Diversity and distribution of antibiotic resistance genes associated with road sediments transported in urban stormwater runoff

Geographic patterns and determinants of antibiotic resistomes in coastal sediments across complex ecological gradients

“…On the other hand, natural factors may also contribute to the gradient of ARG abundance from the bays to offshore area. For example, we found strong positive correlations between the proportion of clay and slit (particle-size <20 μm) and the total abundance of ARGs, corresponding to a higher abundance of ARGs detected in sediment particles with size <75 μm than those with greater size ( Zuo et al, 2022 ). This is probably due to the enhanced adsorption of microorganisms and extracellular DNA (including ARGs) onto the particles with smaller size ( Jackson and Weeks, 2008 ; Czekalski et al, 2014 ).…”

Antibiotic resistance in urban stormwater: a review of the dissemination of resistance elements, their impact, and management opportunities

“…[48][49][50] Primarily, previous research has sought to describe the resistance elements in stormwater runoff by sampling at stormwater outfalls, where runoff from a larger urban area is piped and discharged into a water body (e.g., lake, river, reservoir) at one central point (Table 1). [48][49][50][51][52][53][54][55][56][57][58][59][60][61] The stormwater disseminated from outfalls is mostly runoff collected via overland flow, but stormwater can include sewage inputs as it travels through a storm sewer system. 56 The presence of sewage in stormwater can significantly alter the ARGs present and their concentration at an outfall.…”

“…When heterotrophic plate counting was utilized to investigate ARB in stormwater, the concentration of tetracycline, β-lactam, and sulfonamide resistant bacteria ranged between 8.92 × 10 3 -2.33 × 10 5 CFU L −1 . 49 Analyzing species beyond E. coli is especially beneficial in reporting how widespread resistance is in stormwater bacterium; carbapenem resistance, for example, was reported to be especially prevalent in Gram-negative bacteria, including opportunistic pathogens of humans Pseudomonas monteilii, P. fulva, Brevundimonas vesicularis, Enterococcus durans, Acinetobacter junii, A. johnsonii, and S. maltophilia. 54 2.1.3 The diversity of the urban stormwater runoff resistome.…”

“…As a result, only a few studies have sampled ARGs in stormwater as direct runoff from urban surfaces (Table 1). 48–50 Primarily, previous research has sought to describe the resistance elements in stormwater runoff by sampling at stormwater outfalls, where runoff from a larger urban area is piped and discharged into a water body ( e.g. , lake, river, reservoir) at one central point (Table 1).…”

Seasonal and spatial patterns differ between intracellular and extracellular antibiotic resistance genes in urban stormwater runoff

“…7,8 Such pollutants are known to select for or correlate with the occurrence of ARGs and ARB. [9][10][11] The incidence of environmental stressors with indigenous and exogenous bacteria, including human commensal bacteria and pathogens, presents the possibility of an increased prevalence of ARGs and ARB in stormwater runoff and receiving environments throughout an urban watershed.…”