Diverse Responses of Lettuce Cultivars and Germplasm Lines to Infections of Three Isolates of Xanthomonas campestris pv. vitians

Wang, Yunwen; Lu, Huangjun; Raid, Richard N.; Nuessly, Gregg S.; Faroutine, Georgy

doi:10.21273/hortsci.50.5.650

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…The aggressiveness diversity of X. campestris pv. vitians strains and variation of host responses among lettuce genotypes are very important epidemiological aspects for understanding and controlling bacterial leaf spot of lettuce outbreaks (WANG et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Xanthomonas campestris pv. vitians is a potentially destructive pathogen, favored by high humid conditions, and is capable of spreading rapidly in a greenhouse through irrigation water (WANG et al, 2015), especially in greenhouses (TEBALDI et al, 2015). Typical symptoms of bacterial leaf spot include water soaked, translucent, and angular brown lesions, which turn black and eventually thin and dry out.…”

Section: Introductionmentioning

confidence: 99%

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Reaction of biofortified lettuce genotypes to different strains of Xanthomonas campestris pv. vitians

et al. 2022

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Lettuce bacterial leaf spot caused by Xanthomonas campestris pv. vitians is an aggressive disease that is difficult to control. So far there are no reports of the reaction of biofortified lettuce genotypes to different isolates of the bacteria. Thus, the objective was to evaluate the aggressiveness of X. campestris pv. vitians, as well as the reaction of biofortified lettuce genotypes to bacterial spot. Two experiments were performed in two distinct seasons (winter and summer), in greenhouse at the Vegetable Experimental Station of the Federal University of Uberlândia (UFU). The experimental design in both experiments was a randomized block design, in a factor scheme of 5 × 4 (five genotypes and four strains), with four repetitions. Were evaluated the severity and the area under the disease progress curve. In general, the biofortified lettuce 'Uberlândia 10000' was more resistant to most bacterial strains in the summer cultivation, and in the winter period UFU 'Crespa 206'. The commercial cultivar Robusta was the most susceptible to the strains during both seasons. The UFU E125 strain was the most aggressive for most genotypes in both seasons.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reaction of biofortified lettuce genotypes to different strains of Xanthomonas campestris pv. vitians

et al. 2022

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“…It has been previously demonstrated [46] that resistance to bacterial leaf spot in lettuce cultivars is conferred by the resistance gene Xar1. Bull et al [32] and Wan et al [47] observed moderate to high resistance to bacterial leaf spot when the cultivar Little Gem was artificially inoculated with X. hortorum pv. vitians isolates collected in California and Florida, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genotypic and Phenotypic Characterization of Lettuce Bacterial Pathogen Xanthomonas hortorum pv. vitians Populations Collected in Quebec, Canada

Hébert

Laforest

et al. 2021

Agronomy

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Bacterial leaf spot of lettuce, caused by Xanthomonas hortorum pv. vitians, is an economically important disease worldwide. For instance, it caused around 4 million CAD in losses in only a few months during the winter of 1992 in Florida. Because only one pesticide is registered to control this disease in Canada, the development of lettuce cultivars tolerant to bacterial leaf spot remains the most promising approach to reduce the incidence and severity of the disease in lettuce fields. The lack of information about the genetic diversity of the pathogen, however, impairs breeding programs, especially when disease resistance is tested on newly developed lettuce germplasm lines. To evaluate the diversity of X. hortorum pv. vitians, a multilocus sequence analysis was performed on 694 isolates collected in Eastern Canada through the summers of 2014 to 2017 and two isolates in 1996 and 2007. All isolates tested were clustered into five phylogroups. Six pathotypes were identified following pathogenicity tests conducted in greenhouses, but when phylogroups were compared with pathotypes, no correlation could be drawn. However, in vitro production of xanthan and xanthomonadins was investigated, and isolates with higher production of xanthomonadins were generally causing less severe symptoms on the tolerant cultivar Little Gem. Whole-genome sequencing was undertaken for 95 isolates belonging to the pathotypes identified, and de novo assembly made with reads unmapped to the reference strain’s genome sequence resulted in 694 contigs ranging from 128 to 120,795 bp. Variant calling was performed prior to genome-wide association studies computed with single-nucleotide polymorphisms (SNPs), copy-number variants and gaps. Polymorphisms with significant p-values were only found on the cultivar Little Gem. Our results allowed molecular identification of isolates likely to cause bacterial leaf spot of lettuce, using two SNPs identified through genome-wide association study.

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“…vitians ( Xcv ) is economically important worldwide (Lu and Raid, 2013). BLS infection of lettuce is favored by warm and humid climatic conditions (Wang et al, 2015). Typical symptoms are black, water-soaked lesions on the leaves (Hayes et al, 2014), which may coalesce under conditions favorable to Xcv development, causing large necrotic spots on the leaves (Toussaint, 1999).…”

Section: Introductionmentioning

confidence: 99%

The Ionomics of Lettuce Infected by Xanthomonas campestris pv. vitians

et al. 2019

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Bacterial leaf spot (BLS) caused by Xanthomonas campestris pv. vitians ( Xcv ) places a major constraint on lettuce production worldwide. The most sustainable strategy known to date for controlling BLS is the use of resistant cultivars. The nutrient elemental signature (ionome) of ten lettuce cultivars with three levels of resistance was analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) to determine which nutrient balances are linked to resistance to BLS, and to assess the effect of Xcv infection on the ionome. The elemental concentrations were preprocessed with isometric log-ratios to define nutrient balances. Using this approach, 4 out of 11 univariate nutrient balances were found to significantly influence the resistance of lettuce cultivars to BLS ( P < 0.05). These significant balances were the overall nutritional status balancing all measured nutrients with their complementary in the dry mass, as well as balances [Mn | Zn,Cu], [Zn | Cu], and [S,N | P]. Moreover, the infection of lettuce cultivars mostly affected the lettuce ionome on the [N,S | P] balance, where infection tended to lean the balance toward the N,S part relatively to P. This study shows that nutrient uptake in lettuce can be affected by BLS infection and that nutrient status influences resistance to BLS infection.

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Diverse Responses of Lettuce Cultivars and Germplasm Lines to Infections of Three Isolates of Xanthomonas campestris pv. vitians

Cited by 10 publications

References 18 publications

Reaction of biofortified lettuce genotypes to different strains of Xanthomonas campestris pv. vitians

Reaction of biofortified lettuce genotypes to different strains of Xanthomonas campestris pv. vitians

Genotypic and Phenotypic Characterization of Lettuce Bacterial Pathogen Xanthomonas hortorum pv. vitians Populations Collected in Quebec, Canada

The Ionomics of Lettuce Infected by Xanthomonas campestris pv. vitians

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