Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics

Widiastuti, Alfin; SOBIR, SOBIR; Suhartanto, M. Rahmad

doi:10.13057/nusbiosci/n020104

Cited by 10 publications

(8 citation statements)

References 5 publications

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“…It is explain the rate of photosynthesis is more efficient. Besides, the higher density of stomatal also allows the higher gas exchange, consequently, the rate of photosynthesis is higher than control [43]. The higher photosynthesis supports the plant growth.…”

Section: Results and Discusionsmentioning

confidence: 99%

Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

Kusmiyati

Sutarno

Sas

et al. 2018

IOP Conf. Ser.: Earth Environ. Sci.

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Abstract. Narrow genetic diversity is a main problem restricting the progress of soybean breeding. One way to improve genetic diversity of plant is through mutation. The purpose of this study was to investigate effect of different dose of gamma rays as induced mutagen on physiological, morphological, and anatomical markers during seed germination and seedling growth of soybean. Seeds of soybean cultivars Dering-1 were irradiated with 11 doses of gamma rays (0, 5, 10, 20, 40, 80, 160, 320, 640, 1280, and 2560 Gy [Gray]. The research design was arranged in a completely randomized block design in three replicates. Results showed that soybean seed exposed at high doses (640, 1280, and 2560 Gy) did not survive more than 20 days, the doses were then removed from anatomical evaluation. Higher doses of gamma rays siginificantly reduced germination percentage at the first count and final count, coefficient of germination velocity, germination rate index, germination index, seedling height and seedling root length, and significantly increased mean germination time, first day of germination, last day of germination, and time spread of germination. However, the effects of gamma rays were varies for density, width, and length of stomata. The LD 50 obtained based on survival percentage was 314.78 Gy. It can be concluded that very low and low doses of gamma rays (5-320 Gy) might be used to study the improvement of soybean diversity.

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Section: Results and Discusionsmentioning

confidence: 99%

Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

Kusmiyati

Sutarno

Sas

et al. 2018

IOP Conf. Ser.: Earth Environ. Sci.

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“…Mutations in the structure of chromosomes occur when a chromosome segment is reduced (deletion), translocation, duplication, or inversion. These changes in chromosomes can ultimately affect plant growth [16]. Genetic changes resulting from gamma-ray radiation occur due to the increase or increase of one or more bases contained in one DNA molecule [17].…”

Section: Plant Drymentioning

confidence: 99%

The Yield of M7 Mentik Wangi Rice Irradiated With Several Doses of Gamma Rays Under Drought Stress

Puspitasari

Kurniasih

Indradewa

2022

IOP Conf. Ser.: Earth Environ. Sci.

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The possible risk of yield losses due to drought stress could threaten rice production and national food security. One way to deal with drought limitations is by developing drought- tolerant varieties using gamma-ray irradiation. This research aimed to get the optimal dose of gamma-ray radiation to create an M7 generation of Mentik Wangi variety that is drought tolerant. Field experiments were conducted from December 2020 to March 2021 at the Screen House of the Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, the Laboratory of Soil Physics, and the Laboratory of Plant Production Management, Faculty of Agriculture, Universitas Gadjah Mada. This study has carried out using a Randomized Complete Block Design with two factors. The first factor is gamma-ray irradiation dose (0gy. 100gy, 200gy, 300gy), and the second factor is drought stress treatment (without drought stress and drought stress in the flowering stage) with three replications. The results showed no interaction between gamma-ray irradiation dose and drought stress on all observed variables except plant dry matter. Drought stress in the flowering stage increased the percentage of unfilled grain, reducing the harvest index and grain weight significantly by 40.9%. Gamma-ray irradiation in a range of 100 to 300 gy did not affect panicle numbers and length, also the percentage of unfilled grain, but reduced the number of grains per panicle, thereby reducing the harvest index and grain weight 45.0% compared to those without irradiation.

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“…Warna petiol pada tanaman yang diradiasi bervariasi mulai dari hijau kemerahan, hijau dengan kemerahan dekat batang atau daun yang berbeda dengan tanaman kontrolnya yaitu merah sepanjang tangkai daun (Gambar 1B). Respon tanaman terhadap perlakuan iradiasi sinar gamma bersifat individual, namun secara gambaran umum pada beberapa parameter menunjukkan terjadinya perubahan (Widiastuti et al., 2010). Keterangan: pengamatan karakter morfologi tanaman ini dilakukan pada lima asesi Ubi Kuning hasil radiasi sebelum diperbanyak steknya menjadi beberapa klon sampai tiga generasi Selain itu, karakter morfologi umbi, Ubi Kuning hasil radiasi terlihat berbeda dengan kontrol terutama dari intensitas warna umbinya (Tabel 2 dan Gambar 1).…”

Section: Larutan Standar Beta Karotenunclassified

Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro

et al. 2020

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In line with the increase in cassava production and the development of nutrient-rich cassava in order to support national food diversification and biofortification programs, the selection of selected varieties of cassava varieties, which are superior in nutrients especially rich in beta carotene is very necessary. Beta carotene is an important source of antioxidants to scavange free radicals and is a provitamin A precusor to form vitamin A. The development of superior cassava riching in beta carotene can be done through the mutation approach with gamma irradiation. The observation of changes in morphological characters and levels of beta carotene from irradiated cassava need to be done to get the superior beta carotene cassava clone candidates, which could be developed in the future. This experiment was conducted at the Biotechnology Research Center, LIPI. The sample used in this study originated from in vitro shoots from several Ubi Kuning clones resulting from 10 Gy radiation, which were then transferred to the field. Observations of morphological characters and levels of beta carotene of Ubi Kuning were carried out in the third generation, which was harvested at the age of 10 months. The morphological analysis of irradiated Ubi Kuning showed that there was a difference in the intensity of tuber color between some of irradiated Ubi Kuning clones compared to the control. The determination of beta carotene levels based on the standard beta carotene curve found that the highest content of beta carotene was found in the UK Rad 3.4 clone with beta carotene content of 0.252 μg / mL compared to the control (0.219 μg / mL). The lowest beta carotene content was obtained in UK Rad 3.3 (0.048 μg / mL), followed by UK Rad 3.2 (0.221 μg / mL) and UK Rad 4.1 (0.120 μg / mL). This shows that the irradiated Ubi Kuning at dosage of 10 Gy caused variations in the intensity of tuber colors and the content of beta carotene from the cassava.Keywords: Ubi Kuning, beta carotene, Gamma light irradiation

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Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics

Cited by 10 publications

References 5 publications

Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

The Yield of M7 Mentik Wangi Rice Irradiated With Several Doses of Gamma Rays Under Drought Stress

Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro

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