Diversity and Antimicrobial and Plant-Growth-Promoting Activities of Endophytic Fungi in Dendrobium loddigesii Rolfe

Chen, Xiao Mei; Dong, Huawei; Hu, Ke Xing; Sun, Zhi Rong; Chen, Juan; Guo, Shun

doi:10.1007/s00344-010-9139-y

Cited by 76 publications

(52 citation statements)

References 29 publications

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“…Endophytic fungi, as mutualistic plant symbionts, Table S1). The present study corroborates previous works in the same field showing that there exists a rich endophyte assemblage in Dendrobium 14,[23][24][25][26][27] . The data revealed that the endophytic fungi population of D. officinale harboured a group of cosmopolitan species such as F. oxysporum, X. cubensis, Colletotrichum sp., Alternaria sp.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Cultural Endophytic Fungi Associated with <i>Dendrobium Officinale</i>:Identification, Diversity Estimation and their Antimicrobial Potential

Jin¹,

Li²,

Liu³

et al. 2017

Current Science

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Endophytic fungi associated with Chinese medicinal plant Dendrobium officinale have been revealed using the culture-dependent approach and the diversity and antimicrobial activity have been examined in this study. A total of 105 endophytic isolates representing 16 fungal morphotypes were recovered from the roots, stems and leaves of D. officinale. Identification by ITS-rDNA sequencing showed 12 distinct operational taxonomic units, and most of them were classified into species level. Shannon's and Simpson's indices were calculated to quantify fungal diversity. The results demonstrated that endophytic fungi associated with D. officinale were moderately diverse, and fungal species among three tissues was also different which exhibited tissue-specificity. The composition of endophyte assemblages from the roots was most abundant and diverse, followed by the stems, while the leaves possessed relatively low isolation rate. Fusarium oxysporum (21.0%) and Xylaria cubensis (20.0%) were the most dominant species, while Colletotrichum sp. and Pestalotiopsis sp. were also frequently isolated. Three species (F. oxysporum, Aspergillus niger and Aureobasidium pullulan) showed broad-spectrum antimicrobial activity, and F. oxysporum had acute inhibition to pathogens tested. Our systematic study could enrich the current knowledge on diversity of endophytic fungi. The fungal isolates from D. officinale may be used for exploration of novel bioactive compounds and have potential as biocontrol agents.

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Section: Discussionsupporting

confidence: 92%

“…However, high frequency of Xylaria sp. was not reported in D. loddigesii in a previous study 26 . Recent relevant literature on Dendrobium endophytes support the idea that fungal endophytes might exhibit tissue specificity.…”

Section: Discussionmentioning

confidence: 51%

Cultural Endophytic Fungi Associated with <i>Dendrobium Officinale</i>:Identification, Diversity Estimation and their Antimicrobial Potential

Jin¹,

Li²,

Liu³

et al. 2017

Current Science

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“…Of all the Ascomycetes obtained, 71% of the fungal endophytes belonged to the genus Fusarium. These results correlate with those obtained from a study by Bai et al (2009), Chen et al (2010 and Xing et al (2011) which demonstrated that the dominant fungal endophyte strains isolated so far belong to the genus Fusarium. Although, Fusarium spp.…”

Section: Isolation and Identification Of The Fungal Endophytessupporting

confidence: 91%

“…Fungal endophyte diversity in plants could be affected by environmental factors, host species as well as the host genotypes (Chen et al, 2010). Various research works show that endophytic fungi mostly consist of members of Ascomycota although some taxa of Basidiomycota, Zygomycota and Oomycote have also been reported (Guo et al, 2001).…”

Section: Isolation and Identification Of The Fungal Endophytesmentioning

confidence: 99%

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

Wanga¹,

Wagara²,

Mwakubambanya³

et al. 2018

Afr. J. Biotechnol.

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Antibacterial activity of extracts of Zanthoxylum gilletii, Markhamia lutea and their fungal endophytes were evaluated against bacterial pathogens of common bean: Xanthomonus axonopodis pv. phaseoli and Pseudomonus syringae pv. phaseolicola. The leaves of both plants were dried under shade, ground to fine powder and extracted using methanol. The methanol extracts were fractionated sequentially using ethylacetate and hexane to produce various fractions. Endophytic fungi were isolated from fresh leaves and identified by ITS-rDNA sequence analysis. Antibacterial screening of the fungal endophytes was done by dual culture assay. The most active endophytic fungi were fermented on rice media and extracted using methanol. Pure compounds were analyzed by a combination of mass spectrometry and spectroscopic techniques which included 1D and 2D NMR. Antibacterial activity of all the extracts was determined by disc agar diffusion assay against the test organisms. Twenty-four (24) fungal endophytes were isolated which included: Fusarium, Chaetomium, Scopulariopsis and Trametes. Endophytic Fusarium solani was the most active against X. axonopodis pv. phaseoli (20.3 mm inhibition zone) and P. syringae pv. phaseolicola (18.6 mm inhibition zone). The plant extracts were active against X. axonopodis pv. phaseoli with an inhibition zone ranging between 8-12 mm except the methanol extract from Z. gilletii which did not show any activity. The endophytic extracts were active against both test organisms with a zone of inhibition ranging from 9.3-14 mm. Phenolic compounds present in Fusarium species may have contributed to the antibacterial activity of this strain against the test organisms.

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“…Alguns autores têm sugerido que os DSEF sejam capazes de estabelecer associações semelhantes aos fungos micorrízicos; atuando como promotores do crescimento vegetal, principalmente por facilitarem a absorção de nutrientes como o fósforo e nitrogênio (SCERVINO et al, 2009;CHEN et al, 2010), inclusive com a produção de enzimas extracelulares capazes de hidrolisar compostos complexos de carbono, nitrogênio e fósforo (MANDYAM;JUMPPONEN, 2005). Além disso, tem sido sugerido que a melanização da parede celular fúngica e, a consequente produção de hifas melanizadas, representa fator que contribui para a adaptação e sobrevivência do hospedeiro a condições adversas, uma vez que a melanina teria um papel importante na eliminação de radicais de oxigênio gerados durante o estresse abiótico (REDMAN et al, 2002).…”

Section: Introductionunclassified

Isolamento, armazenamento e determinação da colonização por fungos “dark septate” a partir de plantas de arroz

Ribeiro¹,

Pereira²,

Mosqueira³

et al. 2011

AGRO@MBIENTE

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Resumo -Os fungos endofíticos conhecidos como DSEF ou "dark septate" podem ser promotores de crescimento vegetal. Com o objetivo de aperfeiçoar uma metodologia para o isolamento, armazenamento e determinação da colonização de raízes de arroz por fungos "dark septate" desenvolveu-se um estudo a partir da obtenção destes microrganismos em plantas de Oryza glumaepatula coletadas em ambientes amazônicos. As raízes das plantas de doze amostras de O. glumaepatula foram seccionadas, desinfestadas e dispostas em placas com o meio ágar-malte adicionado de cloranfenicol, sulfametazol e trimetropina. Os isolados obtidos foram avaliados quanto às características de "dark septate" e armazenados em água destilada com e sem a adição de glicerol. Cinco isolados fúngicos considerados "dark septate" foram reinoculados em plantas de O. glumaepatula e em O. sativa e, também, caracterizados molecularmente através da amplificação e sequenciamento da região ITS1-5.8S-ITS2. O meio de cultura ágar-malte foi eficiente para a obtenção de "dark septate", obtendo-se mais de 50 isolados. O armazenamento em água destilada e em água destilada adicionada de glicerol mostrou-se eficiente para a manutenção das culturas fúngicas por pelo menos 12 meses. Os cinco isolados fúngicos reinoculados foram capazes de colonizar tanto raízes O. glumaepatula quanto O. sativa, não desencadeando sintomas de patogenicidade. A análise molecular mostrou que estes fungos apresentam baixa similaridade entre eles e não possuem similaridade com fungos "dark septate" já descritos, indicando que a metodologia empregada permitiu a obtenção de espécies fúngicas diversas e que a espécie O. glumaepatula abriga alta diversidade deste grupo de fungos. Palavras-Chave -Amazônia. DSEF. Oryza glumaepatula. Oryza sativa.Abstract -Endophyte fungi known as DSEF can be plant-growth-promoting. Aiming to improve a methodology for the isolation, storage and determine the rice roots colonization by "dark septate", a study was developed based on the achievement of these microorganisms in Oryza glumaepatula plants collected in Amazon region. The plant roots of twelve O. glumaepatula samples were sectioned, disinfected, and placed in plates with agar-malte medium added of chloramphenicol, sulphametazole and trimetropin. The isolates were evaluated in relation of dark septate characteristics and stored in distilled water, and in distilled water added of glycerol. Five isolates considered dark septate were re-inoculated in O. glumaepatula and O. sativa, and also analyzed through amplification and sequencing of ITS1-5.8S-ITS2 region. The agar-malte medium was efficient to isolate dark septate, and more than 50 isolates were obtained. The distilled water and also distilled water added of glycerol were efficient to maintain the fungus culture at least 12 months. The five isolates re-inoculated were able to colonize both O. glumaepatula and O. sativa, without pathogenic symptoms observed. The molecular approach reveled that these fungus are not similar to each other and also to others dark septa...

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Diversity and Antimicrobial and Plant-Growth-Promoting Activities of Endophytic Fungi in Dendrobium loddigesii Rolfe

Cited by 76 publications

References 29 publications

Cultural Endophytic Fungi Associated with <i>Dendrobium Officinale</i>:Identification, Diversity Estimation and their Antimicrobial Potential

Cultural Endophytic Fungi Associated with <i>Dendrobium Officinale</i>:Identification, Diversity Estimation and their Antimicrobial Potential

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

Isolamento, armazenamento e determinação da colonização por fungos “dark septate” a partir de plantas de arroz

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