Diverse Applications of DNAzymes in Computing and Nanotechnology

Towards the development of a DNA automaton: modular RNA-cleaving deoxyribozyme logic gates regulated by miRNAs

“…Concepts inspired by Boolean logic were applied for the realization of neuronal networks, 15 anticancer drug releasing containers, 16 synthetic multicellular biological computers 17 and logic circuits. 18 The current study is focusing on the development of modular DNA logic gates that employ miRNA markers as input signals and nucleic acid substrate cleavage as output signals for further implementation into bio-computation, diagnostic or therapeutic systems.…”

“…24 RNA-cleaving Dzs 25 are attractive candidates for the development of DNA logic gates that can solve the aforementioned issues due to their (1) catalytic activity, (2) ability of multiple turnover reactions which lead to significant signal amplification, and (3) flexible sequence adaptation to interact with virtually any input and/or target nucleic acid sequence. 14,18 In 2013, Kahan-Hanum M. et al utilized binary 10-23 Dz (biDz) to create DNA logic gates with potential clinical applications. 26 However, challenges in sensitivity, false-positive detection, and signal leakage prompted the need for optimization.…”