Diverse preparation methods for small intestinal submucosa (SIS): Decellularization, components, and structure

Ji, Yanhui; Zhou, Jinge; Sun, Tingfang; Tang, Kai; Xiong, Zekang; Ren, Zhengwei; Yao, Sheng; Chen, Kaifang; Yang, Fan; Zhu, Fengzhao; Guo, Xiaodong

doi:10.1002/jbm.a.36582

Cited by 58 publications

(52 citation statements)

References 42 publications

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“…The natural biomaterials have good biochemical activity and biological compatibility. However, in the process of handling natural materials, such as the decellularized tissues, the normal 3D structure is changed or some biological factors are lost [ 7 ]. Furthermore, the requirements of material characteristics vary with different kinds of tissue construction.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of small intestine submucosa in tissue engineering

Pan

Qin

et al. 2020

Regenerative Biomaterials

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With the development of tissue engineering, the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo. Especially, surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials. The small intestine submucosa (SIS) is an extracellular matrix isolated from the submucosal layer of porcine jejunum, which has good tissue mechanical properties and regenerative activity, and is suitable for cell adhesion, proliferation and differentiation. In recent years, SIS is widely used in different areas of tissue reconstruction, such as blood vessels, bone, cartilage, bladder and ureter, etc. This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds, including functional group bonding, protein adsorption, mineral coating, topography and formatting modification and drug combination. In addition, the reasonable combination of these methods also offers great improvement on SIS surface modification. This article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Surface modification of small intestine submucosa in tissue engineering

Pan

Qin

et al. 2020

Regenerative Biomaterials

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“…And bioabsorbable collagen membrane is the most frequently used barrier membrane in GBR treatments [6,7]. e extracellular matrix (ECM), which retains collagen and various signaling molecules containing glycosaminoglycans, glycoproteins, and abundant growth factors [8], has been used in the GBR technique for the purpose of building a tissue engineering scaffold [9][10][11][12]. Porcine-derived small intestinal submucosa (SIS) is an acellular, naturally derived ECM material from the submucosal layer of porcine intestinal tissue [13,14].…”

Section: Introductionmentioning

confidence: 99%

Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

Wang

Liu

et al. 2019

BioMed Research International

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Objective. The aim of the study is to evaluate the effects of multilaminated small intestinal submucosa (mSIS) combined with bone substitute material to repair peri-implant defects during guided bone regeneration procedures. Methods. Twelve implants were placed in bilateral lower premolars of three beagle dogs, and a peri-implant buccal bone defect (3 mm width and 4 mm height) was created at each implant site. A total of 12 sites were filled with a particulate bone substitute material and then randomly divided into three treatment groups: covered by mSIS membrane (mSIS group), covered by collagen membrane (BG group), and no treatment (control group), each group of four sites. After 12 weeks of healing, all of the animals were euthanized and dissected blocks were obtained for micro-computed tomography (micro-CT) and histological analyses. Results. Micro-CT results revealed similar horizontal width of augmented tissue and new bone formation between mSIS and BG groups (P<0.05). Histological analyses revealed that the differences in horizontal widths of newly formed bone and bone-to-implant contact between mSIS and BG groups were not significant (P>0.05). All of these parameters were significantly different from those in the control group (P<0.05). Conclusions. These findings confirmed that mSIS combined with the bone substitute material enhanced bone regeneration in peri-implant defects, in a manner similar to that of a collagen membrane.

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“…Although still undocumented as an efficient decellularized agent, trypsin can be combined with physical, acid-, base-, or detergent-based protocols [80][81][82]. The action of trypsin is achieved by immersion of the tissue in an isotonic solution containing from 0.25% to 0.1% w/v of enzyme, separately from the other treatments of the protocol.…”

Section: Enzymesmentioning

confidence: 99%

Decellularized Scaffolds for Skin Repair and Regeneration

Dussoyer

Michopoulou²,

Rousselle

2020

Applied Sciences

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The skin is the largest organ in the body, fulfilling a variety of functions and acting as a barrier for internal organs against external insults. As for extensive or irreversible damage, skin autografts are often considered the gold standard, however inherent limitations highlight the need for alternative strategies. Engineering of human-compatible tissues is an interdisciplinary and active field of research, leading to the production of scaffolds and skin substitutes to guide repair and regeneration. However, faithful reproduction of extracellular matrix (ECM) architecture and bioactive content capable of cell-instructive and cell-responsive properties remains challenging. ECM is a heterogeneous, connective network composed of collagens, glycoproteins, proteoglycans, and small molecules. It is highly coordinated to provide the physical scaffolding, mechanical stability, and biochemical cues necessary for tissue morphogenesis and homeostasis. Decellularization processes have made it possible to isolate the ECM in its native and three-dimensional form from a cell-populated tissue for use in skin regeneration. In this review, we present recent knowledge about these decellularized biomaterials with the potential to be used as dermal or skin substitutes in clinical applications. We detail tissue sources and clinical indications with success rates and report the most effective decellularization methods compatible with clinical use.

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Diverse preparation methods for small intestinal submucosa (SIS): Decellularization, components, and structure

Cited by 58 publications

References 42 publications

Surface modification of small intestine submucosa in tissue engineering

Surface modification of small intestine submucosa in tissue engineering

Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

Decellularized Scaffolds for Skin Repair and Regeneration

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