Diversity and dynamics of plant genome size: An example of polysomaty from a cytogenetic study of Tahitian vanilla (Vanilla ×tahitensis, Orchidaceae)

Lepers-Andrzejewski, Sandra; Siljak‐Yakovlev, Sonja; Brown, Spencer C.; Wong, Maurice; Dron, Michel

doi:10.3732/ajb.1000415

Cited by 46 publications

(30 citation statements)

References 36 publications

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“…The number of linkage groups was higher than expected according to the haploid number of chromosomes found in diploid tahitian vanilla ( n = 15; Lepers‐Andrzejewski et al, 2011). Furthermore two linkage groups were of less than 20 cM, indicating a lack of mapping markers and marker sparse regions.…”

Section: Discussioncontrasting

confidence: 62%

“…Three ploidy levels, ranging from diploid to tetraploid, have been observed among accessions of tahitian vanilla (Lepers‐Andrzejewski et al, 2011). Tahiti, which is diploid, is the morphotype most common in French Polynesia.…”

Section: Discussionmentioning

confidence: 99%

“…Polyploidy was also reported among these V. × tahitensis accessions (Duval et al, 2006). Recently we revealed three levels of ploidy among tahitian vanilla: diploid, triploid, and tetraploid with 2C DNA content averaging respectively 5.23, 7.82, and 10.27 pg, corresponding to a monoploid C‐value of 2557 Mb (Lepers‐Andrzejewski et al, 2011).…”

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confidence: 99%

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Genetic Linkage Map and Diversity Analysis of Tahitian Vanilla (Vanilla ×tahitensis, Orchidaceae)

et al. 2012

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The first vanilla genetic linkage map was constructed by analyzing the progeny of an interspecific cross between Vanilla xtahitensis J. W. Moore (pro sp.) and Vanilla pompona Schiede. The two-way pseudotestcross mapping strategy was used and a total of 225 amplified fragment length polymorphism (AFLP) markers were mapped on 18 linkage groups covering 1035.85 cM. Thirteen tahitian vanilla morphotypes, one Vanilla planifolia Andrews and recent hybrids were compared by measuring seven morphological traits and by genotyping, with 539 AFLP fragments. The morphotypes studied showed relatively homogenous stem traits but differed in their bean and leaf traits as in their AFLP pattern. Genetic variability and AFLP genotyping comparison suggest a diversification in French Polynesia based on sexual reproduction and are consistent with the hypothesis of a single introduction event. These results are the first step for vanilla breeding program and will be also useful for tahitian vanilla resource preservation.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

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Genetic Linkage Map and Diversity Analysis of Tahitian Vanilla (Vanilla ×tahitensis, Orchidaceae)

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“…1; supplementary material Figs S1, S2) ). This appears to be a general rule for higher plant endoreduplication, with a notable exception being the developmentally regulated partial endoreduplication observed in orchids (Lepers-Andrzejewski et al, 2011).…”

Section: Research Articlementioning

confidence: 99%

Evidence for karyoplasmic homeostasis during endoreduplication and a ploidy-dependent increase in gene transcription during tomato fruit growth

et al. 2012

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SUMMARYEndopolyploidy is a widespread process that corresponds to the amplification of the genome in the absence of mitosis. In tomato, very high ploidy levels (up to 256C) are reached during fruit development, concomitant with very large cell sizes. Using cellular approaches (fluorescence and electron microscopy) we provide a structural analysis of endoreduplicated nuclei at the level of chromatin and nucleolar organisation, nuclear shape and relationship with other cellular organelles such as mitochondria. We demonstrate that endopolyploidy in pericarp leads to the formation of polytene chromosomes and markedly affects nuclear structure. Nuclei manifest a complex shape, with numerous deep grooves that are filled with mitochondria, affording a fairly constant ratio between nuclear surface and nuclear volume. We provide the first direct evidence that endopolyploidy plays a role in increased transcription of rRNA and mRNA on a per-nucleus basis. Overall, our results provide quantitative evidence in favour of the karyoplasmic theory and show that endoreduplication is associated with complex cellular organisation during tomato fruit development.

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“…[26] Solanum lycopersicum L. "Montfavet 63/5 (2C=1.99 pg) was used as internal standard. [27] In order to remove large tissue debris and fragments, the nuclei suspension was filtered through nylon mesh, with 50 μm size of pore and stained with 50 μg/mL propidium iodide (Sigma Chemical Co. St. Louis, USA). At least 5 individuals with 5000 to 10000 analyzed nuclei were measured to obtain the mean DNA content.…”

Section: Chromosome Number and Genome Size Evaluationmentioning

confidence: 99%

Centaurea rupestris L.: Cytogenetics, Essential Oil Chemistry and Biological Activity

Carev¹,

Maravić²,

Bektašević³

et al. 2018

Croat. Chem. Acta

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Centaurea species are used in eastern Mediterranean ethnopharmacology due to variety of bioactive compounds they comprise. Aim of this work was to characterize the Centaurea rupestris L. hydrodistilled essential oil chemical composition and test its biological activity: antimicrobial effect, antioxidant potential and inhibition of cholinesterases. Plant material authentication was by chromosome number counting and genome size assessment with the flow cytometry. Hydrodistilled essential oils were characterized using gas chromatography/mass spectrometry technique GC-MS and GC-FID. The antimicrobial effect was tested using disk diffusion and microdilution methods, antioxidant potential was tested with DPPH and FRAP methods and cholinesterases inhibition was tested with Ellman method. Genome size for C. rupestris species: sample A presented 2C=3.60 (0.10) pg and sample B 2C=3.62 (0.08) pg. The chromosome number was 2n=20 for both samples. The main essential oil constituents in isolated sample A oil, detected with GC-MS and GC-FID were: germacrene D (24.3 %), heptacosane (14.4 %), phytol (6.7 %), β-caryophyllene (5.0 %) and pentacosane (4.5 %). Sample B essential oil had the main constituents: hexadecanoic acid (18.7 %), heptacosane (13.8 %), α-linolenic acid (11.8 %), nonacosane (7.8 %) and germacrene D (5.4 %). Both samples of oil showed broad spectrum antimicrobial effect with good activity against emerging Gram-positive and Gram-negative opportunistic pathogens and pathogenic fungi which indicates the pharmaceutical potential of the C. rupestris essential oil.

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Diversity and dynamics of plant genome size: An example of polysomaty from a cytogenetic study of Tahitian vanilla (Vanilla ×tahitensis, Orchidaceae)

Cited by 46 publications

References 36 publications

Genetic Linkage Map and Diversity Analysis of Tahitian Vanilla (Vanilla ×tahitensis, Orchidaceae)

Genetic Linkage Map and Diversity Analysis of Tahitian Vanilla (Vanilla ×tahitensis, Orchidaceae)

Evidence for karyoplasmic homeostasis during endoreduplication and a ploidy-dependent increase in gene transcription during tomato fruit growth

Centaurea rupestris L.: Cytogenetics, Essential Oil Chemistry and Biological Activity

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