Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis

Rottmann, William H.; Meilan, Richard; Sheppard, Lorraine; Brunner, Amy M.; Skinner, Jeffrey S.; Ma, Cathleen; Cheng, Shuping; Jouanin, Lise; Pilate, Gilles; Strauss, Steven H.

doi:10.1046/j.1365-313x.2000.00734.x

Cited by 198 publications

(146 citation statements)

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“…4). Early onset of flowering in transgenic plants is in concordance with the earlier reports of Leafy overexpression in different species tobacco Nilsson 1995), aspen (Wiegel andNilsson 1995), rice (He et al 2000), poplar (Rottmann et al 2000), citrus (Pena et al 2001) and B. juncea (Roy et al 2009). The PCR positive lines also showed different morphological defects.…”

Section: Phenotypic Analysissupporting

confidence: 89%

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Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering

Sahni

Ganie

Narula

et al. 2013

Physiol Mol Biol Plants

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High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2 mg/l) + BA (3 mg/l) proved best for shoot regeneration with 89.9 % regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12 weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16 weeks. The maximum transformation frequency was 4 %.

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Section: Phenotypic Analysissupporting

confidence: 89%

“…Gray ex Hook.) (Rottmann et al 2000), and Citrus (Pena et al 2001). This ability of LFY to accelerate flowering is tapped in this study to develop early flowering transgenics of Brassica by overexpression of AtLFY.…”

Section: Introductionmentioning

confidence: 99%

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering

Sahni

Ganie

Narula

et al. 2013

Physiol Mol Biol Plants

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“…Regions of exon 3 in LFY also showed greater than expected latitudinal differentiation. Multiple functional studies have shown that LFY affects flowering and other developmental phenotypes, including in Populus (Rottman et al 2000). In contrast to the very high levels of segregating variation in Populus LFY, Arabidopsis LFY harbors low diversity consistent with a recent selective sweep .…”

Section: Molecular Adaptation Of Phenology Network Genesmentioning

confidence: 99%

Local Selection Across a Latitudinal Gradient Shapes Nucleotide Diversity in Balsam Poplar, Populus balsamifera L

et al. 2011

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Molecular studies of adaptive evolution often focus on detecting selective sweeps driven by positive selection on a specieswide scale; however, much adaptation is local, particularly of ecologically important traits. Here, we look for evidence of range-wide and local adaptation at candidate genes for adaptive phenology in balsam poplar, Populus balsamifera, a widespread forest tree whose range extends across environmental gradients of photoperiod and growing season length. We examined nucleotide diversity of 27 poplar homologs of the flowering-time network-a group of genes that control plant developmental phenology through interactions with environmental cues such as photoperiod and temperature. Only one gene, ZTL2, showed evidence of reduced diversity and an excess of fixed replacement sites, consistent with a species-wide selective sweep. Two other genes, LFY and FRI, harbored high levels of nucleotide diversity and exhibited elevated differentiation between northern and southern accessions, suggesting local adaptation along a latitudinal gradient. Interestingly, FRI has also been identified as a target of local selection between northern and southern accessions of Arabidopsis thaliana, indicating that this gene may be commonly involved in ecological adaptation in distantly related species. Our findings suggest an important role for local selection shaping molecular diversity and reveal limitations of inferring molecular adaptation from analyses designed only to detect species-wide selective sweeps.

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“…), as in other species, the rapid development of large leaves is an important determinant of productivity (Ridge et al, 1986; Barigah et al, 1994). In addition, Populus is very quickly becoming recognized as the "model" forest tree, equivalent to Arabidopsis (Taylor, 2002), because it is one of a few woody species where transformation is routine (Rottmann et al, 2000), where a large genomic initiative with several thousand expressed sequence tags is already developed (Sterky et al, 1998), and where the complete physical sequence of the genome will be completed within the next 2 years, as described by Wullschleger et al (2002).1 This work was supported by the EC through its Environment R and D program within the Fourth Framework as research contract ENV4 -CT97-0657 (POPFACE) coordinated by the University of Viterbo and by Natural Environment Research Council and Department of Environment, Food and Rural Affairs (grant nos. GR9/04077 and NFO410 to GT).…”

mentioning

confidence: 99%

Spatial and Temporal Effects of Free-Air CO2Enrichment (POPFACE) on Leaf Growth, Cell Expansion, and Cell Production in a Closed Canopy of Poplar

et al. 2003

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Leaf expansion in the fast-growing tree, Populus ϫ euramericana was stimulated by elevated [CO 2 ] in a closed-canopy forest plantation, exposed using a free air CO 2 enrichment technique enabling long-term experimentation in field conditions. The effects of elevated [CO 2 ] over time were characterized and related to the leaf plastochron index (LPI), and showed that leaf expansion was stimulated at very early (LPI, 0-3) and late (LPI, 6-8) stages in development. Early and late effects of elevated [CO 2 ] were largely the result of increased cell expansion and increased cell production, respectively. Spatial effects of elevated [CO 2 ] were also marked and increased final leaf size resulted from an effect on leaf area, but not leaf length, demonstrating changed leaf shape in response to [CO 2 ]. Leaves exhibited a basipetal gradient of leaf development, investigated by defining seven interveinal areas, with growth ceasing first at the leaf tip. Interestingly, and in contrast to other reports, no spatial differences in epidermal cell size were apparent across the lamina, whereas a clear basipetal gradient in cell production rate was found. These data suggest that the rate and timing of cell production was more important in determining leaf shape, given the constant cell size across the leaf lamina. The effect of elevated [CO 2 ] imposed on this developmental gradient suggested that leaf cell production continued longer in elevated [CO 2 ] and that basal increases in cell production rate were also more important than altered cell expansion for increased final leaf size and altered leaf shape in elevated [CO 2 ].Given the importance of forests for global bioproductivity, the consequences of increased atmospheric [CO 2 ] for the global carbon cycle are potentially extremely large (Malhi et al., 1999). Despite this, there are still relatively few large-scale, long-term experiments from which predictions about likely forest responses can be made. Few studies have been completed where trees are allowed to develop to canopy closure and where a "stable" response to [CO 2 ] is likely. Determining the response of leaf area development to elevated [CO 2 ] is important. It is still unknown whether forests of the future will maintain a higher leaf area index (LAI), as implied from smalltree studies (Ceulemans et al., 1997) or whether the long-term (decades) responses will be reduced allocation to foliage and lower LAI, as suggested by some modeling approaches (Medlyn and Dewar, 1996) or involve acclimation to limited nitrogen (Oren et al., 2001).Leaf growth is often stimulated in short-term response to elevated [CO 2 ] (Taylor et al., 1994;Pritchard et al., 1999), and both leaf cell expansion and cell production are sensitive to [CO 2 ] (Taylor et al., 1994). It is likely that these processes respond to additional carbohydrate from photosynthesis and, as such, altered atmospheric [CO 2 ] provides a critical insight into how carbon regulates plant development and growth (Masle, 2000). The importance of leaf develo...

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Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis

Cited by 198 publications

References 32 publications

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering

Local Selection Across a Latitudinal Gradient Shapes Nucleotide Diversity in Balsam Poplar, Populus balsamifera L

Spatial and Temporal Effects of Free-Air CO2Enrichment (POPFACE) on Leaf Growth, Cell Expansion, and Cell Production in a Closed Canopy of Poplar

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