Anopheles mosquitoes, and a dozen species of true bugs, beetles, and moths ( Table 1). Most of these characterized hemoglobins are implicated in respiration and associated with the tracheal system, though a few are involved in other physiological and developmental processes (e.g., [1,27,28]). The majority appear to be localized intracellularly, likely cytoplasmic or in the cell membranes of tracheocytes and adipocytes; a few are extracellular, dissolved in the hemolymph and are responsible for its red coloration.…”

Section: Introductionmentioning

confidence: 99%

“…The majority appear to be localized intracellularly, likely cytoplasmic or in the cell membranes of tracheocytes and adipocytes; a few are extracellular, dissolved in the hemolymph and are responsible for its red coloration. Although relatively few insect hemoglobins have been studied in detail, their copy number is variable and appear to show stage-and tissue-specific expression differences [27]. Substantial hemoglobin structural variation also occurs in insects, ranging from monomeric to di-, tetra-, and hexameric quaternary structures.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomes reveal expression of hemoglobins throughout insects and other Hexapoda

Herhold

Davis²,

Grimaldi³

2020

PLoS ONE

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Insects have long been thought to largely not require hemoglobins, with some notable exceptions like the red hemolymph of chironomid larvae. The tubular, branching network of tracheae in hexapods is traditionally considered sufficient for their respiration. Where hemoglobins do occur sporadically in plants and animals, they are believed to be either convergent, or because they are ancient in origin and their expression is lost in many clades. Our comprehensive analysis of 845 Hexapod transcriptomes, totaling over 38 Gbases, revealed the expression of hemoglobins in all 32 orders of hexapods, including the 29 recognized orders of insects. Discovery and identification of 1333 putative hemoglobins were achieved with target-gene BLAST searches of the NCBI TSA database, verifying functional residues, secondary-and tertiary-structure predictions, and localization predictions based on machine learning. While the majority of these hemoglobins are intracellular, extracellular ones were recovered in 38 species. Gene trees were constructed via multiple-sequence alignments and phylogenetic analyses. These indicate duplication events within insects and a monophyletic grouping of hemoglobins outside other globin clades, for which we propose the term insectahemoglobins. These hemoglobins are phylogenetically adjacent and appear structurally convergent with the clade of chordate myoglobins, cytoglobins, and hemoglobins. Their derivation and co-option from early neuroglobins may explain the widespread nature of hemoglobins in various kingdoms and phyla. These results will guide future work involving genome comparisons to transcriptome results, experimental investigations of gene expression, cell and tissue localization, and gas binding properties, all of which are needed to further illuminate the complex respiratory adaptations in insects.

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“…. ; apparently many-but probably not all-pancrustacean genomes or transcriptomes harbor an HbL [hemoglobin-like] gene", and the more recent hypothesis [27](pg. 230) that ".…”

Section: Plos Onementioning

confidence: 99%

Section: Source Data-sampling Of Orders Rejected Transcriptomes Conmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptomes reveal expression of hemoglobins throughout insects and other Hexapoda

Herhold

Davis²,

Grimaldi³

2020

PLoS ONE

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Potential benefits of Rehmanniae Radix after ancient rice‐steaming process in promotion of antioxidant activity in rats' health

Zhang

et al. 2023

Food Science & Nutrition

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Rice steam processed product of Rehmanniae Radix (RSRR), one of the processed products of Rehmanniae Radix (RR), is popular as an herbal medicine and food. However, the health‐promoting effects and mechanisms of RSRR are still unclear. In this study, 10‐week‐old Sprague–Dawley female rats were treated with different processed products of RR. No organ coefficient differences were observed between RSRR and the control group, indicating that RSRR did not cause damage to the rats. Compared with other RR products, superoxide dismutase, glutathione, and catalase levels were significantly higher and malondialdehyde levels were significantly lower in the RSRR group, indicating that RSRR exerted a better antioxidant effect. Gene expression analysis showed that hemoglobin genes (Hba‐a1, Hba‐a2, Hbb‐bs, Hbb, Hbq1b, Hbb‐b1, and LOC103694857) may be potential biomarkers to evaluate the antioxidant effect of RSRR. Antioxidation‐related signaling pathways in GO annotation, including cellular oxidant detoxification, hydrogen peroxide metabolic process, hemoglobin complex, and oxygen binding signaling pathways were significantly enriched, indicating these pathways may represent the antioxidant mechanism of RSRR. To explore the main active compounds primarily responsible for the antioxidant activity of RSRR, UPLC‐Q‐TOF‐MS was used and six components (catalpol, rehmannioside A, rehmannioside D, melittoside, ajugol, and verbascoside) were identified in rat serum. Catalpol and rehmannioside A were predicted to be the major active components by network pharmacology. These results suggested that RSRR exhibits antioxidant activity and has health‐promoting properties. This study provides a scientific basis for the antioxidant mechanism and clinical use of RSRR.

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Androglobin gene expression patterns and FOXJ1-dependent regulation indicate its functional association with ciliogenesis

Koay

Osterhof

Orlando

et al. 2021

Journal of Biological Chemistry

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Androglobin (ADGB) represents the latest addition to the globin superfamily in metazoans. The chimeric protein comprises a calpain domain and a unique circularly permutated globin domain. ADGB expression levels are most abundant in mammalian testis, but its cell-type-specific expression, regulation, and function have remained unexplored. Analyzing bulk and single-cell mRNA-Seq data from mammalian tissues, we found that—in addition to the testes—ADGB is prominently expressed in the female reproductive tract, lungs, and brain, specifically being associated with cell types forming motile cilia. Correlation analysis suggested coregulation of ADGB with FOXJ1, a crucial transcription factor of ciliogenesis. Investigating the transcriptional regulation of the ADGB gene, we characterized its promoter using epigenomic datasets, exogenous promoter-dependent luciferase assays, and CRISPR/dCas9-VPR-mediated activation approaches. Reporter gene assays revealed that FOXJ1 indeed substantially enhanced luciferase activity driven by the ADGB promoter. ChIP assays confirmed binding of FOXJ1 to the endogenous ADGB promoter region. We dissected the minimal sequence required for FOXJ1-dependent regulation and fine mapped the FOXJ1 binding site to two evolutionarily conserved regions within the ADGB promoter. FOXJ1 overexpression significantly increased endogenous ADGB mRNA levels in HEK293 and MCF-7 cells. Similar results were observed upon RFX2 overexpression, another key transcription factor in ciliogenesis. The complex transcriptional regulation of the ADGB locus was illustrated by identifying a distal enhancer, responsible for synergistic regulation by RFX2 and FOXJ1. Finally, cell culture studies indicated an ADGB-dependent increase in the number of ciliated cells upon overexpression of the full-length protein, confirming a ciliogenesis-associated role of ADGB in mammals.

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Divergent roles of the Drosophila melanogaster globins

Cited by 7 publications

References 42 publications

Transcriptomes reveal expression of hemoglobins throughout insects and other Hexapoda

Transcriptomes reveal expression of hemoglobins throughout insects and other Hexapoda

Potential benefits of Rehmanniae Radix after ancient rice‐steaming process in promotion of antioxidant activity in rats' health

Androglobin gene expression patterns and FOXJ1-dependent regulation indicate its functional association with ciliogenesis

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