Diversity and Characterization of Endophytic Bacteria Associated with Tidal Flat Plants and their Antagonistic Effects on Oomycetous Plant Pathogens

Biological Control of Oomycetous Plant Pathogens: A Review

“…also controlled oomycetes. Moreover, about 9% of the total number of isolated bacteria identified as Firmicutes, α-Proteobacteria, γ-Proteobacteria and Actinomycetes exhibited anti-oomycetic activity (Bibi et al 2012). Mechanisms of anti-oomycetic activity are mainly due to colonization, antibiotic production, hyphal lysis, sporangium abortion, oospore parasitism and siderophore production (Buysens et al 1996, Broadbent et al 1971, Drapeau et al 1973, Honor & Tsao 1973, Broadbent & Baker 1974, Wynn & Epton 1979.…”

Endophytic Microbiota Associated with the Root Tips and Leaves of Baccharis dracunculifolia

“…The most abundant phyla were Proteobacteria (galled plant roots= 53.8%, galled leaves= 54.2% and healthy leaves= 63.5%), Actinobacteria (galled plant roots= 28.1%, galled leaves= 17% and healthy leaves= 6.1%), Firmicutes (galled plant roots= 4.5%, galled leaves= 11.2% and healthy leaves= 10.1%), Acidobacteria (5.3%, exclusively from galled plant roots), and Nitrospirae (galled leaves =10.8% and healthy leaves= 11.2%). The two phyla Proteobacteria and Actinobacteria are consistently found on different plant species[ [35][36][37], although their frequency and composition vary enormously among the studied species. Other phyla that were found in the studied tissues of B. dracunculifolia, but at lower proportions (with abundances ranging from 1.1 to 4.5%), were: Bacteroidetes, Chloroflexi, and Verrucomicrobia (Fig.…”

Diversity and antagonistic potential of bacteria isolated from marine grass Halodule uninervis

“…Roots and leaves were also used for isolation of bacteria by washing several times with sterile distilled water to remove any contamination with soil. For isolation of endophytic bacteria from roots and leaves, sterilization procedure was carried out as described before (Bibi et al 2012). After the sterilization of roots and leaves, about 1.0 g of each segments of roots and leaves were crushed using sterile mortar and pestle.…”

“…The 16S rRNA gene fragment was amplified using bacterial universal primers 27F (5′-AGA GTT TGA TCC TGG CTC AG-3′) and 1492R (5′-GGT TAC CTT GTT ACG ACT T-3′). Amplifications were performed as described previously (Bibi et al 2012). The PCR products were purified using PCR purification kit (Thermo Scientific, Waltham, USA) and were sequenced by Macrogen (Seoul, Korea).…”

“…In past, for the discovery of a new compound, different marine samples from marine environment have been searched. Many studies have reported the antagonistic bacterial communities associated with marine plants suggesting their beneficial role in plants (Shin et al 2007;Bibi et al 2012). In this mutualistic relationship, microbial communities in marine environment help plant in different processes such as growth promotion, nitrogen fixation, remediation of different contaminants, and defense against different pathogenic microbes and in turn plant provide shelter to microbes (Singh and Reddy 2014).…”