Diversity and distribution of the bioactive actinobacterial genus Salinispora from sponges along the Great Barrier Reef

Vidgen, Miranda E.; Hooper, John N. A.; Fuerst, John A.

doi:10.1007/s10482-011-9676-9

Cited by 22 publications

(16 citation statements)

References 39 publications

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“…are most frequently reported from marine sediments, however this may represent sampling bias. They have also been reported from an ascidian 28 , seaweeds 13 , and marine sponges 27, 29 . To date, there is no evidence that plant or invertebrate-associated populations are ecologically or evolutionarily distinct from those that occur in sediments.…”

Section: Discovery Of the Genusmentioning

confidence: 77%

“…The three species share approximately 99% 16S rRNA gene sequence identity and are not well resolved using this conserved phylogenetic marker 25 . However, less conserved loci have been used to generate well-supported phylogenies that clearly delineate the three species and reveal the sister relationship between S. tropica and S. pacifica relative to the more ancestral S. arenicola lineage 24–27 .…”

Section: Discovery Of the Genusmentioning

confidence: 99%

“…using phylogenetic approaches, it is well known that even minor differences in this locus can correspond to major differences in genome content, and thus detailed analyses have been performed to document all Salinispora 16S rRNA sequence variants reported to date 27, 32 . These efforts have required a careful monitoring of the position of all variable nucleotides relative to the level of conservation for that region of the gene and have provided a method to distinguish among 16S rRNA sequence variants or “sequence types” based on single nucleotide polymorphisms, each of which has been assigned a letter.…”

Section: Salinispora Natural Productsmentioning

confidence: 99%

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The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery

2015

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This review covers the initial discovery of the marine actinomycete genus Salinispora through its development as a model for natural product research. A focus is placed on the novel chemical structures reported with reference to their biological activities and the synthetic and biosynthetic studies they have inspired. The time line of discoveries progresses from more traditional bioassay-guided approaches through the application of genome mining and genetic engineering techniques that target the products of specific biosynthetic gene clusters. This overview exemplifies the extraordinary biosynthetic diversity that can emanate from a narrowly defined genus and supports future efforts to explore marine taxa in the search for novel natural products.

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Section: Discovery Of the Genusmentioning

confidence: 77%

Section: Discovery Of the Genusmentioning

confidence: 99%

Section: Salinispora Natural Productsmentioning

confidence: 99%

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The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery

2015

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“…Salinispora isolates were collected at various locations along the Great Barrier Reef (GBR), Queensland, Australia, an area frequently studied for its tropical marine ecosystem (Figure 1). The isolation and taxonomic identification of Salinispora isolates has been previously reported [9], [33]. Strains used for rifamycin W identification were reported by Ng and co-workers [34].…”

Section: Methodsmentioning

confidence: 98%

Discovering the Recondite Secondary Metabolome Spectrum of Salinispora Species: A Study of Inter-Species Diversity

et al. 2014

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Patterns of inter-species secondary metabolite production by bacteria can provide valuable information relating to species ecology and evolution. The complex nature of this chemical diversity has previously been probed via directed analyses of a small number of compounds, identified through targeted assays rather than more comprehensive biochemical profiling approaches such as metabolomics. Insights into ecological and evolutionary relationships within bacterial genera can be derived through comparative analysis of broader secondary metabolite patterns, and this can also eventually assist biodiscovery search strategies for new natural products. Here, we investigated the species-level chemical diversity of the two marine actinobacterial species Salinispora arenicola and Salinispora pacifica, isolated from sponges distributed across the Great Barrier Reef (GBR), via their secondary metabolite profiles using LC-MS-based metabolomics. The chemical profiles of these two species were obtained by UHPLC-QToF-MS based metabolic profiling. The resultant data were interrogated using multivariate data analysis methods to compare their (bio)chemical profiles. We found a high level of inter-species diversity in strains from these two bacterial species. We also found rifamycins and saliniketals were produced exclusively by S. arenicola species, as the main secondary metabolites differentiating the two species. Furthermore, the discovery of 57 candidate compounds greatly increases the small number of secondary metabolites previously known to be produced by these species. In addition, we report the production of rifamycin O and W, a key group of ansamycin compounds, in S. arenicola for the first time. Species of the marine actinobacteria harbour a much wider spectrum of secondary metabolites than suspected, and this knowledge may prove a rich field for biodiscovery as well as a database for understanding relationships between speciation, evolution and chemical ecology.

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“…tropica , and S . pacifica ) within the family Micromonosporeaceae 21, 22 whose relationships could not be confidently resolved based on 16S rRNA gene phylogeny 23, 24 . The genus is a rich source of structurally diverse natural products 25 , and there is evidence that certain compounds 26 and their associated biosynthetic gene clusters (BGCs) 27 are fixed at the species level.…”

Section: Introductionmentioning

confidence: 99%

Phylogenomic Insight into Salinispora (Bacteria, Actinobacteria) Species Designations

Millán-Aguiñaga

Chavarría

Ugalde

et al. 2017

Sci Rep

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Bacteria represent the most genetically diverse kingdom of life. While great progress has been made in describing this diversity, it remains difficult to identify the phylogenetic and ecological characteristics that delineate groups of bacteria that possess species-like properties. One major challenge associated with species delineations is that not all shared genes have the same evolutionary history, and thus the choice of loci can have a major impact on phylogenetic reconstruction. Sequencing the genomes of large numbers of closely related strains provides new opportunities to distinguish ancestral from acquired alleles and assess the effects of recombination on phylogenetic inference. Here we analyzed the genomes of 119 strains of the marine actinomycete genus Salinispora, which is currently comprised of three named species that share 99% 16S rRNA gene sequence identity. While 63% of the core genome showed evidence of recombination, this had no effect on species-level phylogenomic resolution. Recombination did however blur intra-species relationships and biogeographic resolution. The genome-wide average nucleotide identity provided a new perspective on Salinispora diversity, revealing as many as seven new species. Patterns of orthologous group distributions reveal a genetic basis to delineation the candidate taxa and insight into the levels of genetic cohesion associated with bacterial species.

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Diversity and distribution of the bioactive actinobacterial genus Salinispora from sponges along the Great Barrier Reef

Cited by 22 publications

References 39 publications

The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery

The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery

Discovering the Recondite Secondary Metabolome Spectrum of Salinispora Species: A Study of Inter-Species Diversity

Phylogenomic Insight into Salinispora (Bacteria, Actinobacteria) Species Designations

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