Diversity and bioprospecting of extremely halophilic archaea isolated from Algerian arid and semi-arid wetland ecosystems for halophilic-active hydrolytic enzymes

Menasria, Taha; Aguilera, Margarita; Hacène, Hocine; Benammar, Leyla; Ayachi, Ammar; Bachir, Abdelkrim Si; Dekak, Ahmed; Monteoliva‐Sánchez, Mercedes

doi:10.1016/j.micres.2017.12.011

Cited by 65 publications

(51 citation statements)

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“…For their biotechnological applications, extreme and moderate halophilic bacteria have received high attention (Edbeib et al, 2016;Margesin and Schinner 2001;Oren, 2002). However, culture-based methods remain an essential approach to obtaining new microbes that offer application of new potentials and an understanding of their ecophysiological and environmental functions (Menasria et al, 2018). Throughout this work, we have characterized seven moderate halophiles and one extreme halophilic isolate.…”

Section: Isolation and Phylogenetic Characterization Of The Halophilimentioning

confidence: 99%

“…The complete 16S rRNA gene sequence of the strain ATS1 was 1490 bp (Genbank accession number: MF353936) and showed 98% significant sequence similarity to that of the Haloferax mediterranei strain ATCC 33500T. This species was found in Sebkha Ezzemoul (Ain M'Lila) (Kharroub, 2007), Sidi Ameur (Bousaâda) (Quadri et al, 2016) as well as in Sebkha Medghacen and Sebkha El Hamiet (Menasria et al, 2018).…”

Section: Isolation and Phylogenetic Characterization Of The Halophilimentioning

confidence: 99%

“…Menasria et al (2019) report that the enzymes amylase, esterase, gelatinase and nuclease activities are classified first in the group of hydrolases. While Menasria et al (2018), report that the esterase and inulinase activities have the highest activities. Probably because biotopes represent an important reservoir of organic matter which can stimulate the metabolic activity of isolated halophilic archaea.…”

Section: Screening For Halophilic-active Hydrolytic Enzymesmentioning

confidence: 99%

“…In addition, Algeria has several wetlands and hypersaline lakes, with a specific typology and ecology, of which 50 are classified as sites of international importance as Ramsar sites (Aliat et al, 2016). However, all aspects related to the microbiota composition (diversity and bioactivity) are few studied and remain unidentified (Hacene et al, 2004;Kebbouche-Gana et al, 2009;Boutaiba et al, 2011;Kharroub et al, 2014;Quadri et al, 2016;Menasria et al, 2018;.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Akmoussi-Toumi¹,

Khemili²,

Kebbouche-Gana³

et al. 2019

Current Research in Bioinformatics

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At the limits of life, hyper-saline aquatic ecosystems; Chott and Sebkha are a model of choice of extreme environments, housing a halophilic microflora that had to adapt to these conditions. In Algeria, these ecosystems are poorly studied. However, our study was carried out on the waters of Chott Tinsilt and Sebkha El Malah. The study of this microflora revealed the presence of a significant morphological, physiological and metabolic diversity. The molecular study allowed us to access to a phylogenetic affiliation including an Archean Species (ATS1) and 7 bacterial species (A1, A2, A3, A4, B1, B4, B5). The results showed that these isolates were related to the genera Haloferax (for the strain ATS1) and Halomonas (strains A1, A2 and A4), Staphylococcus (strain A3), Salinivibrio (strain B1), Planococcus (strain B4) and Halobacillus (strain B5). Most isolates produced hydrolases at high salt concentrations. The Production yields obtained are very promising for applications in the biotechnology and industrial microbiology.

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Section: Isolation and Phylogenetic Characterization Of The Halophilimentioning

confidence: 99%

Section: Isolation and Phylogenetic Characterization Of The Halophilimentioning

confidence: 99%

Section: Screening For Halophilic-active Hydrolytic Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Akmoussi-Toumi¹,

Khemili²,

Kebbouche-Gana³

et al. 2019

Current Research in Bioinformatics

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“…Located in the eco-complex wetland of the high plains (Hauts Plateaux) of northeastern Algeria, Chott Tinsilt is a temporary salt lake, classified as a Ramsar site since 2004 (DGF, 2004). For this site, apart from some works on vegetation (Bezzalla et al, 2019a) and microorganisms (Menasria et al, 2018), most of the studies have targeted the avifauna (Boulkhssaim et al, 2016;Bezzalla et al, 2019b). The entomofauna has been little investigated, except for the studies by Zerguine (2014), on Chironomidae (Diptera) and by Mahloul et al (2016) on Caelifera (Orthoptera).…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal patterns of ground beetle diversity (Coleoptera: Carabidae) in a Ramsar wetland (Chott Tinsilt) of Algeria

Amri¹,

Neffar²,

Ouchtati³

et al. 2019

Turk J Zool

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The aim of this study was to investigate the biodiversity and structure of the Carabidae community living at the edges of a Ramsar wetland in northeastern Algeria, as well as to analyze the effect of environmental variables on this Carabidae community. The study site included two stations (T1, T2). From November 2016 to October 2017, the carabidofauna was sampled fortnightly using pitfall traps. The structure and diversity of the beetle community were measured using nonparametric indices, i.e. species richness, diversity indices, accumulation curves, and similarity analysis. Generalized linear mixed effects models (GLMM) were applied to examine the spatiotemporal variation of species abundance, and to test the effects of edaphic variables and vegetation cover on the variation of beetle abundance and species richness. In total, 1121 individuals were caught, belonging to 42 species, 24 genera, and 12 subfamilies; most of the species belonged to the subfamily Harpalinae (33.33%). GLMM demonstrated that abundance of Carabidae varied significantly between the stations and over the months studied. GLMM also showed significant effects of environmental factors on the abundance and species richness of the community. An important taxonomic diversity of the ground beetle community was found, which is highly related to environmental variables.

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Haloarcula

Ventosa¹,

Haba²,

Sánchez‐Porro³

2019

Bergey's Manual of Systematics of Archaea and Bacteria

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Ha.lo.ar'cu.la. Gr. n. hals, halos salt; L. fem. n. arcula small box; N.L. fem. n. Haloarcula salt (‐requiring) small box. Euryarchaeota / Halobacteria / Halobacteriales / Haloarculaceae / Haloarcula The genus Haloarcula is classified within the family Haloarculaceae and the order Halobacteriales , in the class Halobacteria . The cells are rods to pleomorphic (triangles, squares, and irregular disks) and stain Gram‐negative. Colonies are red to red‐orange or red‐pink pigmented. They are neutrophilic (optimal growth at pH 7.0–7.5) and extremely halophilic Archaea, growing in media containing 1.7–5.2 M NaCl. Polar lipids include phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP‐Me), phosphatidylglycerol sulfate (PGS), and characteristically the glycolipid glucosyl–mannosyl–glucosyl diether (TGD‐2). The DNA G + C range is 60.1–65 mol%. Currently, the genus includes nine species: Haloarcula vallismortis (type species of the genus), Haloarcula amylolytica , Haloarcula argentinensis , Haloarcula hispanica , Haloarcula japonica , Haloarcula marismortui , Haloarcula quadrata , Haloarcula salaria , and Haloarcula tradensis . The strains of these species were isolated from salt lakes, salterns, saline soils, and salt samples. DNA G + C content (mol % ) : 60.1–65 (the DNA may contain a major and a minor component). Type species : Haloarcula vallismortis (Gonzalez, Gutierrez and Ramirez 1978) Torreblanca, Rodriguez‐Valera, Juez, Ventosa, Kamekura and Kates 1986b, 573 VP (Effective publication: Torreblanca, Rodriguez‐Valera, Juez, Ventosa, Kamekura and Kates 1986a, 98; basonym: Halobacterium vallismortis Gonzalez, Gutierrez and Ramirez 1978, 710).

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Diversity and bioprospecting of extremely halophilic archaea isolated from Algerian arid and semi-arid wetland ecosystems for halophilic-active hydrolytic enzymes

Cited by 65 publications

References 50 publications

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Spatiotemporal patterns of ground beetle diversity (Coleoptera: Carabidae) in a Ramsar wetland (Chott Tinsilt) of Algeria

Haloarcula

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