Diversity and distribution of bacterioplankton in the coastal upwelling waters off Hainan Island, China

The Effects of Intraguild Predation on Phytoplankton Assemblage Composition and Diversity: A Mesocosm Experiment

“…The 18S rRNA gene of all samples was amplified by polymerase chain reaction (PCR) using the EK-NSF573 5′-CGCGGTAATTCCAGCTCCA-3′ and Ek-NSR951 5′-TTGGYRAATGCTTTCGC-3′ primers [ 32 ], with a fragment length of approximately 560 bp [ 33 ]. High-throughput sequencing was conducted using the Illumina Miseq platform [ 23 ]. The PCR amplification reaction system consisted of a total volume of 25 μL, which included the following: 0.1 μL (20 pmol L −1 ) of the forward primer and 0.1 μL (20 pmol L −1 ) of the reverse primer, 3~5 μL of DNA template, 12.5 μL of 10× AccuPrime TM PCR buffer II, 0.5 μL of AccuPrime TM Taq high-fidelity DNA polymerase (Invitrogen, Carlsbad, CA, USA), and sterile deionized water added to a final volume of 25 μL.…”

“…SPSS software version 20.0 was used to perform one-way ANOVA to analyze differences in environmental indicators, the biomass of various zooplankton groups, Chl- a , and the alpha diversity of phytoplankton among different treatment systems [ 23 ]. The glm function in the glmmTMB v1.0.2.1 package in R 4.1.2 was used to conduct generalized linear mixed model analysis in studying the effects of fish, shrimp, and their combined effects on trophic cascades [ 4 ].…”

“…It should be noted that while microscopy-based methods are valuable for assessing phytoplankton diversity, they may not be able to provide a comprehensive characterization, especially phylogenetic diversity, of the entire assemblages [ 22 ]. Currently, high-throughput sequencing of environmental DNA (eDNA) has matured as a technology and has been used for monitoring biodiversity in lake ecosystems [ 23 ]. Compared with traditional methods of microscopy-based species identification, eDNA high-throughput sequencing has the advantages of high sensitivity; standardized methods; and low human, material, and time costs, making it possible to discover the vast diversity of phytoplankton in lake ecosystems, including microorganisms, and rare and fragile species [ 21 , 24 ].…”

Variations in bacterial community at Chlorophyll Maximum (C-Max) depths along the west coast of India due to seasonal changes in the primary productivity

“…NGS technologies have significantly enhanced the understanding of the active members within bacterial communities, revealing the ecological significance of rare phylotypes such as SAR11, Alteromonadales and Vibrionales in their response to environmental changes (Gutiérrez-Barral et al, 2021; Mena et al, 2022). Additionally, the impact of upwelling on microbial community composition has also been observed at C-Max depths (Gong et al, 2022). Collectively, these studies underscore the variability and complexity of bacterial community structure and abundance within upwelling zones shaped by distinct environmental factors and seasons.…”

Microbial degradation mechanism of historical silk revealed by proteomics and metabolomics

“…Gong et al used polarized infrared spectroscopy and total reection infrared spectroscopy to analyze the orientation and crystallinity of articial alkaline hydrolyzed silk and ancient silk fabrics, and obtained the pattern of change in the aggregation state of the bers. 9 Liu et al suggested that when silk fabrics are exposed to UV light and water, the amino acids in the amorphous zone are destroyed and the crystalline components become loose due to the presence of water, which leads to a reduction in the fracture strength. 10 Unlike chemical degradation where we can accelerate rates by adjusting the conditions (e.g., temperature, UV irradiation), we cannot accelerate biodegradation.…”